Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk
Abstract
:1. Introduction
2. Materials and Methods
2.1. Schematic Overview of the Experimental Program
2.2. Serum and Milk Controls
2.3. Serum and Milk Field Samples
2.4. Milk Treatment
2.5. Serological Tests
2.6. Fluorescence Polarization Assay
2.7. Statistical Analysis
3. Results
3.1. Fluorescence Polarization Assay on Treated and Untreated Control Milk Samples
3.2. The FPA in Control Milk Samples
3.3. The FPA on Milk Samples
3.4. Comparison of FPA and PT3 Test Performance on Milk Samples, Using the Serum FPA as the Reference Test
3.5. Comparison of FPA in Serum Performance with FPA in Milk and RBT
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Milk FPA versus RBT 3 | |
---|---|
Difference between areas | 0.0135 |
SEM | 0.0766 |
95% Confidence interval | −0.137 to 0.164 |
z Statistic | 0.176 |
Significance level | p = 0.8601 |
TEST | SE | 95% CI | SP | 95% CI | AUC |
---|---|---|---|---|---|
RBT 3 | 99.26 | 95.9–100.0 | 83.33 | 51.6–97.9 | 0.913 |
SERUM FPA | 88.89 | 73.9–96.9 | 100.0 | 93.6–100.0 | 99.26 |
MILK FPA | 83.33 | 62.6–95.3 | 75.93 | 62.4–86.5 | 0.878 |
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Sotolongo-Rodríguez, D.; Gomez-Flores, R.; Navarro-Soto, M.C.; Arellano-Reynoso, B.; Tamez-Guerra, P.; Ramírez-Pfeiffer, C. Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk. Vet. Sci. 2022, 9, 303. https://doi.org/10.3390/vetsci9060303
Sotolongo-Rodríguez D, Gomez-Flores R, Navarro-Soto MC, Arellano-Reynoso B, Tamez-Guerra P, Ramírez-Pfeiffer C. Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk. Veterinary Sciences. 2022; 9(6):303. https://doi.org/10.3390/vetsci9060303
Chicago/Turabian StyleSotolongo-Rodríguez, Dianelys, Ricardo Gomez-Flores, Magda Celina Navarro-Soto, Beatriz Arellano-Reynoso, Patricia Tamez-Guerra, and Carlos Ramírez-Pfeiffer. 2022. "Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk" Veterinary Sciences 9, no. 6: 303. https://doi.org/10.3390/vetsci9060303