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Article

The Heat Shock Protein 60 and Pap1 Participate in the Sporothrix schenckii-Host Interaction

by
Laura C. García-Carnero
1,
Roberta Salinas-Marín
2,
Nancy E. Lozoya-Pérez
1,
Katarzyna Wrobel
3,
Kazimierz Wrobel
3,
Iván Martínez-Duncker
2,
Gustavo A. Niño-Vega
1 and
Héctor M. Mora-Montes
1,*
1
Departamento de Biología, División de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s/n, col. Noria Alta, C.P., Guanajuato 36050, Gto., Mexico
2
Laboratorio de Glicobiología Humana y Diagnóstico Molecular, Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Mor., Mexico
3
Departamento de Química, División de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s/n, col. Noria Alta, C.P., Guanajuato 36050, Gto., Mexico
*
Author to whom correspondence should be addressed.
J. Fungi 2021, 7(11), 960; https://doi.org/10.3390/jof7110960
Submission received: 20 October 2021 / Revised: 9 November 2021 / Accepted: 10 November 2021 / Published: 12 November 2021
(This article belongs to the Special Issue Sporothrix and Sporotrichosis 2.0)
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Abstract

Sporothrixschenckii is one of the etiological agents of sporotrichosis, a worldwide-distributed subcutaneous mycosis. Its cell wall contains a glycoconjugate composed of rhamnose, mannose, glucuronic acid, and proteins, named peptidorhamnomannan, which harbors important Sporothrix-specific immunogenic epitopes. Although the peptidorhamnomannan carbohydrate moiety has been extensively studied, thus far, little is known about the protein core. Here, using LC-MS/MS, we analyzed the S.schenckii peptidorhamnomannan peptide fraction and generated mass signals of 325 proteins, most of them likely to be moonlighting proteins. Among the identified proteins, chaperonin GroEL/Hsp60 and the uncharacterized protein Pap1 were selected for further analysis. Both proteins were heterologously expressed in bacteria, and they showed adhesive properties to the extracellular matrix proteins laminin, elastin, fibrinogen, and fibronectin, although Pap1 also was bound to type-I and type-II collagen. The inoculation of concentrations higher than 40 μg of these proteins, separately, increased immune effectors in the hemolymph of Galleriamellonella larvae and protected animals from an S.schenckii lethal challenge. These observations were confirmed when yeast-like cells, pre-incubated with anti-rHsp60 or anti-rPap1 antibodies were used to inoculate larvae. The animals inoculated with pretreated cells showed increased survival rates when compared to the control groups. In conclusion, we report that Hsp60 and Pap1 are part of the cell wall peptidorhamnomannan, can bind extracellular matrix components, and contribute to the S.schenckii virulence. To our knowledge, this is the first report about moonlighting protein in the S.schenckii cell wall with an important role during the pathogen–host interaction.
Keywords: sporotrichosis; cell wall; adhesin; glycoprotein; recombinant protein; virulence; moonlighting protein sporotrichosis; cell wall; adhesin; glycoprotein; recombinant protein; virulence; moonlighting protein

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MDPI and ACS Style

García-Carnero, L.C.; Salinas-Marín, R.; Lozoya-Pérez, N.E.; Wrobel, K.; Wrobel, K.; Martínez-Duncker, I.; Niño-Vega, G.A.; Mora-Montes, H.M. The Heat Shock Protein 60 and Pap1 Participate in the Sporothrix schenckii-Host Interaction. J. Fungi 2021, 7, 960. https://doi.org/10.3390/jof7110960

AMA Style

García-Carnero LC, Salinas-Marín R, Lozoya-Pérez NE, Wrobel K, Wrobel K, Martínez-Duncker I, Niño-Vega GA, Mora-Montes HM. The Heat Shock Protein 60 and Pap1 Participate in the Sporothrix schenckii-Host Interaction. Journal of Fungi. 2021; 7(11):960. https://doi.org/10.3390/jof7110960

Chicago/Turabian Style

García-Carnero, Laura C., Roberta Salinas-Marín, Nancy E. Lozoya-Pérez, Katarzyna Wrobel, Kazimierz Wrobel, Iván Martínez-Duncker, Gustavo A. Niño-Vega, and Héctor M. Mora-Montes. 2021. "The Heat Shock Protein 60 and Pap1 Participate in the Sporothrix schenckii-Host Interaction" Journal of Fungi 7, no. 11: 960. https://doi.org/10.3390/jof7110960

APA Style

García-Carnero, L. C., Salinas-Marín, R., Lozoya-Pérez, N. E., Wrobel, K., Wrobel, K., Martínez-Duncker, I., Niño-Vega, G. A., & Mora-Montes, H. M. (2021). The Heat Shock Protein 60 and Pap1 Participate in the Sporothrix schenckii-Host Interaction. Journal of Fungi, 7(11), 960. https://doi.org/10.3390/jof7110960

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