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Article

Influence of Galectin-3 on the Innate Immune Response during Experimental Cryptococcosis

1
Department of Biochemistry and Immunology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto 14049-900, SP, Brazil
2
Department of Cellular and Molecular Biology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto 14049-900, SP, Brazil
3
Department of Pathology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto 14049-900, SP, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Brian L. Wickes
J. Fungi 2021, 7(6), 492; https://doi.org/10.3390/jof7060492
Received: 11 May 2021 / Revised: 11 June 2021 / Accepted: 16 June 2021 / Published: 20 June 2021
(This article belongs to the Special Issue Molecular and Genetic Tools for Studying Cryptococcus spp.)
Cryptococcus neoformans, the causative agent of cryptococcosis, is the primary fungal pathogen that affects the immunocompromised individuals. Galectin-3 (Gal-3) is an animal lectin involved in both innate and adaptive immune responses. The present study aimed to evaluate the influence of Gal-3 on the C. neoformans infection. We performed histopathological and gene profile analysis of the innate antifungal immunity markers in the lungs, spleen, and brain of the wild-type (WT) and Gal-3 knockout (KO) mice during cryptococcosis. These findings suggest that Gal-3 absence does not cause significant histopathological alterations in the analyzed tissues. The expression profile of the genes related to innate antifungal immunity showed that the presence of cryptococcosis in the WT and Gal-3 KO animals, compared to their respective controls, promoted the upregulation of the pattern recognition receptor (PRR) responsive to mannose/chitin (mrc1) and a gene involved in inflammation (ccr5), as well as the downregulation of the genes related to signal transduction (card9, fos, ikbkb, jun) and PRRs (cd209a, colec12, nptx1). The absence of Gal-3, in fungal infection, a positively modulated gene involved in phagocytosis (sftpd) and negatively genes involved in signal transduction (syk and myd88), proinflammatory cytokines il-1β and il-12b and cd209a receptor. Therefore, our results suggest that Gal-3 may play an essential role in the development of antifungal immune responses against cryptococcosis. View Full-Text
Keywords: Cryptococcus neoformans; galectin-3 (Gal-3), innate antifungal immunity Cryptococcus neoformans; galectin-3 (Gal-3), innate antifungal immunity
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MDPI and ACS Style

Rezende, C.P.; Brito, P.K.M.O.; Da Silva, T.A.; Pessoni, A.M.; Ramalho, L.N.Z.; Almeida, F. Influence of Galectin-3 on the Innate Immune Response during Experimental Cryptococcosis. J. Fungi 2021, 7, 492. https://doi.org/10.3390/jof7060492

AMA Style

Rezende CP, Brito PKMO, Da Silva TA, Pessoni AM, Ramalho LNZ, Almeida F. Influence of Galectin-3 on the Innate Immune Response during Experimental Cryptococcosis. Journal of Fungi. 2021; 7(6):492. https://doi.org/10.3390/jof7060492

Chicago/Turabian Style

Rezende, Caroline P., Patricia K.M.O. Brito, Thiago A. Da Silva, Andre M. Pessoni, Leandra N.Z. Ramalho, and Fausto Almeida. 2021. "Influence of Galectin-3 on the Innate Immune Response during Experimental Cryptococcosis" Journal of Fungi 7, no. 6: 492. https://doi.org/10.3390/jof7060492

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