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Perstraction of Intracellular Pigments through Submerged Fermentation of Talaromyces spp. in a Surfactant Rich Media: A Novel Approach for Enhanced Pigment Recovery

1
School of Engineering, University College Cork, Cork, Ireland
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Department of Chemical Engineering, Universidad Autónoma de Coahuila, Saltillo 25280, Mexico
*
Author to whom correspondence should be addressed.
J. Fungi 2017, 3(3), 33; https://doi.org/10.3390/jof3030033
Received: 1 June 2017 / Revised: 21 June 2017 / Accepted: 22 June 2017 / Published: 27 June 2017
(This article belongs to the Special Issue Fungal Pigments)
A high percentage of the pigments produced by Talaromyces spp. remains inside the cell, which could lead to a high product concentration inhibition. To overcome this issue an extractive fermentation process, perstraction, was suggested, which involves the extraction of the intracellular products out of the cell by using a two-phase system during the fermentation. The present work studied the effect of various surfactants on secretion of intracellular pigments produced by Talaromyces spp. in submerged fermentation. Surfactants used were: non-ionic surfactants (Tween 80, Span 20 and Triton X-100) and a polyethylene glycerol polymer 8000, at different concentrations (5, 20, 35 g/L). The highest extracellular pigment yield (16 OD500nm) was reached using Triton X-100 (35 g/L), which was 44% higher than the control (no surfactant added). The effect of addition time of the selected surfactant was further studied. The highest extracellular pigment concentration (22 OD500nm) was achieved when the surfactant was added at 120 h of fermentation. Kinetics of extracellular and intracellular pigments were examined. Total pigment at the end of the fermentation using Triton X-100 was 27.7% higher than the control, confirming that the use of surfactants partially alleviated the product inhibition during the pigment production culture. View Full-Text
Keywords: fungal pigments; perstraction; surfactant; Talaromyces fungal pigments; perstraction; surfactant; Talaromyces
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Morales-Oyervides, L.; Oliveira, J.; Sousa-Gallagher, M.; Méndez-Zavala, A.; Montañez, J.C. Perstraction of Intracellular Pigments through Submerged Fermentation of Talaromyces spp. in a Surfactant Rich Media: A Novel Approach for Enhanced Pigment Recovery. J. Fungi 2017, 3, 33.

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