Next Article in Journal
Rezafungin—Mechanisms of Action, Susceptibility and Resistance: Similarities and Differences with the Other Echinocandins
Next Article in Special Issue
Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial
Previous Article in Journal
Nikkomycin Z—Ready to Meet the Promise?
Previous Article in Special Issue
Biotransformation of Animal Fat-By Products into ARA-Enriched Fermented Bioproducts by Solid-State Fermentation of Mortierella alpina
Open AccessArticle

Metal and Phosphate Ions Show Remarkable Influence on the Biomass Production and Lipid Accumulation in Oleaginous Mucor circinelloides

1
Faculty of Science and Technology, Norwegian University of Life Sciences, Drøbakveien 31, 1430 As, Norway
2
Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Christian Magnus Falsens vei 1, 1433 As, Norway
*
Author to whom correspondence should be addressed.
J. Fungi 2020, 6(4), 260; https://doi.org/10.3390/jof6040260
Received: 6 October 2020 / Revised: 24 October 2020 / Accepted: 27 October 2020 / Published: 30 October 2020
The biomass of Mucor circinelloides, a dimorphic oleaginous filamentous fungus, has a significant nutritional value and can be used for single cell oil production. Metal ions are micronutrients supporting fungal growth and metabolic activity of cellular processes. We investigated the effect of 140 different substrates, with varying amounts of metal and phosphate ions concentration, on the growth, cell chemistry, lipid accumulation, and lipid profile of M. circinelloides. A high-throughput set-up consisting of a Duetz microcultivation system coupled to Fourier transform infrared spectroscopy was utilized. Lipids were extracted by a modified Lewis method and analyzed using gas chromatography. It was observed that Mg and Zn ions were essential for the growth and metabolic activity of M. circinelloides. An increase in Fe ion concentration inhibited fungal growth, while higher concentrations of Cu, Co, and Zn ions enhanced the growth and lipid accumulation. Lack of Ca and Cu ions, as well as higher amounts of Zn and Mn ions, enhanced lipid accumulation in M. circinelloides. Generally, the fatty acid profile of M. circinelloides lipids was quite consistent, irrespective of media composition. Increasing the amount of Ca ions enhanced polyphosphates accumulation, while lack of it showed fall in polyphosphate. View Full-Text
Keywords: Mucor circinelloides; high-throughput screening; metal ions; phosphorus; lipids; biofuel; FTIR spectroscopy; bioremediation; co-production Mucor circinelloides; high-throughput screening; metal ions; phosphorus; lipids; biofuel; FTIR spectroscopy; bioremediation; co-production
Show Figures

Figure 1

MDPI and ACS Style

Dzurendova, S.; Zimmermann, B.; Tafintseva, V.; Kohler, A.; Horn, S.J.; Shapaval, V. Metal and Phosphate Ions Show Remarkable Influence on the Biomass Production and Lipid Accumulation in Oleaginous Mucor circinelloides. J. Fungi 2020, 6, 260. https://doi.org/10.3390/jof6040260

AMA Style

Dzurendova S, Zimmermann B, Tafintseva V, Kohler A, Horn SJ, Shapaval V. Metal and Phosphate Ions Show Remarkable Influence on the Biomass Production and Lipid Accumulation in Oleaginous Mucor circinelloides. Journal of Fungi. 2020; 6(4):260. https://doi.org/10.3390/jof6040260

Chicago/Turabian Style

Dzurendova, Simona; Zimmermann, Boris; Tafintseva, Valeria; Kohler, Achim; Horn, Svein J.; Shapaval, Volha. 2020. "Metal and Phosphate Ions Show Remarkable Influence on the Biomass Production and Lipid Accumulation in Oleaginous Mucor circinelloides" J. Fungi 6, no. 4: 260. https://doi.org/10.3390/jof6040260

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop