Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between Aspergillus fumigatus and Haemophilus influenza?
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Clinical Specimens
2.2. Methods
2.2.1. Direct and Histopathologic Examination and Microbiological Cultures
2.2.2. DNA Extraction Protocol for High Throughput Sequencing
2.2.3. Library Preparation and Sequencing
2.2.4. Taxonomic Assignment, Diversity
2.2.5. Statistical Analysis
3. Results
3.1. Patient Characteristics
3.2. 16S, ITS1 and ITS2 Targeted Amplicon Sequencing Results
3.3. Comparison of ITS1 and ITS2 Regions for Mycobiota Analysis
3.4. Analysis of Microbial Diversity in Fungal Ball (FB) and Non-Fungal Ball Chronic Rhinosinusitis (NFBRS)
4. Discussion
4.1. Targeting ITS1 or ITS2 to Study Fungal Diversity
4.2. Fungal Diversity in FBRS
4.3. Hypothesis to Explain the Poor Positive Culture Rate of FBRS Samples
4.4. Could FBRS Result from Aspergillus–Haemophilus Microbial Interaction?
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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FB | NFBRS | p | |
---|---|---|---|
Age (+/− SD) (years) | 60 (±13) | 55 (±14) | 0.45 |
Sex ratio H/F | 0.58 | 0.75 | 0.99 |
Typeofsinusitisn (%) | 0.18 | ||
Maxillary | 24 (63.2) | 3 (42.9) | |
Frontal | 1 (2.6) | 1 (14.3) | |
Ethmoidal | 1 (2.6) | 1 (14.3) | |
Sphenoidal | 7 (18.4) | 0 (0) | |
Pansinusitis | 5 (13.2) | 2 (28.6) | |
Immunocompromised status | 2 (5.1) | 1 (14.3) | 0.41 |
Mycology | |||
Positive direct examination | 37 (97.3) | 0 (0) | <0.001 |
Positive culture | 12 (31.6) | 0 (0) | 0.16 |
Bacteriology | |||
Culture performed | 14 (36.8) | 7 (100) | <0.001 |
Histology | |||
Performed | 26 (68.4) | 5 (71.4) | - |
Hyphae observed | 20 (52.6) | 0 (0) | - |
ITS1 | ITS2 | p | ||
---|---|---|---|---|
Before trimming | Minimum reads/sample | 47,418 | 26,384 | |
Maximum reads/sample | 76,196 | 67,095 | ||
Mean reads/sample | 129,894 | 131,564 | NS | |
After trimming | Minimum reads/sample | 14 | 2 | |
Maximum reads/sample | 35,815 | 60,335 | ||
Mean reads/sample | 5967 | 25,374 | <0.001 | |
>5000 reads/FB sample | 26.3% (10/38) | 97.4% (37/38) | ||
OTUs produced | 113 | 157 | NS | |
Mean OTUs/sample | 6 | 9 | ||
Taxa (genus level) | 48 | 57 | --- |
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Dellière, S.; Dannaoui, E.; Fieux, M.; Bonfils, P.; Gricourt, G.; Demontant, V.; Podglajen, I.; Woerther, P.-L.; Angebault, C.; Botterel, F. Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between Aspergillus fumigatus and Haemophilus influenza? J. Fungi 2021, 7, 550. https://doi.org/10.3390/jof7070550
Dellière S, Dannaoui E, Fieux M, Bonfils P, Gricourt G, Demontant V, Podglajen I, Woerther P-L, Angebault C, Botterel F. Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between Aspergillus fumigatus and Haemophilus influenza? Journal of Fungi. 2021; 7(7):550. https://doi.org/10.3390/jof7070550
Chicago/Turabian StyleDellière, Sarah, Eric Dannaoui, Maxime Fieux, Pierre Bonfils, Guillaume Gricourt, Vanessa Demontant, Isabelle Podglajen, Paul-Louis Woerther, Cécile Angebault, and Françoise Botterel. 2021. "Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between Aspergillus fumigatus and Haemophilus influenza?" Journal of Fungi 7, no. 7: 550. https://doi.org/10.3390/jof7070550