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Article

Comparison of Two Commercially Available qPCR Kits for the Detection of Candida auris

1
Institute for Medical Microbiology, Immunology and Hygiene, University Hospital of Cologne, University of Cologne, 50935 Cologne, Germany
2
German Centre for Infection Research, Partner Site Bonn-Cologne, 50937 Cologne, Germany
3
Institute for Medical Microbiology and Virology, Carl von Ossietzky University Oldenburg, 26129 Oldenburg, Germany
4
Institute for Hygiene and Microbiology, University of Würzburg, 97080 Würzburg, Germany
5
German National Reference Centre for Invasive Fungal Infections, Leibniz Institute for Natural Product Research and Infection Biology—Hans Knöll Institute, 07745 Jena, Germany
6
Center of Expertise in Mycology Radboud University Medical Center/Canisius Wilhelmina Hospital, 6532 SZ Nijmegen, The Netherlands
7
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, 6532 SZ Nijmegen, The Netherlands
*
Author to whom correspondence should be addressed.
These authors contribute equally to this work.
Academic Editor: Eleftherios Mylonakis
J. Fungi 2021, 7(2), 154; https://doi.org/10.3390/jof7020154
Received: 6 December 2020 / Revised: 7 February 2021 / Accepted: 18 February 2021 / Published: 22 February 2021
(This article belongs to the Special Issue Candida auris 2.0)
Candida auris is an emerging pathogen with resistance to many commonly used antifungal agents. Infections with C. auris require rapid and reliable detection methods to initiate successful medical treatment and contain hospital outbreaks. Conventional identification methods are prone to errors and can lead to misidentifications. PCR-based assays, in turn, can provide reliable results with low turnaround times. However, only limited data are available on the performance of commercially available assays for C. auris detection. In the present study, the two commercially available PCR assays AurisID (OLM, Newcastle Upon Tyne, UK) and Fungiplex Candida Auris RUO Real-Time PCR (Bruker, Bremen, Germany) were challenged with 29 C. auris isolates from all five clades and eight other Candida species as controls. AurisID reliably detected C. auris with a limit of detection (LoD) of 1 genome copies/reaction. However, false positive results were obtained with high DNA amounts of the closely related species C. haemulonii, C. duobushaemulonii and C. pseudohaemulonii. The Fungiplex Candida Auris RUO Real-Time PCR kit detected C. auris with an LoD of 9 copies/reaction. No false positive results were obtained with this assay. In addition, C. auris could also be detected in human blood samples spiked with pure fungal cultures by both kits. In summary, both kits could detect C. auris-DNA at low DNA concentrations but differed slightly in their limits of detection and specificity. View Full-Text
Keywords: qPCR; detection limits; sensitivity; strain specificity; commercial kits; Candida auris; Fungiplex Candida Auris; AurisID qPCR; detection limits; sensitivity; strain specificity; commercial kits; Candida auris; Fungiplex Candida Auris; AurisID
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MDPI and ACS Style

Sattler, J.; Noster, J.; Brunke, A.; Plum, G.; Wiegel, P.; Kurzai, O.; Meis, J.F.; Hamprecht, A. Comparison of Two Commercially Available qPCR Kits for the Detection of Candida auris. J. Fungi 2021, 7, 154. https://doi.org/10.3390/jof7020154

AMA Style

Sattler J, Noster J, Brunke A, Plum G, Wiegel P, Kurzai O, Meis JF, Hamprecht A. Comparison of Two Commercially Available qPCR Kits for the Detection of Candida auris. Journal of Fungi. 2021; 7(2):154. https://doi.org/10.3390/jof7020154

Chicago/Turabian Style

Sattler, Janko, Janina Noster, Anne Brunke, Georg Plum, Pia Wiegel, Oliver Kurzai, Jacques F. Meis, and Axel Hamprecht. 2021. "Comparison of Two Commercially Available qPCR Kits for the Detection of Candida auris" Journal of Fungi 7, no. 2: 154. https://doi.org/10.3390/jof7020154

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