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Open AccessArticle

Identification of Mucorales by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

1
Department of Internal Medicine, Respiratory and Critical Care Medicine, University Hospital Marburg, D-35043 Marburg, Germany
2
Center for Invasive Mycoses and Antifungals, Philipps University Marburg, D-35037 Marburg, Germany
3
Université Paris Descartes, Faculté de Médecine, AP-HP, Hôpital Européen Georges Pompidou, Unité de Parasitologie-Mycologie, F-75015 Paris, France
4
Centre Hospitalier Régional Universitaire, Hôpital Jean Minjoz, Sérologies Parasitaires et Fongiques, F-25030 Besançon, France
5
Working Group Dynamyc, Faculté de Médecine, Hôpital Henri Mondor, F-94010 Créteil, France
*
Author to whom correspondence should be addressed.
J. Fungi 2019, 5(3), 56; https://doi.org/10.3390/jof5030056
Received: 22 May 2019 / Revised: 13 June 2019 / Accepted: 28 June 2019 / Published: 2 July 2019
(This article belongs to the Special Issue Mucorales and Mucormycosis)
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Abstract

More than 20 different species of Mucorales can be responsible for human mucormycosis. Accurate identification to the species level is important. The morphological identification of Mucorales is not reliable, and the currently recommended identification standard is the molecular technique of sequencing the internal transcribed spacer regions. Nevertheless, matrix-assisted laser desorption ionization time-of-flight mass spectrometry has been shown to be an accurate alternative for the identification of bacteria, yeasts, and even filamentous fungi. Therefore, 38 Mucorales isolates, belonging to 12 different species or varieties, mainly from international collections, including 10 type or neo-type strains previously identified by molecular methods, were used to evaluate the usefulness of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the identification of human pathogenic Mucorales to the species level. One to three reference strains for each species were used to create a database of main spectrum profiles, and the remaining isolates were used as test isolates. A minimum of 10 spectra was used to build the main spectrum profile of each database strain. Interspecies discrimination for all the isolates, including species belonging to the same genus, was possible. Twenty isolates belonging to five species were used to test the database accuracy, and were correctly identified to the species level with a log-score >2. In summary, matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a reliable and rapid method for the identification of most of the human pathogenic Mucorales to the species level. View Full-Text
Keywords: mucorales; mucormycosis; identification; MALDI-TOF mass spectrometry mucorales; mucormycosis; identification; MALDI-TOF mass spectrometry
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Schwarz, P.; Guedouar, H.; Laouiti, F.; Grenouillet, F.; Dannaoui, E. Identification of Mucorales by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry. J. Fungi 2019, 5, 56.

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