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Consensus Multilocus Sequence Typing Scheme for Pneumocystis jirovecii

Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Clinical School, Sydney Medical School, Faculty of Medicine and Health, The University of Sydney, Sydney 2006, Australia
Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, Sydney 2006, Australia
Westmead Institute for Medical Research, Westmead 2145, Australia
Microbiology Department, Hospital Universitari Vall d’Hebron (VHUH), 08035 Barcelona, Spain
Genetics and Microbiology Department, Universitat Autònoma de Barcelona (UAB), 08035 Barcelona, Spain
Instituto de Ciencias Biomédicas (ICBM), Facultad de Medicina, Universidad de Chile, Santiago de Chile 8380453, Chile
Westmead Hospital (Research and Education Network), Westmead 2145, Australia
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
J. Fungi 2020, 6(4), 259;
Received: 10 September 2020 / Revised: 10 October 2020 / Accepted: 18 October 2020 / Published: 30 October 2020
(This article belongs to the Special Issue Epidemiology, Diagnosis of Fungal Infections)
Pneumocystis jirovecii is an opportunistic human pathogenic fungus causing severe pneumonia mainly in immunocompromised hosts. Multilocus sequence typing (MLST) remains the gold standard for genotyping of this unculturable fungus. However, the lack of a consensus scheme impedes a global comparison, large scale population studies and the development of a global MLST database. To overcome this problem this study compared all genetic regions (19 loci) currently used in 31 different published Pneumocystis MLST schemes. The most diverse/commonly used eight loci, β-TUB, CYB, DHPS, ITS1, ITS1/2, mt26S and SOD, were further assess for their ability to be successfully amplified and sequenced, and for their discriminatory power. The most successful loci were tested to identify genetically related and unrelated cases. A new consensus MLST scheme consisting of four genetically independent loci: β-TUB, CYB, mt26S and SOD, is herein proposed for standardised P. jirovecii typing, successfully amplifying low and high fungal burden specimens, showing adequate discriminatory power, and correctly identifying suspected related and unrelated isolates. The new consensus MLST scheme, if accepted, will for the first time provide a powerful tool to investigate outbreak settings and undertake global epidemiological studies shedding light on the spread of this important human fungal pathogen. View Full-Text
Keywords: P. jirovecii; consensus MLST scheme; β-TUB; CYB; mt26S and SOD P. jirovecii; consensus MLST scheme; β-TUB; CYB; mt26S and SOD
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MDPI and ACS Style

Pasic, L.; Goterris, L.; Guerrero-Murillo, M.; Irinyi, L.; Kan, A.; Ponce, C.A.; Vargas, S.L.; Martin-Gomez, M.T.; Meyer, W. Consensus Multilocus Sequence Typing Scheme for Pneumocystis jirovecii. J. Fungi 2020, 6, 259.

AMA Style

Pasic L, Goterris L, Guerrero-Murillo M, Irinyi L, Kan A, Ponce CA, Vargas SL, Martin-Gomez MT, Meyer W. Consensus Multilocus Sequence Typing Scheme for Pneumocystis jirovecii. Journal of Fungi. 2020; 6(4):259.

Chicago/Turabian Style

Pasic, Lana, Lidia Goterris, Mercedes Guerrero-Murillo, Laszlo Irinyi, Alex Kan, Carolina A. Ponce, Sergio L. Vargas, M. T. Martin-Gomez, and Wieland Meyer. 2020. "Consensus Multilocus Sequence Typing Scheme for Pneumocystis jirovecii" Journal of Fungi 6, no. 4: 259.

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