Chitosan/Calcium-Coated Ginsenoside Rb1 Phosphate Flower-like Microparticles as an Adjuvant to Enhance Immune Responses
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Preparation of CS/CaP Flower-like Microparticles and GRb1/IL-4@CS/CaP Microparticles
2.3. Measurement of Particle Size of GRb1/IL-4@CS/CaP Microparticles
2.4. Animal Immunization
2.5. Viability of DCs
2.6. Investigation of DC Activation and Maturation, Cytokine Secretion in Bone Marrow Cells
2.7. ELISA Analysis of Antibodies
2.8. Determination of Cytokine Levels
2.9. Statistical Analysis
3. Results
3.1. Characterization of CS/CaP and GRb1/IL-4@CS/CaP Microparticles
3.2. Encapsulation Efficiency of CS/CaP and GRb1/IL-4@CS/CaP Microparticles
3.3. Stability of CS/CaP and GRb1/IL-4@CS/CaP
3.4. Viability of DCs
3.5. Surface Molecular Expression Levels and Cytokine Secretion in DCs
3.6. IBDV-Specific Antibody Response in Vaccinated Chicken
3.7. Cytokine Levels in Chicken Serum
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Song, X.; Li, H.; Zhang, L.; Zhang, X.; Zhao, L.; Zhang, G.; Cao, S.; Liu, Y. Chitosan/Calcium-Coated Ginsenoside Rb1 Phosphate Flower-like Microparticles as an Adjuvant to Enhance Immune Responses. Vet. Sci. 2022, 9, 355. https://doi.org/10.3390/vetsci9070355
Song X, Li H, Zhang L, Zhang X, Zhao L, Zhang G, Cao S, Liu Y. Chitosan/Calcium-Coated Ginsenoside Rb1 Phosphate Flower-like Microparticles as an Adjuvant to Enhance Immune Responses. Veterinary Sciences. 2022; 9(7):355. https://doi.org/10.3390/vetsci9070355
Chicago/Turabian StyleSong, Xinghui, Huijuan Li, Liheng Zhang, Xiaozhan Zhang, Li Zhao, Gaiping Zhang, Shengbo Cao, and Yunchao Liu. 2022. "Chitosan/Calcium-Coated Ginsenoside Rb1 Phosphate Flower-like Microparticles as an Adjuvant to Enhance Immune Responses" Veterinary Sciences 9, no. 7: 355. https://doi.org/10.3390/vetsci9070355