Immunohistochemical Expression of Platelet-Derived Growth Factor Receptor β (PDGFR-β) in Canine Cutaneous Peripheral Nerve Sheath Tumors: A Preliminary Study
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Samples and Histopathology
2.2. Immunohistochemistry (IHC)
2.3. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Case Nr. | Breed | Age (Years) | Gender | Type of Tumor | Location of Tumor | Dimensions of Tumor (cm) | Ki-67 PI (%) | % PDGFR-β Expression |
---|---|---|---|---|---|---|---|---|
1 | M. Schnauzer | 13 | M | B | T | 2.5 × 2.5 | 60.8 | ≤25% |
2 | Mixed breed | 12 | F | B | L | 4 × 3.5 | 15.8 | >25% |
3 | Poodle | 9 | M | B | NR | 3 × 2.5 | 36.3 | >25% |
4 | X Rottweiler | 8 | M | B | NR | NR | 4.3 | ≤25% |
5 | Mixed breed | 8 | M | B | L | 4 × 2.8 | 1.8 | ≤25% |
6 | Mixed breed | 10 | F | B | L | 2 × 1 | 3.8 | ≤25% |
7 | G. Shepherd | 14 | F | B | T | 4 × 2 | 14.1 | ≤25% |
8 | Poodle | 13 | M | B | L | 1.2 × 1.2 | 1.2 | ≤25% |
9 | Boxer | 9 | M | B | T | 2 × 1 | 2.8 | >25% |
10 | Port. Pointer | 9 | M | M | T | 8 × 8 | 38.9 | >25% |
11 | St. Bernard | 10 | F | M | L | 10 × 10 | 62.6 | ≤25% |
12 | Siberian husky | 13 | F | M | L | 4.5 × 4 | 14.1 | >25% |
13 | Labr. Retriever | 2 | M | M | T | 3 × 3 | 23.5 | >25% |
14 | Boxer | 7 | M | M | L | 4.5 × 3.5 | 26.2 | >25% |
15 | Boxer | 7 | F | M | T | 6.5 × 6 | 21.4 | >25% |
16 | Mixed breed | 12 | M | M | L | 3 × 2 | 54.0 | ≤25% |
17 | Mixed breed | 12 | F | M | T | 10.5 × 8 | 6.4 | >25% |
18 | Dalmatian | 10 | F | M | L | 4 × 2.5 | 10.2 | >25% |
19 | Boxer | 8 | F | M | L | 4 × 2 | 2.8 | >25% |
Variable | Nr. of Tumors | BPNST (Number; %) | MPNST (Number; %) | p Value |
---|---|---|---|---|
Gender | ||||
Female | 9 | 3 (33.3) | 6 (66.7) | 0.24 |
Male | 10 | 6 (60.0) | 4 (40.0) | |
Breed * | ||||
Small | 3 | 3 (100.0) | 0 (0) | 0.05 |
Medium, Large, Giant | 11 | 3 (27.3) | 8 (72.7) | |
Tumor Location * | ||||
Trunk | 7 | 3 (42.9) | 4 (57.1) | 0.64 |
Limbs | 10 | 4 (40.0) | 6 (60.0) | |
Tumor size * | ||||
<3 cm | 4 | 4 (100.0) | 0 (0) | 0.02 |
≥3 cm | 14 | 4 (28.6) | 10 (71.4) |
Variable | Nr. of Tumors | Mean Ki-67 | Standard Deviation (SD) | p Value (ANOVA) |
---|---|---|---|---|
Tumor Type | ||||
Benign | 9 | 15.66 | 20.32 | 0.28 |
Malignant | 10 | 26.01 | 20.09 | |
PDGFR-β expression | ||||
≤25% | 8 | 25.33 | 28.38 | 0.45 |
>25% | 11 | 18.04 | 12.48 |
Variable | Nr. of Tumors | Tumors with ≤25% PDGFR-β Expression (Number; %) | Tumors with >25% PDGFR-β Expression (Number; %) | p Value |
---|---|---|---|---|
Tumor type | ||||
Benign | 9 | 6 (66.7) | 3 (33.3) | 0.05 |
Malignant | 10 | 2 (20.0) | 8 (80.0) | |
Tumor Location * | ||||
Trunk | 7 | 2 (28.6) | 5 (71.4) | 0.35 |
Limbs | 10 | 5 (50.0) | 5 (50.0) | |
Tumor size * | ||||
<3 cm | 4 | 3 (75.0) | 1 (25.0) | 0.13 |
≥3 cm | 14 | 4 (28.6) | 10 (71.4) | |
Ki-67 Index | ||||
<20% | 11 | 5 (45.5) | 6 (54.5) | 0.55 |
≥20% | 8 | 3 (37.5) | 5 (62.5) |
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Aluai-Cunha, C.; Matos, A.; Amorim, I.; Carvalho, F.; Rêma, A.; Santos, A. Immunohistochemical Expression of Platelet-Derived Growth Factor Receptor β (PDGFR-β) in Canine Cutaneous Peripheral Nerve Sheath Tumors: A Preliminary Study. Vet. Sci. 2022, 9, 345. https://doi.org/10.3390/vetsci9070345
Aluai-Cunha C, Matos A, Amorim I, Carvalho F, Rêma A, Santos A. Immunohistochemical Expression of Platelet-Derived Growth Factor Receptor β (PDGFR-β) in Canine Cutaneous Peripheral Nerve Sheath Tumors: A Preliminary Study. Veterinary Sciences. 2022; 9(7):345. https://doi.org/10.3390/vetsci9070345
Chicago/Turabian StyleAluai-Cunha, Catarina, Augusto Matos, Irina Amorim, Fátima Carvalho, Alexandra Rêma, and Andreia Santos. 2022. "Immunohistochemical Expression of Platelet-Derived Growth Factor Receptor β (PDGFR-β) in Canine Cutaneous Peripheral Nerve Sheath Tumors: A Preliminary Study" Veterinary Sciences 9, no. 7: 345. https://doi.org/10.3390/vetsci9070345