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Volume 12
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10.3390/jof12090698
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Multimodal Analysis and Heterologous Expression Strategies Reveal the Crucial Role of the Lac2 Gene from Lentinus sajor-caju in Lignin Degradation

by
Changlong Gou
1,
Lilong Luo
1,
Liang Zhang
1,
Liming Chen
1,
Yangci Liao
2 and
Yuqiong Wang
1,*
1
College of Animal Science and Technology, Inner Mongolia Minzu University, 996 West Lamoulon Street, Tongliao 028000, China
2
Institute of Pratacultural, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China
*
Author to whom correspondence should be addressed.
J. Fungi 2026, 12(9), 698; https://doi.org/10.3390/jof12090698 (registering DOI)
Submission received: 24 July 2026 / Revised: 7 September 2026 / Accepted: 15 September 2026 / Published: 17 September 2026
(This article belongs to the Special Issue Molecular Biology of Mushroom, 2nd Edition)
Versions Notes

Abstract

The complex chemical structure of plant lignin limits roughage utilization. Biological modification is commonly used to enhance lignin properties. Lentinus sajor-caju (L. sajor-caju) is a potent white-rot fungus for lignocellulose pretreatment in animal roughage. However, the key lignin-degrading enzyme genes remain unclear. This study evaluated the key enzyme genes involved in lignin degradation and their efficiency in decomposing alkaline lignin. Transcriptome and proteome comparative analyses of L. sajor-caju cultured in media with glucose and alkaline lignin as carbon sources revealed that the AA1 laccase aids lignin decomposition in L. sajor-caju. Additionally, a laccase heterologously expressing strain in Pichia pastoris X-33 was constructed. Compared with the wild-type L. sajor-caju strain W-2, Lac2 laccase activity increased by 67.35%. Electron microscopy showed that Lac2 degraded alkaline lignin, forming irregular pores and surface fusion. These findings indicate that Lac2 laccase has potential application value. In the future, it may be applied in agriculture, the chemical industry, and the energy sector to efficiently convert lignin into feed additives or energy products.
Keywords: Lentinus sajor-caju; alkaline lignin; Lac2; overexpression Lentinus sajor-caju; alkaline lignin; Lac2; overexpression

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MDPI and ACS Style

Gou, C.; Luo, L.; Zhang, L.; Chen, L.; Liao, Y.; Wang, Y. Multimodal Analysis and Heterologous Expression Strategies Reveal the Crucial Role of the Lac2 Gene from Lentinus sajor-caju in Lignin Degradation. J. Fungi 2026, 12, 698. https://doi.org/10.3390/jof12090698

AMA Style

Gou C, Luo L, Zhang L, Chen L, Liao Y, Wang Y. Multimodal Analysis and Heterologous Expression Strategies Reveal the Crucial Role of the Lac2 Gene from Lentinus sajor-caju in Lignin Degradation. Journal of Fungi. 2026; 12(9):698. https://doi.org/10.3390/jof12090698

Chicago/Turabian Style

Gou, Changlong, Lilong Luo, Liang Zhang, Liming Chen, Yangci Liao, and Yuqiong Wang. 2026. "Multimodal Analysis and Heterologous Expression Strategies Reveal the Crucial Role of the Lac2 Gene from Lentinus sajor-caju in Lignin Degradation" Journal of Fungi 12, no. 9: 698. https://doi.org/10.3390/jof12090698

APA Style

Gou, C., Luo, L., Zhang, L., Chen, L., Liao, Y., & Wang, Y. (2026). Multimodal Analysis and Heterologous Expression Strategies Reveal the Crucial Role of the Lac2 Gene from Lentinus sajor-caju in Lignin Degradation. Journal of Fungi, 12(9), 698. https://doi.org/10.3390/jof12090698

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