Next Article in Journal
The Glyoxylate Cycle Is Involved in White-Opaque Switching in Candida albicans
Next Article in Special Issue
Oral Candidosis: Pathophysiology and Best Practice for Diagnosis, Classification, and Successful Management
Previous Article in Journal
Nanocarriers of Miconazole or Fluconazole: Effects on Three-Species Candida Biofilms and Cytotoxic Effects In Vitro
Previous Article in Special Issue
Immune Cell Degranulation in Fungal Host Defence
Article

Mass Spectrometry-Based Proteomic and Immunoproteomic Analyses of the Candida albicans Hyphal Secretome Reveal Diagnostic Biomarker Candidates for Invasive Candidiasis

1
Department of Microbiology and Parasitology, Faculty of Pharmacy, Complutense University of Madrid and IRYCIS, 28040 Madrid, Spain
2
Institute for Medical Microbiology and Virology, University Medical Center Gottingen, Kreuzbergring 57, D-37075 Gottingen, Germany
3
Proteomics Facility, Faculty of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Joseph M. Bliss
J. Fungi 2021, 7(7), 501; https://doi.org/10.3390/jof7070501
Received: 27 May 2021 / Revised: 18 June 2021 / Accepted: 18 June 2021 / Published: 23 June 2021
(This article belongs to the Special Issue Host Defense against Fungi)
Invasive candidiasis (IC) is associated with high morbidity and mortality in hospitalized patients if not diagnosed early. Long-term use of central venous catheters is a predisposing factor for IC. Hyphal forms of Candida albicans (the major etiological agent of IC) are related to invasion of host tissues. The secreted proteins of hyphae are involved in virulence, host interaction, immune response, and immune evasion. To identify IC diagnostic biomarker candidates, we characterized the C. albicans hyphal secretome by gel-free proteomic analysis, and further assessed the antibody-reactivity patterns to this subproteome in serum pools from 12 patients with non-catheter-associated IC (ncIC), 11 patients with catheter-associated IC (cIC), and 11 non-IC patients. We identified 301 secreted hyphal proteins stratified to stem from the extracellular region, cell wall, cell surface, or intracellular compartments. ncIC and cIC patients had higher antibody levels to the hyphal secretome than non-IC patients. Seven secreted hyphal proteins were identified to be immunogenic (Bgl2, Eno1, Pgk1, Glx3, Sap5, Pra1 and Tdh3). Antibody-reactivity patterns to Bgl2, Eno1, Pgk1 and Glx3 discriminated IC patients from non-IC patients, while those to Sap5, Pra1 and Tdh3 differentiated between cIC and non-IC patients. These proteins may be useful for development of future IC diagnostic tests. View Full-Text
Keywords: Candida albicans; invasive candidiasis; secretome; secreted proteins; hypha; diagnosis; biomarkers; immunoproteomics; SERPA; serologic response Candida albicans; invasive candidiasis; secretome; secreted proteins; hypha; diagnosis; biomarkers; immunoproteomics; SERPA; serologic response
Show Figures

Figure 1

MDPI and ACS Style

Vaz, C.; Pitarch, A.; Gómez-Molero, E.; Amador-García, A.; Weig, M.; Bader, O.; Monteoliva, L.; Gil, C. Mass Spectrometry-Based Proteomic and Immunoproteomic Analyses of the Candida albicans Hyphal Secretome Reveal Diagnostic Biomarker Candidates for Invasive Candidiasis. J. Fungi 2021, 7, 501. https://doi.org/10.3390/jof7070501

AMA Style

Vaz C, Pitarch A, Gómez-Molero E, Amador-García A, Weig M, Bader O, Monteoliva L, Gil C. Mass Spectrometry-Based Proteomic and Immunoproteomic Analyses of the Candida albicans Hyphal Secretome Reveal Diagnostic Biomarker Candidates for Invasive Candidiasis. Journal of Fungi. 2021; 7(7):501. https://doi.org/10.3390/jof7070501

Chicago/Turabian Style

Vaz, Catarina, Aida Pitarch, Emilia Gómez-Molero, Ahinara Amador-García, Michael Weig, Oliver Bader, Lucía Monteoliva, and Concha Gil. 2021. "Mass Spectrometry-Based Proteomic and Immunoproteomic Analyses of the Candida albicans Hyphal Secretome Reveal Diagnostic Biomarker Candidates for Invasive Candidiasis" Journal of Fungi 7, no. 7: 501. https://doi.org/10.3390/jof7070501

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop