Advancing Dermatomycosis Diagnosis: Evaluating a Microarray-Based Platform for Rapid and Accurate Fungal Detection—A Pilot Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Samples
2.2. Standard Mycological Assays
2.3. EUROArray Dermatomycosis Platform Assay
2.4. Analysis of Results
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strains from Indicated Species (No. of Tested) | Results by Strains, Expressed as |
---|---|
No. of Identified | |
On-panel species | |
Candida albicans (2) | 2 |
Candida parapsilosis (3) | 3 |
Fusarium oxysporum (2) | 2 |
Fusarium solani (3) | 2 a |
Microsporum audouinii (1) | 1 |
Microsporum canis (2) | 2 |
Microsporum gypseum (2) | 2 |
Nannizia fulva (1) | 1 |
Trichophyton erinacei (2) | 2 |
Trichophyton interdigitale (6) | 6 |
Trichophyton mentagrophytes (5) | 5 |
Trichophyton rubrum (3) | 3 |
Trichophyton tonsurans (1) | 1 |
Trichophyton violaceum (1) | 1 |
Total species (34) | 33 |
Off-panel species | |
Aspergillus fumigatus (1) | 0 |
Fusarium delphinoides (1) | 0 |
Penicillium chrysogenum (2) | 0 |
Trichophyton indotineae (2) | Both identifed as T. mentagrophytes |
Total species (6) | 0 |
Fungal Species | Identified by the EUROArray Platform (No. of Positive Samples) | Identified by Culture (No. of Positive Samples) |
---|---|---|
Dermatophyte (universal) | 1 | Not applicable |
Alternaria alternata | 0 a | 4 |
Aspergillus niger | 0 a | 1 |
Candida albicans | 1 | 2 |
Candida parapsilosis | 11 | 5 |
Candida guilliermondii | 1 | 1 |
Fusarium solani | 2 | 3 |
Nannizia gypsea | 2 | 0 |
Rhizopus arrhizus | 0 a | 1 |
Rhodotorula mucilaginosa | 0 a | 1 |
Trichophyton interdigitale | 2 | 1 |
Trichophyton indotinae | 1 b | 1 |
Trichosporon mucoides | 0 a | 1 |
Trichophyton rubrum | 9 | 8 |
EUROArray Results Evaluated According to Those from the Comparator (Microscopy and/or Culture) a | |
---|---|
Metric | Value |
True positives (n) | 22 |
False negatives (n) | 6 |
False positives (n) | 7 |
True negatives (n) | 77 |
Results evaluated in total (n) | 112 |
Sensitivity (%) | 78.6 |
Specificity (%) | 91.7 |
Positive predictive value (%) | 75.9 |
Negative predictive value (%) | 92.8 |
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Ivagnes, V.; De Carolis, E.; Magrì, C.; Torelli, R.; Posteraro, B.; Sanguinetti, M. Advancing Dermatomycosis Diagnosis: Evaluating a Microarray-Based Platform for Rapid and Accurate Fungal Detection—A Pilot Study. J. Fungi 2025, 11, 234. https://doi.org/10.3390/jof11030234
Ivagnes V, De Carolis E, Magrì C, Torelli R, Posteraro B, Sanguinetti M. Advancing Dermatomycosis Diagnosis: Evaluating a Microarray-Based Platform for Rapid and Accurate Fungal Detection—A Pilot Study. Journal of Fungi. 2025; 11(3):234. https://doi.org/10.3390/jof11030234
Chicago/Turabian StyleIvagnes, Vittorio, Elena De Carolis, Carlotta Magrì, Riccardo Torelli, Brunella Posteraro, and Maurizio Sanguinetti. 2025. "Advancing Dermatomycosis Diagnosis: Evaluating a Microarray-Based Platform for Rapid and Accurate Fungal Detection—A Pilot Study" Journal of Fungi 11, no. 3: 234. https://doi.org/10.3390/jof11030234
APA StyleIvagnes, V., De Carolis, E., Magrì, C., Torelli, R., Posteraro, B., & Sanguinetti, M. (2025). Advancing Dermatomycosis Diagnosis: Evaluating a Microarray-Based Platform for Rapid and Accurate Fungal Detection—A Pilot Study. Journal of Fungi, 11(3), 234. https://doi.org/10.3390/jof11030234