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Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus

1
Unité des Aspergillus, 25 rue du Docteur Roux, Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, France
2
Unité de Biologie des ARN des Pathogènes Fongiques, Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, France
3
Unité de Spectrométrie de Masse pour la Biologie, Institut Pasteur, CNRS USR 2000, 28 rue du Docteur Roux, 75015 Paris, France
*
Author to whom correspondence should be addressed.
J. Fungi 2018, 4(1), 19; https://doi.org/10.3390/jof4010019
Received: 8 December 2017 / Revised: 12 January 2018 / Accepted: 19 January 2018 / Published: 2 February 2018
(This article belongs to the Special Issue Fungal Cell Wall)
Glycosylphosphatidylinositols (GPIs) are lipid anchors allowing the exposure of proteins at the outer layer of the plasma membrane. In fungi, a number of GPI-anchored proteins (GPI-APs) are involved in the remodeling of the cell wall polymers. GPIs follow a specific biosynthetic pathway in the endoplasmic reticulum. After the transfer of the protein onto the GPI-anchor, a lipid remodeling occurs to substitute the diacylglycerol moiety by a ceramide. In addition to GPI-APs, A. fumigatus produces a GPI-anchored polysaccharide, the galactomannan (GM), that remains unique in the fungal kingdom. To investigate the role of the GPI pathway in the biosynthesis of the GM and cell wall organization, the deletion of PER1—coding for a phospholipase required for the first step of the GPI lipid remodeling—was undertaken. Biochemical characterization of the GPI-anchor isolated from GPI-APs showed that the PER1 deficient mutant produced a lipid anchor with a diacylglycerol. The absence of a ceramide on GPI-anchors in the Δper1 mutant led to a mislocation of GPI-APs and to an alteration of the composition of the cell wall alkali-insoluble fraction. On the other hand, the GM isolated from the Δper1 mutant membranes possesses a ceramide moiety as the parental strain, showing that GPI anchor of the GM follow a distinct unknown biosynthetic pathway. View Full-Text
Keywords: glycosylphosphatidylinositol; Aspergillus fumigatus; PER1; cell wall; galactomannan glycosylphosphatidylinositol; Aspergillus fumigatus; PER1; cell wall; galactomannan
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Li, J.; Mouyna, I.; Henry, C.; Moyrand, F.; Malosse, C.; Chamot-Rooke, J.; Janbon, G.; Latgé, J.-P.; Fontaine, T. Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus. J. Fungi 2018, 4, 19.

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