Cultivable Yeast Microbiota from the Marine Fish Species Genypterus chilensis and Seriolella violacea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fish, Intestinal Collection and Ethics Statement
2.2. Isolation of Yeasts from Intestinal Samples by Culture Method
2.3. Yeast DNA Extraction and Identification
2.4. Bioinformatic Workflow
2.5. Enzymatic Characterization of Yeasts
3. Results
3.1. Identification and Phylogeny of Isolated Yeasts
3.2. Enzymatic Characterization of Yeasts
4. Discussions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fish Species | Fish Specimen | Isolate ID | Length of the Sequence (bp) | Closest Relative (NCBI Access Code of the Best Match) | Identity (%) | Query Cover (%) |
---|---|---|---|---|---|---|
S. violacea | 1 | 254 | 960 | Candida palmioleophila (KJ705005.1) a | 99.8 | 100 |
1 | 276 | 990 | Candida palmioleophila (KJ705005.1) a | 99.7 | 100 | |
1 | 273 | 972 | Candida palmioleophila (KJ705005.1) a | 99.5 | 100 | |
1 | 264 | 1029 | Candida palmioleophila (KJ705005.1) a | 99.8 | 100 | |
1 | 281 | 925 | Candida palmioleophila (KJ705005.1) a | 99.9 | 100 | |
1 | 267 | 1028 | Candida palmioleophila (KJ705005.1) a | 99.3 | 100 | |
1 | 259 | 1037 | Candida palmioleophila (KJ705005.1) a | 99.9 | 100 | |
S. violacea | 2 | 284 | 979 | Rhodotorula mucilaginosa (MN006818.1) b | 99.7 | 99 |
S. violacea | 3 | 300 | 976 | Rhodotorula dairenensis (AB026010.2) b | 99.6 | 100 |
S. violacea | 4 | 285 | 1011 | Cystobasidium slooffiae (AB025994.2) b | 99.8 | 99 |
S. violacea | 5 | 286 | 799 | Cystobasidium slooffiae (AB025994.2) b | 99.6 | 100 |
G. chilensis | 1 | 301 | 626 | Candida sp. (KP794187.1 and KY101952.1) a | 99.3 99.3 | 94 94 |
1 | 302 | 1065 | Filobasidium sp. (KX067801.1) b | 94.2 | 97 | |
G. chilensis | 2 | 304 | 841 | Diutina mesorugosa (KY464166.1) a | 99.9 | 100 |
G. chilensis | 3 | 305 | 1018 | Rhodotorula mucilaginosa (MN006818.1) b | 99.8 | 99 |
G. chilensis | 4 | 306 | 919 | Yamadazyma sp. (JQ247716.1) a | 94.3 | 100 |
G. chilensis | 5 | 307 | 821 | Candida pseudorugosa (KT336718.1) a Diutina pseudorugosa (MK394157.1) a | 97.7 97.7 | 99 99 |
5 | 308 | 828 | Candida pseudorugosa (KT336718.1) a Diutina pseudorugosa (MK394157.1) a | 98.6 98.6 | 96 96 | |
5 | 309 | 849 | Candida pseudorugosa (KT336718.1) a Diutina pseudorugosa (MK394157.1) a | 97.0 97.0 | 100 100 | |
5 | 310 | 803 | Candida pseudorugosa (KT336718.1) a Diutina pseudorugosa (MK394157.1) a | 98.1 98.1 | 100 100 |
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Valderrama, B.; Ruiz, J.J.; Gutiérrez, M.S.; Alveal, K.; Caruffo, M.; Oliva, M.; Flores, H.; Silva, A.; Toro, M.; Reyes-Jara, A.; et al. Cultivable Yeast Microbiota from the Marine Fish Species Genypterus chilensis and Seriolella violacea. J. Fungi 2021, 7, 515. https://doi.org/10.3390/jof7070515
Valderrama B, Ruiz JJ, Gutiérrez MS, Alveal K, Caruffo M, Oliva M, Flores H, Silva A, Toro M, Reyes-Jara A, et al. Cultivable Yeast Microbiota from the Marine Fish Species Genypterus chilensis and Seriolella violacea. Journal of Fungi. 2021; 7(7):515. https://doi.org/10.3390/jof7070515
Chicago/Turabian StyleValderrama, Benjamín, José J. Ruiz, María Soledad Gutiérrez, Katherine Alveal, Mario Caruffo, Marcia Oliva, Héctor Flores, Alfonso Silva, Magaly Toro, Angélica Reyes-Jara, and et al. 2021. "Cultivable Yeast Microbiota from the Marine Fish Species Genypterus chilensis and Seriolella violacea" Journal of Fungi 7, no. 7: 515. https://doi.org/10.3390/jof7070515