Next Article in Journal
Of Mice and Fungi: Coccidioides spp. Distribution Models
Next Article in Special Issue
Risk Factors for Mortality among HIV-Infected Patients with Disseminated Histoplasmosis
Previous Article in Journal
Chronic Pulmonary Aspergillosis in Post Tuberculosis Patients in Indonesia and the Role of LDBio Aspergillus ICT as Part of the Diagnosis Scheme
Previous Article in Special Issue
Coronavirus Disease 2019 (COVID-19) in a Patient with Disseminated Histoplasmosis and HIV—A Case Report from Argentina and Literature Review
Open AccessArticle

Detection of Histoplasma DNA from Tissue Blocks by a Specific and a Broad-Range Real-Time PCR: Tools to Elucidate the Epidemiology of Histoplasmosis

1
Reference Laboratory for Cryptococcosis and Uncommon Invasive Fungal Infections, Division for Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute, 13353 Berlin, Germany
2
Vet Med Labor GmbH, Division of IDEXX Laboratories, 71636 Ludwigsburg, Germany
3
Department of Medicine A, Hematology, Oncology and Pulmonary Medicine, University Hospital Muenster, 48149 Muenster, Germany
4
Institute of Pathology, University Hospital Essen, University Duisburg-Essen, 45147 Essen, Germany
5
Senckenberg Institute for Pathology, Johann Wolfgang Goethe University Frankfurt, 60323 Frankfurt am Main, Germany
*
Author to whom correspondence should be addressed.
J. Fungi 2020, 6(4), 319; https://doi.org/10.3390/jof6040319
Received: 10 November 2020 / Revised: 25 November 2020 / Accepted: 25 November 2020 / Published: 27 November 2020
(This article belongs to the Special Issue Histoplasma and Histoplasmosis 2020)
Lack of sensitive diagnostic tests impairs the understanding of the epidemiology of histoplasmosis, a disease whose burden is estimated to be largely underrated. Broad-range PCRs have been applied to identify fungal agents from pathology blocks, but sensitivity is variable. In this study, we compared the results of a specific Histoplasma qPCR (H. qPCR) with the results of a broad-range qPCR (28S qPCR) on formalin-fixed, paraffin-embedded (FFPE) tissue specimens from patients with proven fungal infections (n = 67), histologically suggestive of histoplasmosis (n = 36) and other mycoses (n = 31). The clinical sensitivity for histoplasmosis of the H. qPCR and the 28S qPCR was 94% and 48.5%, respectively. Samples suggestive for other fungal infections were negative with the H. qPCR. The 28S qPCR did not amplify DNA of Histoplasma in FFPE in these samples, but could amplify DNA of Emergomyces (n = 1) and Paracoccidioides (n = 2) in three samples suggestive for histoplasmosis but negative in the H. qPCR. In conclusion, amplification of Histoplasma DNA from FFPE samples is more sensitive with the H. qPCR than with the 28S qPCR. However, the 28S qPCR identified DNA of other fungi in H. qPCR-negative samples presenting like histoplasmosis, suggesting that the combination of both assays may improve the diagnosis. View Full-Text
Keywords: Histoplasma qPCR; broad-range qPCR; formalin-fixed paraffin-embedded (FFPE) samples; histoplasmosis Histoplasma qPCR; broad-range qPCR; formalin-fixed paraffin-embedded (FFPE) samples; histoplasmosis
Show Figures

Figure 1

MDPI and ACS Style

Wilmes, D.; McCormick-Smith, I.; Lempp, C.; Mayer, U.; Schulze, A.B.; Theegarten, D.; Hartmann, S.; Rickerts, V. Detection of Histoplasma DNA from Tissue Blocks by a Specific and a Broad-Range Real-Time PCR: Tools to Elucidate the Epidemiology of Histoplasmosis. J. Fungi 2020, 6, 319. https://doi.org/10.3390/jof6040319

AMA Style

Wilmes D, McCormick-Smith I, Lempp C, Mayer U, Schulze AB, Theegarten D, Hartmann S, Rickerts V. Detection of Histoplasma DNA from Tissue Blocks by a Specific and a Broad-Range Real-Time PCR: Tools to Elucidate the Epidemiology of Histoplasmosis. Journal of Fungi. 2020; 6(4):319. https://doi.org/10.3390/jof6040319

Chicago/Turabian Style

Wilmes, Dunja; McCormick-Smith, Ilka; Lempp, Charlotte; Mayer, Ursula; Schulze, Arik B.; Theegarten, Dirk; Hartmann, Sylvia; Rickerts, Volker. 2020. "Detection of Histoplasma DNA from Tissue Blocks by a Specific and a Broad-Range Real-Time PCR: Tools to Elucidate the Epidemiology of Histoplasmosis" J. Fungi 6, no. 4: 319. https://doi.org/10.3390/jof6040319

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop