Marginal Zinc Deficiency Aggravated Intestinal Barrier Dysfunction and Inflammation through ETEC Virulence Factors in a Mouse Model of Diarrhea
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Treatment
2.2. Samples
2.3. Intestinal Morphology
2.4. Inflammatory Cytokines
2.5. Intestinal Permeability
2.6. Real-Time RT-PCR
2.7. Stool ETEC Shedding and Tissue Burden
2.8. ETEC Growth Curve
2.9. Zinc Content
2.10. Virulence Factor Analysis
2.11. Data Analysis
3. Results
3.1. Effect of Marginal Zinc Deficiency on Serum Zinc Concentration of Mice with ETEC Challenge
3.2. Marginal Zinc-Deficient Mice Have Greater Diarrhea Scores and Body Weight Losses after ETEC Challenge
3.3. Intestinal Morphology Was Altered in Marginal Zinc-Deficient Mice with ETEC Challenge
3.4. Marginal Zinc Deficiency Decreased Host Defense against ETEC Infection Induced Disruption of Intestinal Barrier and Permeability
3.5. Marginal Zinc Deficiency Aggravated Intestinal Inflammation in Mice with ETEC Challenge
3.6. Marginal Zinc Deficiency Aggravated Intestinal Injury Was Associated with NF-κB
3.7. Marginal Zinc Deficiency Altered Anion Transporters in Mice with ETEC Challenge
3.8. Marginal Zinc Deficiency Increased the ETEC Shedding in the Jejunum of Mice with ETEC Challenge
3.9. Marginal Zinc Deficiency Enhanced Virulence Factors in Mice with ETEC Infection
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, P.; Chen, Q.; Gan, L.; Du, X.; Li, Q.; Qiao, H.; Zhao, Y.; Huang, J.; Wang, J. Marginal Zinc Deficiency Aggravated Intestinal Barrier Dysfunction and Inflammation through ETEC Virulence Factors in a Mouse Model of Diarrhea. Vet. Sci. 2022, 9, 507. https://doi.org/10.3390/vetsci9090507
Wang P, Chen Q, Gan L, Du X, Li Q, Qiao H, Zhao Y, Huang J, Wang J. Marginal Zinc Deficiency Aggravated Intestinal Barrier Dysfunction and Inflammation through ETEC Virulence Factors in a Mouse Model of Diarrhea. Veterinary Sciences. 2022; 9(9):507. https://doi.org/10.3390/vetsci9090507
Chicago/Turabian StyleWang, Peng, Qianqian Chen, Liping Gan, Xinyu Du, Qiyue Li, Hanzhen Qiao, Yinli Zhao, Jin Huang, and Jinrong Wang. 2022. "Marginal Zinc Deficiency Aggravated Intestinal Barrier Dysfunction and Inflammation through ETEC Virulence Factors in a Mouse Model of Diarrhea" Veterinary Sciences 9, no. 9: 507. https://doi.org/10.3390/vetsci9090507