Three New Truffle Species (Tuber, Tuberaceae, Pezizales, and Ascomycota) from Yunnan, China, and Multigen Phylogenetic Arrangement within the Melanosporum Group
Abstract
1. Introduction
2. Materials and Methods
2.1. Morphological Study
2.2. Molecular Methods
2.3. Sequence Analysis
Cavity | Fungal Taxa | Voucher Specimens | Ascomata Surface | Asci Spore Number | Ascospores | Source | |||
---|---|---|---|---|---|---|---|---|---|
Color | Warts | Q Interval of All Spores, Average Value of All Spores and Main Shape | Size (μm) | Ornamentation | |||||
Yes | Tuber brumale | — | black | penta- or hexagonal flat warts | 3–5 | nd Qm = 1.6 ± 0.1, mainly long ellipsoid | (25.3)–28.1–(33.7) × (15.7)–17.4–(19.1) μm | 3.4 ± 0.38 μm, spino-reticulate | Vittadini (1831) [22] Wang et al. (2006) [34] |
Tuber pseudohimalayense | AH 18331 | black | Pyramidal | 1–7(8) | nd Qm = 1.30 ± 0.09, mainly broadly ellipsoidal, | 1-spored: 34–35 × 25–30 μm 2-spored: 30–34 × 22–30 μm 3–7-spored: — (including the ornamentation) | 4–6(–8) μm, spino-reticulate | Moreno et al. (1997) [36] Wang et al. (2006) [34] Chen & Liu (2011) [33] | |
Tuber melanoumbilicatum | YNAU017 holotype | black | solid, irregular polygonal, pyramidal warts | (1–)2–7 | Q = 1.2–2.0, Qm = 1.65 ± 0.1, mainly long ellipsoid | 1-spored: (42.5–)44.6–48.8(–54.5) × (25.7–)26.6–29.1(–30.2) μm 2-spored: (26.3–)30.9–41.4(–42.2) × (18.2–)18.5–23.9(–24.6) μm 3-spored: (19.2–)20.9–35.4(-36.9) × (13.7–)15.4–22.2(–26.2) μm 4-spored: (21.5–)24.3–31.7(–32.2) – (14.2–)14.9–20.2(–21.4) μm 5-spored: (21.7–)22.2–29.9(–33.1) × (13.1–)14.2–18.1(–20.2) μm 6-spored: (19.2–)21.7–28.0(–29.4) × (11.7)13.1–17.5(–18.9) μm 7-spored: (16.8–)18.9–26.8(–27.4) × (8.8–)12.5–16.3(–16.8) μm | 0.9–8.2 μm, spino-reticulate | This study | |
Tuber pseudobrumale | YAAS L3181 holotype | black | low pyramidal warts | 3–7 | nd Q = 1.27, mainly ellipsoid | 3-spored: 26–30 × 15.5–17.5 μm 4-spored: (22)23–25.5(27) × 14–17 μm 5-spored: (21)22–25(25.4) × 13.5–15(16) μm 6-spored: 21–23.5 × 13–14.5 μm 7-spored: 21–22.5 × 12–14 μm | 4–5 μm spino-reticulate | Li et al. (2014) [41] | |
Tuber melanoexcavatum | YAAS L3605 holotype | black | pyramidal warts | 5–8 | nd Q = 1.19, mainly ellipsoid, | 5-spored: 22–24.7 × 15.4–16.9 μm 6-spored: 21.4–24 × 14.3–16.0 μm 7-spored: 20–22 × 13.5–15.5 μm 8-spored: 18.7–21.2 × 12.6–15.1 μm | 3–4 μm spino-reticulate | Wang et al. (2020) [42] | |
Tuber pseudobrumale | (22 samples shown on Table A1) | black | solid, low, concave, irregular, polygonal warts | 1–6(–8) | Q = 1.1–1.9, Qm = 1.45 ± 0.2, malily ellipsoid | 1-spored: (19.1–)22.4–42.2(–52.3) × (13.8–)16.2–27.8(–36.7) μm 2-spored: (17.5–)21.8–36.3(–42.5) × (12.6–)16.1–23.9(–31.9) μm 3-spored: (13.8–)20.1–26.1(–40.6) × (12.4–)14.7–21.8(–29.0) μm 4-spored: (13.3–)17.9–28.3(–33.9) × (9.6–)13.5–19.3(–26.1) μm 5-spored: (13.0–)17.3–26.5(–32.0) × (9.6–)12.6–18.4(–22.8) μm 6-spored: (11.5–)16.7–25.4(–33.5) × (9.1–)12.3–18.2(–23.3) μm 7-spored: (9.6–)12.8–23.7(–28.4) × (5.6–)9.6–16.1(–18.1) μm 8-spored: (15.9–)18.2–21.9(–22.7) × (10.5–)12.4–15.1(–15.7) μm | 0.4–9.6 μm, spino-reticulate | This study | |
Tuber variabilisporum | BJTC FAN362 holotype | dark brown to black brown | verrucose | 1–5(–6) | Q = 1.06–1.44, nd broadly ellipsoid and ellipsoid | 1-spored: 30–37.5 × 21.75–27.5 μm 2-spored: 27.5–32.5 ×20–23.5 μm 3-spored: 22.5–32.5 × 18.75–21.25 μm 4-spored: 17.5–25.5 ×16.5–20 μm 5-spored: 16.5–22.5 × 13.25–17.5 μm | 3–5 μm, spino-reticulate | Fan et al. (2022) [6] | |
Tuber microexcavatum | YNAU 1263 holotype | yellowish brown | loose-textured, cracked irregular warts | 1–6 | nd nd ellipsoid | nd | nd | This study | |
Tuber sp. 5 | K229 | black | pyramidal warts | 5–8 | Q = 1.0–2.0, nd ellipsoid | 15–20 × 10–15 μm | nd spino-reticulate | Kinoshita et al. (2011) [17] | |
No | Tuber longispinosum | K447 holotype | brown to dark greyish | low polygonal warts | 1–5(–6) | Q = 1.0–2.1, nd ellipsoid to subglobose | 1-spored: 31–41 × 22–30 μm 2-spored: 21–38 × 16–29 μm 3-spored: 19–34 × 15–26 μm 4-spored: 15–33 × 13–22 μm 5-spored: 16–31 × 12–20 μm 6-spored: 15–26 × 13–18 μm | 3–7 (–12) μm, spiny | Kinoshita et al. (2018) [32] |
Tuber yunnanense | YNAU019 holotype | dark brown to black | solid, irregular polygonal, clustered pyramidal ridged warts | 1–5(–6) | Q = 1.1–2.2, Qm = 1.74 ± 0.1, malily long shuttle-shaped | 1-spored: (31.8–)32.3–52.8(–54.6) × (19.1–)20.2–33.1(–35.4) μm 2-spored: (25.4–)30.9–39.8(–43.6) × (15.7–)17.1–22.7(–25.1) μm 3-spored: (26.7–)28.1–35.9(–43.3) × (14.7–)15.4–21.4(–29.4) μm 4-spored: (25.1–)26.1–31.6(–32.7) × (14.3–)14.7–18.3(–20.6) μm 5-spored: (20.0–)20.6–28.2(–28.6) × (11.6–)12.8–17.1(–18.3) μm 6-spored: (22.5–)23.1–28.8(–29.4) × (11.7–)14.0–18.6(–19.2) μm | 0.7–11.1 μm, spiny | This study | |
Tuber regimontanum | ITCV 909 holotype | dark brown to black | pyramidal verrucae | 1–4 | nd nd broadly fusiform to ellipsoid. | 1-spore: 40–55 (–62) × 30–31 μm 2-spore: 37–42 × 25–26 μm 3-spore: 33–37 × 23–26 μm 4-spore: 28–35 × 18–22 μm | 2–5 × 1–2 µm, spino-reticulate | Guevara et al. (2008) [40] | |
Tuber yigongense | BJTC FAN731 holotype | dark brown to blackish | pentagonal and pyramidal warts | 1–5 | nd nd malily ellipsoid | 1-spored: 35–45 × 25–30 μm 2-spored: 30–37.5 × 20–25 μm 3–5-spored: 20–32.5 × 17.5–22.5 μm | 2.5–4 µm, densely spino-reticulate | Fan et al. (2018) [35] | |
Tuber sinense= T. indicum? | MHSU 1633 | brown, reddish brown or deeply brown | verrucose | 1–4 | nd nd malily ellipsoid | 1-spore: 32–36.5 × 43–49.5 μm 2-spores: 26.5–30 × 39–45.5 μm 3-4-spores: 22.5–25 × 30–35 μm (including spines) | 3–6(–7) µm, spiny | Tao et al. (1989) [26] Wang et al. (2006) [34] Chen (2007) [47] Fan et al. (2022) [6] | |
Tuber formosanum | HKAS 62628 | dark reddish brown to dark grayish brown | low pyramidal warts | 1–4(–5) | Q = (1.17–)1.27–1.62(–1.70) nd malily ellipsoid | 1-spored: (27–)29–45(–48) × 20–32(–35) μm 2-spored: (26–)27–36(–39) × (18–)19–24(–28) μm 3-spored: 24–34 × (16–)18–23(–25) μm 4-spored:(25–)26–32(–33) × (17–)18–22 μm | 2–5(–6) µm, spino-reticulate | Qiao et al. (2013) [28] | |
Tuber melanosporum | — | blackish | verrucose | 1–5 | Q = 1.4–2.1 nd malily ellipsoid | 28–32 × 16–21 μm | 2–4 μm.spiny, spino-reticulate | Vittadini (1831) [22] Wang et al. (2006) [34] |
3. Results
3.1. Phylogenetic Analysis
3.2. Taxonomy
3.2.1. Tuber yunnanense S. P. Wan, R. Wang and F.Q. Yu, sp. nov. Figure 2
3.2.2. Tuber melanoumbilicatum S. P. Wan, R. Wang, and F.Q. Yu, sp. nov. Figure 3
3.2.3. Tuber microexcavatum S. P. Wan, R. Wang, and F.Q. Yu, sp. nov. Figure 4
3.2.4. Tuber pseudobrumale Y. Wang and Shu H. Li, Mycol. Prog. 2014, 13, 1157–1163 (Figure 5)
3.2.5. Tuber variabilisporum L. Fan and T. Li, Persoonia-Molecular Phylogeny and Evolution of Fungi. 2022, 48(1): 175–202 (Figure 6)
3.2.6. Tuber pseudohimalayense Moreno G, Díez M and García-Moreno, Mycotaxon. 1997, 63: 217–224 (Figure 6)
4. Discussion
1. Ascomata has a cavity | 2 |
1. Ascomata no a cavity | 3 |
2. Ascomata yellowish brown | T. microexcavatum |
2. Ascomata black | 4 |
3. Spore shape, Q > 1.7 | 5 |
3. Spore shape, Q < 1.7 | 7 |
4. Spore shape, broadly ellipsoidal or ellipsoidal | 6 |
4. Spore shape, long ellipsoid | 8 |
5. Spore shape, mainly long shuttle-shaped | T. yunnanense |
5. Spore shape, mainly ellipsoid | 9 |
6. Spore shape, broadly ellipsoidal | T. pseudohimalayense |
6. Spore shape, mainly ellipsoid | 10 |
7. Height of spore ornamentation, 3–6(–7) µm, spiny | T. sinense |
7. Height of spore ornamentation, 2–5(–6) µm, spino-reticulate | T. formosanum |
8. Height of spore ornamentation, 3.4 ± 0.38 µm, spino-reticulate | T. brumale |
8. Height of spore ornamentation, 0.9–8.2 µm, spino-reticulate | T. melanoumbilicatum |
9. Height of spore ornamentation, spiny | T. longispinosum |
9. Height of spore ornamentation, spino-reticulate | 11 |
10. Height of spore ornamentation, 0.4–9.6 µm, spino-reticulate | T. pseudobrumale |
10. Height of spore ornamentation, spino-reticulate | 12 |
11. Height of spore ornamentation, 2.5–4 µm, densely spino-reticulate | T. yigongense |
11. Height of spore ornamentation, spino-reticulate | 13 |
12. Spore length and width, 15–20 × 10–15 μm | Tuber sp. 5 |
12. Spore length and width, 16.5–37.5 × 13.25–27.5 μm | T. variabilisporum |
13. Spore length and width, 28–55 × 18–31 μm | T. regimontanum |
13. Spore length and width, 28–32 × 16–21 μm | T. melanosporum |
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Taxon Name | Lineage | Voucher Specimen | Country | ITS | LSU | tef1-α | rpb2 |
---|---|---|---|---|---|---|---|
Choiromyces sichuanensis | outgroup | YNAU003, holotype | Sichuan, China | MW380902 | OK576632 | PP092037 | OR832439 |
Choiromyces sichuanensis | outgroup | YNAU004 | Sichuan, China | OK585070 | OK576633 | PP092038 | OR832440 |
Tuber aestivum | aestivum | GB202 | Italy | – | JQ925679 | JX022565 | JQ954487 |
Tuber aestivum | aestivum | JT30500 | Sweden | HM485340 | – | – | JQ954488 |
Tuber borchii | puberulum | BJTC FAN217 | New Zealand | KT067681 | KT067706 | KT067717 | OM584229 |
Tuber borchii | puberulum | GB1/GB32 | Italy | FJ809852 | FJ809799 | JX022571 | JQ954492 |
Tuber brumale | melanosporum | GB52 | Italy | HM485345 | JQ925683 | – | JQ954494 |
Tuber brumale | melanosporum | GB53 | Italy | FJ748900 | JQ925684 | – | JQ954495 |
Tuber californicum | puberulum | JT28058 | USA | HM485346 | JQ925685 | JX022574 | JQ954496 |
Tuber canaliculatum | macrosporum | JT28215 | USA | JQ925643 | – | JX022575 | JQ954497 |
Tuber canaliculatum | macrosporum | OSC59072 | USA | HM485347 | – | JX022576 | JQ954498 |
Tuber crassitunicatum | rufum | BJTC FAN465, holotype | Yunnan, China | MH115295 | OM366205 | OM649610 | OM584268 |
Tuber depressum | excavatum | BJTC FAN340 | Sichuan, China | OM256744 | OM366187 | OM649592 | OM584250 |
Tuber depressum | excavatum | BJTC FAN534 | Sichuan, China | OM256764 | OM366211 | OM649616 | – |
Tuber dryophilum | puberulum | GB35 | Italy | JQ925644 | JQ925687 | JX022577 | – |
Tuber dryophilum | puberulum | GB37 | Italy | HM485354 | JQ925688 | JX022578 | JQ954501 |
Tuber formosanum | melanosporum | BJTC FAN107 | Yunnan, China | MF621549 | OM366159 | OM649564 | OM584210 |
Tuber formosanum | melanosporum | BJTC FAN356 | Sichuan, China | MF627986 | OM366189 | OM649594 | OM584252 |
Tuber glabrum | macrosporum | BJTC FAN228, holotype | Yunnan, China | KF002731 | OM366177 | OM649581 | OM584234 |
Tuber glabrum | macrosporum | BJTC FAN232, paratype | Yunnan, China | KF002727 | OM366179 | OM649583 | OM584236 |
Tuber huidongense | rufum | BJTC FAN104 | Yunnan, China | JF921163 | OM366158 | OM649563 | OM584209 |
Tuber huidongense | rufum | BJTC FAN101 | Yunnan, China | OM311172 | OM366156 | OM649562 | OM584208 |
Tuber jinshajiangense | puberulum | BJTC FAN406 | Yunnan, China | KX575841 | OM366199 | OM649604 | OM584262 |
Tuber jinshajiangense | puberulum | BJTC FAN407 | Yunnan, China | KX575842 | OM366200 | OM649605 | OM584263 |
Tuber liaotongense | rufum | BJTC FAN550 | Beijing, China | MH115302 | OM366213 | OM649618 | OM584272 |
Tuber lijiangense | puberulum | BJTC FAN307 | Yunnan, China | KP276188 | KP276203 | KP276206 | OM584244 |
Tuber lishanense | rufum | BJTC FAN718, holotype | Shanxi, China | MH115303 | MH115304 | OM649622 | OM584276 |
Tuber lishanense | rufum | BJTCFAN683 | Shanxi, China | MH115305 | MH115306 | OM649621 | OM584275 |
Tuber longispinosum | melanosporum | K225 | Japan | AB553414 | AB553518 | AB553538 | AB553558 |
Tuber longispinosum | melanosporum | K447, holotype | Japan | AB553423 | – | – | – |
Tuber macrosporum | macrosporum | JT13362 | Italy | HM485373 | FJ809838 | JX022590 | – |
Tuber magnatum | aestivum | GB12 | Italy | JQ925645 | JQ925700 | JX022591 | JQ954512 |
Tuber magnatum | aestivum | GB13 | Italy | JQ925646 | JQ925701 | JX022592 | JQ954513 |
Tuber melanoexcavatum | melanosporum | YAAS L3605, holotype | Yunnan, China | KY081684 | – | – | – |
Tuber melanoexcavatum | melanosporum | YAAS L3636 | Yunnan, China | KY081685 | – | – | – |
Tuber melanosporum | melanosporum | GB200 | Italy | FJ748904 | JQ925703 | JX022594 | JQ954515 |
Tuber melanoumbilicatum | melanosporum | YNAU017, holotype | Yunnan, China | OK625304 | OR661815 | OR832379 | OR832411 |
Tuber melanoumbilicatum | melanosporum | YNAU018 | Yunnan, China | OK625305 | OR661816 | OR832380 | OR832412 |
Tuber microexcavatum | melanosporum | YNAU1263 | Yunnan, China | OR250184 | OR661838 | OR832381 | OR832413 |
Tuber microexcavatum | melanosporum | YNAU1264 | Yunnan, China | OR250185 | OR661839 | OR832382 | OR832414 |
Tuber neoexcavatum | excavatum | BJTC FAN184, holotype | Yunnan, China | JX458715 | OM366169 | OM649574 | – |
Tuber neoexcavatum | excavatum | BJTC FAN316 | Yunnan, China | OM256741 | OM366184 | OM649589 | OM584247 |
Tuber oligospermum | puberulum | AH38984 | Spain | JN392261 | JN392320 | – | – |
Tuber oligospermum | puberulum | AH37783 | Spain | JN392260 | JN392324 | – | – |
Tuber pseudobrumale | melanosporum | YAAS L3181, holotype | Yunnan, China | KJ742703 | – | – | – |
Tuber pseudobrumale | melanosporum | BJTC FAN306 | Yunnan, China | OM287838 | OM366183 | OM649587 | OM584243 |
Tuber pseudobrumale | melanosporum | BJTC FAN322 | Yunnan, China | OM287839 | OM366186 | OM649591 | OM584249 |
Tuber pseudobrumale | melanosporum | YNAU0126 | Yunnan, China | OR665398 | OR825717 | OR832383 | OR832415 |
Tuber pseudobrumale | melanosporum | YNAU0127 | Yunnan, China | OR665399 | OR661817 | OR832384 | OR832416 |
Tuber pseudobrumale | melanosporum | YNAU0128 | Yunnan, China | OR665400 | OR661818 | OR832385 | OR832417 |
Tuber pseudobrumale | melanosporum | YNAU0145 | Yunnan, China | OR665401 | OR661819 | OR832386 | OR832418 |
Tuber pseudobrumale | melanosporum | YNAU0148 | Yunnan, China | OR665402 | OR661820 | OR832387 | OR832419 |
Tuber pseudobrumale | melanosporum | YNAU0149 | Yunnan, China | OR665403 | OR661821 | OR832388 | OR832420 |
Tuber pseudobrumale | melanosporum | YNAU0150 | Yunnan, China | OR665404 | OR661822 | OR832389 | OR832421 |
Tuber pseudobrumale | melanosporum | YNAU0151 | Yunnan, China | OR665405 | OR661823 | OR832390 | OR832422 |
Tuber pseudobrumale | melanosporum | YNAU0152 | Yunnan, China | OR665406 | OR661824 | OR832391 | OR832423 |
Tuber pseudobrumale | melanosporum | YNAU0179 | Yunnan, China | OR665407 | OR661825 | OR832392 | OR832424 |
Tuber pseudobrumale | melanosporum | YNAU0180 | Yunnan, China | OR665408 | OR661826 | OR832393 | OR832425 |
Tuber pseudobrumale | melanosporum | YNAU0181 | Yunnan, China | OR665409 | OR661827 | OR832394 | OR832426 |
Tuber pseudobrumale | melanosporum | YNAU0221 | Yunnan, China | OR665410 | OR661828 | OR832395 | OR832427 |
Tuber pseudobrumale | melanosporum | YNAU0228 | Yunnan, China | OR665411 | OR661829 | OR832396 | OR832428 |
Tuber pseudobrumale | melanosporum | YNAU0229 | Yunnan, China | OR665412 | OR661830 | OR832397 | OR832429 |
Tuber pseudobrumale | melanosporum | YNAU0230 | Yunnan, China | OR665413 | OR661831 | OR832398 | OR832430 |
Tuber pseudobrumale | melanosporum | YNAU0233 | Yunnan, China | OR665414 | OR661832 | OR832399 | OR832431 |
Tuber pseudobrumale | melanosporum | YNAU0240 | Yunnan, China | OR665415 | OR661833 | OR832400 | OR832432 |
Tuber pseudobrumale | melanosporum | YNAU0275 | Yunnan, China | OR665416 | OR661834 | OR832401 | OR832433 |
Tuber pseudobrumale | melanosporum | YNAU0276 | Yunnan, China | OR665417 | OR661835 | OR832402 | OR832434 |
Tuber pseudobrumale | melanosporum | YNAU0277 | Yunnan, China | OR665418 | OR661836 | OR832403 | OR832435 |
Tuber pseudobrumale | melanosporum | YNAU0885 | Yunnan, China | OR665419 | OR661837 | OR832404 | OR832436 |
Tuber pseudofulgens | excavatum | BJTC FAN399, holotype | Yunnan, China | OM256757 | OM366196 | OM649601 | OM584259 |
Tuber pseudofulgens | excavatum | BJTCFAN388 | Sichuan, China | OM256755 | OM366194 | OM649599 | OM584257 |
Tuber pseudohimalayense | melanosporum | YNAU1608 | Yunnan, China | PP784763 | PP784589 | PP796851 | PP796861 |
Tuber pseudohimalayense | melanosporum | BJTC FAN122 | Sichan, China | MF627983 | OM366162 | OM649567 | OM584213 |
Tuber pseudohimalayense | melanosporum | YNAU1663 | Yunnan, China | PP784764 | PP784590 | PP796852 | PP796862 |
Tuber pseudohimalayense | melanosporum | YNAU1664 | Yunnan, China | PP784765 | PP784591 | PP796853 | PP796863 |
Tuber pseudohimalayense | melanosporum | YNAU1665 | Yunnan, China | PP784766 | PP784592 | PP796854 | PP796864 |
Tuber pseudohimalayense | melanosporum | YNAU1666 | Yunnan, China | PP784767 | PP784593 | PP796855 | PP796865 |
Tuber pseudohimalayense | melanosporum | YNAU1344 | Yunnan, China | PP784768 | PP784594 | PP796856 | PP796866 |
Tuber puberulum | puberulum | ZB1433 | Hungary | JF261382 | JF261346 | – | – |
Tuber puberulum | puberulum | ZB1983 | Hungary | JF261390 | JF261357 | – | – |
Tuber regimontanum | melanosporum | ITCV 909 | Mexico | EU375838 | FJ809823 | JX022600 | JQ954520 |
Tuber shidianense | puberulum | HKAS 88770, holotype | Yunnan, China | KT444595 | KY174960 | OR832405 | OR832437 |
Tuber shidianense | puberulum | HKAS 88771, paratype | Yunnan, China | KT444596 | KY174961 | OR832406 | OR832438 |
Tuber sinense | melanosporum | BJTC FAN108 | China | MF627968 | OM366160 | OM649565 | OM584211 |
Tuber sinense | melanosporum | BJTC FAN110 | China | MF627970 | OM366161 | OM649566 | OM584212 |
Tuber sinoaestivum | aestivum | BJTC FAN522 | Sichuan, China | OM256774 | OM366210 | OM649615 | OM584271 |
Tuber sinoaestivum | aestivum | BJTC FAN487 | Yunnan, China | OM256773 | OM366209 | OM649614 | OM584270 |
Tuber sinoexcavatum | excavatum | BJTC FAN130, holotype | Yunnan, China | JX458717 | OM366163 | OM649568 | OM584216 |
Tuber sinoexcavatum | excavatum | BJTC FAN166 | Yunnan, China | JX458718 | OM366165 | OM649571 | OM584221 |
Tuber sp. 1 | macrosporum | K201 | Japan | AB553344 | AB553512 | AB553532 | AB553552 |
Tuber sp. 5 KA-2010 | melanosporum | K229 | Japan | AB553381 | – | AB553536 | AB553556 |
Tuber sp. 5 KA-2010 | melanosporum | K403 | Japan | AB553382 | – | – | – |
Tuber sphaerosporum | puberulum | JT12487 | USA | FJ809853 | FJ809853 | JX022609 | – |
Tuber taiyuanense | rufum | BJTC FAN164 | Yunnan, China | OM311182 | OM366164 | OM649570 | OM584220 |
Tuber taiyuanense | rufum | BJTC FAN220 | Yunnan, China | MH115315 | OM366174 | OM649578 | OM584231 |
Tuber umbilicatum | rufum | BJTC FAN212 | Yunnan, China | OM311201 | OM366173 | OM649577 | OM584228 |
Tuber umbilicatum | rufum | BJTC FAN230 | Yunnan, China | OM311205 | OM366178 | OM649582 | OM584235 |
Tuber variabilisporum | melanosporum | BJTC FAN362, holotype | Sichuan, China | OM287845 | OM366190 | OM649595 | OM584253 |
Tuber variabilisporum | melanosporum | YNAU0146 | Yunnan, China | PP784759 | PP784585 | PP796847 | PP796857 |
Tuber variabilisporum | melanosporum | YNAU0468 | Yunnan, China | PP784760 | PP784586 | PP796848 | PP796858 |
Tuber variabilisporum | melanosporum | YNAU0925 | Yunnan, China | PP784761 | PP784587 | PP796849 | PP796859 |
Tuber variabilisporum | melanosporum | YNAU1670 | Yunnan, China | PP784762 | PP784588 | PP796850 | PP796860 |
Tuber wenchuanense | rufum | BJTC FAN833 | Shanxi, China | OM311256 | OM366222 | OM649629 | OM584280 |
Tuber yigongense | melanosporum | BJTC FAN729 | Tibet, China | MF663716 | – | OM649623 | OM584277 |
Tuber yigongense | melanosporum | BJTC FAN731, holotype | Tibet, China | MF663714 | OM366216 | – | – |
Tuber yunnanense | melanosporum | YNAU019, holotype | Yunnan, China | OK625306 | OR661811 | OR813081 | OR832407 |
Tuber yunnanense | melanosporum | YNAU020 | Yunnan, China | OK625307 | OR661812 | OR813082 | OR832408 |
Tuber yunnanense | melanosporum | YNAU0107 | Yunnan, China | OR665397 | OR661813 | OR813083 | OR832409 |
Tuber yunnanense | melanosporum | YNAU0491 | Sichuan, China | OR250186 | OR661814 | OR813084 | OR832410 |
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Wang, R.; Dong, G.; Li, Y.; Wang, R.; Yang, S.; Yuan, J.; Xie, X.; Shi, X.; Yu, J.; Pérez-Moreno, J.; et al. Three New Truffle Species (Tuber, Tuberaceae, Pezizales, and Ascomycota) from Yunnan, China, and Multigen Phylogenetic Arrangement within the Melanosporum Group. J. Fungi 2024, 10, 640. https://doi.org/10.3390/jof10090640
Wang R, Dong G, Li Y, Wang R, Yang S, Yuan J, Xie X, Shi X, Yu J, Pérez-Moreno J, et al. Three New Truffle Species (Tuber, Tuberaceae, Pezizales, and Ascomycota) from Yunnan, China, and Multigen Phylogenetic Arrangement within the Melanosporum Group. Journal of Fungi. 2024; 10(9):640. https://doi.org/10.3390/jof10090640
Chicago/Turabian StyleWang, Rui, Gangqiang Dong, Yupin Li, Ruixue Wang, Shimei Yang, Jing Yuan, Xuedan Xie, Xiaofei Shi, Juanbing Yu, Jesús Pérez-Moreno, and et al. 2024. "Three New Truffle Species (Tuber, Tuberaceae, Pezizales, and Ascomycota) from Yunnan, China, and Multigen Phylogenetic Arrangement within the Melanosporum Group" Journal of Fungi 10, no. 9: 640. https://doi.org/10.3390/jof10090640
APA StyleWang, R., Dong, G., Li, Y., Wang, R., Yang, S., Yuan, J., Xie, X., Shi, X., Yu, J., Pérez-Moreno, J., Yu, F., & Wan, S. (2024). Three New Truffle Species (Tuber, Tuberaceae, Pezizales, and Ascomycota) from Yunnan, China, and Multigen Phylogenetic Arrangement within the Melanosporum Group. Journal of Fungi, 10(9), 640. https://doi.org/10.3390/jof10090640