Next Article in Journal
Assembly of the Cardiac Pacemaking Complex: Electrogenic Principles of Sinoatrial Node Morphogenesis
Next Article in Special Issue
Persistent Ventricle Partitioning in the Adult Zebrafish Heart
Previous Article in Journal
Early Postnatal Cardiac Stress Does Not Influence Ventricular Cardiomyocyte Cell-Cycle Withdrawal
Previous Article in Special Issue
The Lymphatic System in Zebrafish Heart Development, Regeneration and Disease Modeling
Open AccessReview

Zebrafish as a New Tool in Heart Preservation Research

1
Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA 2114, USA
2
Shriners Hospitals for Children, Boston, MA 2114, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Mathilda Mommersteeg
J. Cardiovasc. Dev. Dis. 2021, 8(4), 39; https://doi.org/10.3390/jcdd8040039
Received: 11 March 2021 / Revised: 5 April 2021 / Accepted: 7 April 2021 / Published: 8 April 2021
(This article belongs to the Special Issue Zebrafish Heart Development, Regeneration, and Disease Modelling)
Heart transplantation became a reality at the end of the 1960s as a life-saving option for patients with end-stage heart failure. Static cold storage (SCS) at 4–6 °C has remained the standard for heart preservation for decades. However, SCS only allows for short-term storage that precludes optimal matching programs, requires emergency surgeries, and results in the unnecessary discard of organs. Among the alternatives seeking to extend ex vivo lifespan and mitigate the shortage of organs are sub-zero or machine perfusion modalities. Sub-zero approaches aim to prolong cold ischemia tolerance by deepening metabolic stasis, while machine perfusion aims to support metabolism through the continuous delivery of oxygen and nutrients. Each of these approaches hold promise; however, complex barriers must be overcome before their potential can be fully realized. We suggest that one barrier facing all experimental efforts to extend ex vivo lifespan are limited research tools. Mammalian models are usually the first choice due to translational aspects, yet experimentation can be restricted by expertise, time, and resources. Instead, there are instances when smaller vertebrate models, like the zebrafish, could fill critical experimental gaps in the field. Taken together, this review provides a summary of the current gold standard for heart preservation as well as new technologies in ex vivo lifespan extension. Furthermore, we describe how existing tools in zebrafish research, including isolated organ, cell specific and functional assays, as well as molecular tools, could complement and elevate heart preservation research. View Full-Text
Keywords: heart preservation; heart transplantation; regenerative medicine; zebrafish heart preservation; heart transplantation; regenerative medicine; zebrafish
Show Figures

Figure 1

MDPI and ACS Style

Da Silveira Cavalcante, L.; Tessier, S.N. Zebrafish as a New Tool in Heart Preservation Research. J. Cardiovasc. Dev. Dis. 2021, 8, 39. https://doi.org/10.3390/jcdd8040039

AMA Style

Da Silveira Cavalcante L, Tessier SN. Zebrafish as a New Tool in Heart Preservation Research. Journal of Cardiovascular Development and Disease. 2021; 8(4):39. https://doi.org/10.3390/jcdd8040039

Chicago/Turabian Style

Da Silveira Cavalcante, Luciana; Tessier, Shannon N. 2021. "Zebrafish as a New Tool in Heart Preservation Research" J. Cardiovasc. Dev. Dis. 8, no. 4: 39. https://doi.org/10.3390/jcdd8040039

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop