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Article

Genetic Relationships in the Toxin-Producing Fungal Endophyte, Alternaria oxytropis Using Polyketide Synthase and Non-Ribosomal Peptide Synthase Genes

1
Department of Entomology, Plant Pathology and Weed Science, New Mexico State University, Las Cruces, NM 88003, USA
2
Arts and Science Biology Department, University of Houston, Victoria, TX 77901, USA
3
Department of Plant Pathology, University of Kentucky, Lexington, KY 40508, USA
4
Poisonous Plant Laboratory, US Department of Agriculture, Logan, UT 84341, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Willem Melchers
J. Fungi 2021, 7(7), 538; https://doi.org/10.3390/jof7070538
Received: 7 June 2021 / Revised: 29 June 2021 / Accepted: 30 June 2021 / Published: 6 July 2021
(This article belongs to the Section Fungal Genomics, Genetics and Molecular Biology)
The legume Oxytropis sericea hosts a fungal endophyte, Alternaria oxytropis, which produces secondary metabolites (SM), including the toxin swainsonine. Polyketide synthase (PKS) and non-ribosomal peptide synthase (NRPS) enzymes are associated with biosynthesis of fungal SM. To better understand the origins of the SM, an unannotated genome of A. oxytropis was assessed for protein sequences similar to known PKS and NRPS enzymes of fungi. Contigs exhibiting identity with known genes were analyzed at nucleotide and protein levels using available databases. Software were used to identify PKS and NRPS domains and predict identity and function. Confirmation of sequence for selected gene sequences was accomplished using PCR. Thirteen PKS, 5 NRPS, and 4 PKS-NRPS hybrids were identified and characterized with functions including swainsonine and melanin biosynthesis. Phylogenetic relationships among closest amino acid matches with Alternaria spp. were identified for seven highly conserved PKS and NRPS, including melanin synthesis. Three PKS and NRPS were most closely related to other fungi within the Pleosporaceae family, while five PKS and PKS-NRPS were closely related to fungi in the Pleosporales order. However, seven PKS and PKS-NRPS showed no identity with fungi in the Pleosporales or the class Dothideomycetes, suggesting a different evolutionary origin for those genes. View Full-Text
Keywords: Alternaria sect. Undifilum; polyketide synthase; secondary metabolite; nonribosomal peptide synthase; swainsonine biosynthesis Alternaria sect. Undifilum; polyketide synthase; secondary metabolite; nonribosomal peptide synthase; swainsonine biosynthesis
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MDPI and ACS Style

Creamer, R.; Hille, D.B.; Neyaz, M.; Nusayr, T.; Schardl, C.L.; Cook, D. Genetic Relationships in the Toxin-Producing Fungal Endophyte, Alternaria oxytropis Using Polyketide Synthase and Non-Ribosomal Peptide Synthase Genes. J. Fungi 2021, 7, 538. https://doi.org/10.3390/jof7070538

AMA Style

Creamer R, Hille DB, Neyaz M, Nusayr T, Schardl CL, Cook D. Genetic Relationships in the Toxin-Producing Fungal Endophyte, Alternaria oxytropis Using Polyketide Synthase and Non-Ribosomal Peptide Synthase Genes. Journal of Fungi. 2021; 7(7):538. https://doi.org/10.3390/jof7070538

Chicago/Turabian Style

Creamer, Rebecca, Deana B. Hille, Marwa Neyaz, Tesneem Nusayr, Christopher L. Schardl, and Daniel Cook. 2021. "Genetic Relationships in the Toxin-Producing Fungal Endophyte, Alternaria oxytropis Using Polyketide Synthase and Non-Ribosomal Peptide Synthase Genes" Journal of Fungi 7, no. 7: 538. https://doi.org/10.3390/jof7070538

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