Four New Species of Hemileccinum (Xerocomoideae, Boletaceae) from Southwestern China
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection and Morphological Study
2.2. Molecular Procedures and Phylogenetic Analyses
2.3. Phylogenetic Analyses
Species | Voucher | Locality | GenBank Accession Number | References | ||||
---|---|---|---|---|---|---|---|---|
ITS | nrLSU | rpb2 | rpb1 | tef1-α | ||||
Hemileccinum rugosum | KUN-HKAS84355 | China | - | KT990578 | KT990413 | KT990931 | KT990774 | [8] |
Hemileccinum rugosum | KUN-HKAS84970 | China | - | KT990577 | KT990412 | - | KT990773 | [8] |
Hemileccinum rugosum | KUN-HKAS50284 | China | - | KT990576 | KT990411 | - | KT990772 | [8] |
Hemileccinum subglabripes | MICH:KUO-07230802 | USA | - | MK601738 | MK766300 | - | MK721092 | [10] |
Hemileccinum subglabripes | MICH:KUO-07070702 | USA | - | MK601737 | MK766299 | - | MK721091 | [10] |
Hemileccinum subglabripes | MICH:KUO-08301402 | USA | - | MK601739 | MK766301 | - | MK721093 | [10] |
Hemileccinum subglabripes | 72206 | USA | - | KF030303 | - | KF030374 | KF030404 | [36] |
Hemileccinum subglabripes | 294169 | USA | MN128237 | - | - | - | - | from GenBank |
Hemileccinum subglabripes | 3660 | - | KM248936 | - | - | - | - | from GenBank |
Hemileccinum depilatum | 2137333 | USA | AY127032 | - | - | - | - | from GenBank |
Hemileccinum depilatum | AF2845 | Belgium | - | - | MG212633 | - | MG212591 | [37] |
Hemileccinum depilatum | Bd1 | - | - | AF139712 | - | - | - | [5] |
Hemileccinum impolitum | Bim 1 | Germany | - | AF139715 | - | KF030375 | JQ327034 | [36] |
Hemileccinum impolitum | 47698 | Portugal | AJ419187 | - | - | - | - | [38] |
Hemileccinum impolitum | BI57407 | Thailand | KM235997 | - | - | - | - | from GenBank |
Hemileccinum impolitum | BI57408 | Thailand | KM235998 | - | - | - | - | from GenBank |
Hemileccinum impolitum | 17173 | USA | JF907783 | - | - | - | - | [39] |
Hemileccinum impolitum | KUN-HKAS84869 | Germany | - | KT990575 | KT990410 | KT990930 | KT990771 | [8] |
Hemileccinum indecorum | KUN-HKAS63126 | China | - | KF112440 | - | - | - | [7] |
Hemileccinum indecorum | OR0863 | Thailand | - | - | MH614772 | - | MH614726 | [16] |
Hemileccinum rubropunctum | JLF56666 | USA | MH190826 | - | - | - | - | from GenBank |
Hemileccinum rubropunctum | MES256 | USA | FJ480428 | - | - | - | - | [40] |
Hemileccinum rubropunctum | NY-792788REH-8501 | USA | - | MK601768 | MK766327 | - | MK721122 | [10] |
Hemileccinum rubropunctum | NY-01193924REH-9597 | USA | - | MK601769 | MK766328 | - | MK721123 | [10] |
Hemileccinum sp. | KUN-HKAS53421 | China | - | KF112432 | KF112751 | KF112565 | KF112235 | [7] |
Hemileccinum hortonii | MICH KUO-07050706 | USA | - | MK601821 | MK766377 | - | MK721175 | [10] |
Hemileccinum albidum | KUN-HKAS87225 | China | MZ923777 | MZ923774 | MZ936317 | MZ936334 | MZ936351 | This study |
Hemileccinum albidum | KUN-HKAS83355 | China | MZ923778 | MZ923775 | MZ936321 | MZ936340 | MZ936357 | This study |
Hemileccinum albidum (T) | KUN-HKAS81120 | China | MZ923782 | MZ923766 | MZ936320 | MZ936339 | MZ936352 | This study |
Hemileccinum albidum | KUN-HKAS50503 | China | MZ923781 | MZ923767 | MZ936319 | MZ936335 | MZ936355 | This study |
Hemileccinum albidum | KUN-HKAS50350 | China | MZ923779 | MZ923768 | MZ936323 | MZ936342 | MZ936359 | This study |
Hemileccinum albidum | KUN-HKAS84554 | China | MZ923780 | - | MZ936318 | MZ936336 | MZ936358 | This study |
Hemileccinum albidum | KUN-HKAS85753 | China | MZ923786 | - | MZ936325 | MZ936337 | MZ936353 | This study |
Hemileccinum albidum | KUN-HKAS87105 | China | - | MZ923769 | MZ936327 | MZ936338 | MZ936356 | This study |
Hemileccinum albidum | KUN-HKAS83333 | China | MZ923784 | - | MZ936326 | MZ936344 | MZ936361 | This study |
Hemileccinum albidum | KUN-HKAS83400 | China | MZ923783 | MZ923770 | MZ936324 | MZ936341 | MZ936354 | This study |
Hemileccinum albidum | KUN-HKAS115749 | China | MZ923785 | - | MZ936322 | MZ936343 | MZ936360 | This study |
Hemileccinum brevisporum (T) | KUN-HKAS89150 | China | MZ923788 | MZ923764 | MZ936328 | MZ936345 | MZ936362 | This study |
Hemileccinum brevisporum | KUN-HKAS59445 | China | - | KT990579 | KT990414 | KT990932 | KT990775 | [8] |
Hemileccinum brevisporum | KUN-HKAS67896 | China | MZ923787 | MZ923765 | MZ936329 | MZ936346 | MZ936363 | This study |
Hemileccinum ferrugineipes (T) | KUN-HKAS115554 | China | MZ923792 | MZ923773 | MZ936330 | MZ936350 | MZ973011 | This study |
Hemileccinum ferrugineipes | KUN-HKAS75054 | China | - | KF112377 | KF112749 | KF112563 | KF112234 | [7] |
Hemileccinum ferrugineipes | KUN-HKAS93310 | China | MZ923791 | - | MZ936331 | MZ936347 | MZ973012 | This study |
Hemileccinum parvum | KUN-HKAS99764 | China | MZ923789 | MZ923771 | MZ936332 | MZ936349 | MZ973009 | This study |
Hemileccinum parvum (T) | KUN-HKAS115553 | China | MZ923790 | MZ923772 | MZ936333 | MZ936348 | MZ973010 | This study |
Heimioporus sp. | KUN-HKAS53451 | China | - | KF112345 | KF112805 | KF112616 | KF112226 | [7] |
Heimioporus aff. japonicus | KUN-HKAS52236 | China | - | KF112346 | KF112807 | KF112617 | KF112227 | [7] |
Heimioporus japonicas | KUN-HKAS52237 | China | - | KF112347 | KF112806 | KF112618 | KF112228 | [7] |
Aureoboletus tenuis | KUN-HKAS75104 | China | - | KT990518 | KT990359 | KT990897 | KT990722 | [8] |
Aureoboletus thibetanus | KUN-HKAS76655 | China | - | KF112420 | KF112752 | KF112626 | KF112236 | [7] |
Pulchroboletus roseoalbidus | AMB 12757 | Italy | - | NG_060126 | - | - | KJ729512 | [27] |
Alessioporus ichnusanus | AMB 12756 | Italy | - | NG_057044 | - | - | KJ729513 | [27] |
Phylloporus rubrosquamosus | KUN-HKAS52552 | China | - | KF112391 | KF112780 | - | KF112289 | [7] |
Phylloporus rubrosquamosus | KUN-HKAS54559 | China | NR120124 | NG_042668 | - | - | JQ967175 | [24,25] |
Phylloporus rubeolus | KUN-HKAS52573 | China | JQ967259 | JQ967216 | - | - | JQ967172 | [24,25] |
Xerocomus fraternus | KUN-HKAS55328 | China | - | KT990681 | KT990497 | - | KT990869 | [8] |
Xerocomus velutinus | KUN-HKAS68135 | China | - | KT990673 | - | KT991011 | KT990861 | [8] |
Hourangia cheoi | Yang 5153 | China | KP136997 | KP136947 | KP136975 | KP136966 | KP136924 | [26] |
Hourangia pumila | REH8063 | Indonesia | JQ003626 | NG_060636 | - | - | - | [28] |
Boletellus indistinctus | KUN-HKAS77623 | China | - | KT990531 | KT990371 | - | KT990733 | [8] |
Boletellus indistinctus | KUN-HKAS80681 | China | - | KT990532 | KT990368 | KT990903 | KT990734 | [8] |
Leccinum variicolor | KUN-HKAS57758 | China | - | KF112445 | KF112725 | KF112591 | KF112251 | [7] |
Leccinum aff. scabrum | KUN-HKAS57266 | China | - | KF112442 | KF112722 | KF112590 | KF112248 | [7] |
Leccinum monticola | KUN-HKAS76669 | China | - | KF112443 | KF112723 | KF112592 | KF112249 | [7] |
Leccinellum cremeum | KUN-HKAS90639 | China | - | - | KT990420 | KT990936 | KT990781 | [8] |
Leccinellum sp. | KUN-HKAS53410 | China | - | KT990585 | KT990421 | KT990937 | - | [8] |
3. Results
3.1. Molecular Phylogenetic Analysis
3.2. Taxonomy
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Key to known species of Hemileccinum in the world | |
1. Pileus surface pale brown, brown to reddish brown, more or less even | 2 |
1′. Pileus surface orange to reddish orange, distinctively wrinkled | 9 |
2. Pileus surface slightly subtomentose, without small conical to subconical to irregularly shaped squamules, known in subtropical and temperate regions in the Northern Hemisphere… | 3 |
2′. Pileus surface with whitish to dirty white, small conical to subconical to irregularly shaped squamules, known from tropical southeast Asia… | H. indecorum |
3. Basidioma varied in size, yellowish context without color change when bruised… | 4 |
3′. Basidioma usually small in size (≤ 4 cm in daim), the pale yellow context staining pale blue very slowly when bruised, known from subtropical areas… | H. parvum |
4. Stipe surface covered with obvious ornaments… | 5 |
4′. Stipe surface covered with small scales, not obvious | 6 |
5. Stipe surface ornamented with distinctly reddish brown longitudinal streaks, known from East Asia | H. ferrugineipes |
5′. Stipe surface often covered with red dense fine-grained scales, known from North America… | H. rubropunctum |
6. Basidiospores longer in length (> 11 µm), and in other parts of the world… | 7 |
6′. Basidiospores shorter in length (≤ 11 µm), and distributed in subtropical forests in southwestern China.… | H. brevisporum |
7. Stipe stout (> 2.5 cm in diam.); pileipellis an trichoderm, collapsed when mature, restricted to Europe | H. impolitum |
7′. Stipe slender (≤ 2.5 cm in diam.); pileipellis an hyphoepithelium | 8 |
8. Stipe surface whitish, covered with small, granular scales only at the base, distributed in subtropical China | H. albidum |
8′. Stipe surface yellowish, nearly smooth, covered with indistinctive tiny scales, known from eastern North America | H. subglabripes |
9. Basidiospores smaller (10–12 × 4–5 µm), distributed in subtropical and tropical China | H. rugosum |
9′. Basidiospores larger (≥ 12 µm in length), distributed in temperate regions… | 10 |
10. Basidiospores narrower (12.0–15.0 × 3.5–4.5 µm), known from North America… | H. hortonii |
10′. Basidiospores wider (12.0–15.0 × 5–6 µm), known from Europe… | H. depilatum |
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Li, M.-X.; Wu, G.; Yang, Z.L. Four New Species of Hemileccinum (Xerocomoideae, Boletaceae) from Southwestern China. J. Fungi 2021, 7, 823. https://doi.org/10.3390/jof7100823
Li M-X, Wu G, Yang ZL. Four New Species of Hemileccinum (Xerocomoideae, Boletaceae) from Southwestern China. Journal of Fungi. 2021; 7(10):823. https://doi.org/10.3390/jof7100823
Chicago/Turabian StyleLi, Mei-Xiang, Gang Wu, and Zhu L. Yang. 2021. "Four New Species of Hemileccinum (Xerocomoideae, Boletaceae) from Southwestern China" Journal of Fungi 7, no. 10: 823. https://doi.org/10.3390/jof7100823
APA StyleLi, M.-X., Wu, G., & Yang, Z. L. (2021). Four New Species of Hemileccinum (Xerocomoideae, Boletaceae) from Southwestern China. Journal of Fungi, 7(10), 823. https://doi.org/10.3390/jof7100823