Molecular Characterization of ASFV and Differential Diagnosis of Erysipelothrix in ASFV-Infected Pigs in Pig Production Regions in Cameroon
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethical Approval
2.2. Study Area
2.3. Study Design
2.4. Sample Collection
2.5. DNA Extraction and ASFV Detection
2.6. ASFV Molecular Characterization, Nucleotide Sequencing and Analysis
2.7. DNA Extraction and Erysipelothrix Detection
3. Results
3.1. Diagnosis of ASFV by PCR
3.2. B646L (P72) Gene Phylogeny
3.3. E183L (P54) Gene Phylogeny
3.4. CP204L (P30) Gene Phylogeny
3.5. Intergenic Region of I73R and I329L
3.6. Analysis of the B602L Gene
3.7. Analysis of the EP402R Gene
3.8. Differential Diagnosis of Erysipelothrix
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Collection Site | Region | Division | Farm Location or Slab | Number of Samples Collected | Total |
---|---|---|---|---|---|
Slaughter slabs (blood and tissue samples) | Southwest | Fako | Mile 16 Buea | 24 | 100 |
Liottoral | Moungo | Grand Hanger market (Bonaberi) | 22 | ||
Outbreak collection | Southwest | Fako | `Buea Misselleleh | 30 24 | |
From pig farms (Blood only) | Littoral | Wouri | Bonaberi | 15 | 37 |
Moungo | Souza | 22 | |||
Southwest | Fako | Buea | 69 | 143 | |
Limbe | 26 | ||||
Misselleleh Area | 48 | ||||
West | Mifi | Baffousam | 8 | 20 | |
Koung Khi | Baham | 4 | |||
Bandjoun | 8 | ||||
Northwest | Mezam | Bafut | 22 | 77 | |
Santa | 55 |
Number of CVR Repeats | CVR Tandem Repeat Sequence (TRS) Signature | Accession Numbers of Sequences Involved |
---|---|---|
19 | ABNAAAACBNABTDBNAFA | ON191096, ON191099, ON191100, ON191102, ON191103, ON191104, ON191106, ON191107, ON191110, ON191111, ON191112, ON191113, ON191115, ON191119, ON191121, ON191123, ON191124, ON191127, ON191128, ON191133, ON191134, ON191139, ON191146, ON191155, ON191157, ON191158, ON191160, ON191161, |
6 | AB--------------------NAFA | ON191097, ON191098, ON191101, ON191105, ON191108, ON191109, ON191114, ON191116, ON191117, ON191118, ON191120, ON191122, ON191125, ON191126, ON191129, ON191130, ON191131, ON191132, ON191135, ON191136, ON191137, ON191138, ON191140, ON191141, ON191142, ON191143, ON191144, ON191145, ON191147, ON191148, ON191149, ON191150, ON191151, ON191152, ON191153, ON191154, ON191156, ON191159, ON191162, ON191163, ON191164, ON191165, ON191166, ON191167, ON191168, ON191169, ON191170, ON191171, ON191172, ON191173, ON191174, ON191175, ON191176, ON191177, ON191178, ON191179, ON191180, ON191181, ON191182, ON191183, ON191184, ON191185, ON191186, ON191187, ON191188, ON191189, ON191190, ON191191, ON191192, ON191193, ON191194, ON191195, ON191196, ON191197, ON191198, ON191199, ON191200, ON191201, ON191202. |
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Ebwanga, E.J.; Ghogomu, S.M.; Paeshuyse, J. Molecular Characterization of ASFV and Differential Diagnosis of Erysipelothrix in ASFV-Infected Pigs in Pig Production Regions in Cameroon. Vet. Sci. 2022, 9, 440. https://doi.org/10.3390/vetsci9080440
Ebwanga EJ, Ghogomu SM, Paeshuyse J. Molecular Characterization of ASFV and Differential Diagnosis of Erysipelothrix in ASFV-Infected Pigs in Pig Production Regions in Cameroon. Veterinary Sciences. 2022; 9(8):440. https://doi.org/10.3390/vetsci9080440
Chicago/Turabian StyleEbwanga, Ebanja Joseph, Stephen Mbigha Ghogomu, and Jan Paeshuyse. 2022. "Molecular Characterization of ASFV and Differential Diagnosis of Erysipelothrix in ASFV-Infected Pigs in Pig Production Regions in Cameroon" Veterinary Sciences 9, no. 8: 440. https://doi.org/10.3390/vetsci9080440