Oxidative Stress Causes Vacuolar Fragmentation in the Human Fungal Pathogen Cryptococcus neoformans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Media
2.2. Construction of Strains Expressing the Sod1-GFP or the Sod2-GFP Fusion Protein
2.3. Construction of the fab1 Knock-Out Mutant
2.4. Confocal Fluorescence Microscopy
2.5. Flow Cytometric Analysis
2.6. Western Blot Analysis
2.7. Superoxide Dismutase Activity Assay
3. Results
3.1. Localization of Sod2 Is Influenced by Iron and Copper, and Iron Levels Impact the Expression and Activity of Sod2
3.2. Deletion of SOD Influences Expression of Iron Regulators as Well as Vacuole Morphology
3.3. Oxidative Stress Induces Vacuole Fragmentation
3.4. Vacuole Fragmentation Is Also Influenced by Fab1 and TORC1
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, D.; Song, M.; Do, E.; Choi, Y.; Kronstad, J.W.; Jung, W.H. Oxidative Stress Causes Vacuolar Fragmentation in the Human Fungal Pathogen Cryptococcus neoformans. J. Fungi 2021, 7, 523. https://doi.org/10.3390/jof7070523
Kim D, Song M, Do E, Choi Y, Kronstad JW, Jung WH. Oxidative Stress Causes Vacuolar Fragmentation in the Human Fungal Pathogen Cryptococcus neoformans. Journal of Fungi. 2021; 7(7):523. https://doi.org/10.3390/jof7070523
Chicago/Turabian StyleKim, Donghyeun, Moonyong Song, Eunsoo Do, Yoojeong Choi, James W. Kronstad, and Won Hee Jung. 2021. "Oxidative Stress Causes Vacuolar Fragmentation in the Human Fungal Pathogen Cryptococcus neoformans" Journal of Fungi 7, no. 7: 523. https://doi.org/10.3390/jof7070523