E-cadherin Expression in Canine Gastric Carcinomas: Association with Clinicopathological Parameters
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Selection
2.2. Histological Classification
2.3. Immunohistochemistry (IHC)
2.4. Immunohisochemistry Evaluation of E-cadherin
2.5. Statistical Analysis
3. Results
3.1. Clinicopathological Data
3.2. Immunohistochemistry
3.2.1. Normal Gastric Mucosa
3.2.2. Non-Neoplastic Gastric Mucosa Adjacent to Carcinomas
3.2.3. Gastric Carcinomas
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Case No. | Breed | Sex/ Age (Years) | Weight, kg | Tumor Location | Histological Classification | Metastases | E-cad Immunoexpression (Score) | ||||
---|---|---|---|---|---|---|---|---|---|---|---|
WHO Classification | Lauren | Primary Tumors | Emboli | Metastases | |||||||
1 | Crossbreed | F/13 | ≤10 | Body | Tubular | Intestinal | - | N (3) | - | - | |
2 | Labrador Retriever | F/14 | 26–45 | Body | Tubular | Intestinal | NA | Ab (2) | - | - | |
3 | Collie | M/11 | 26–45 | Body and antrum | Tubular | Intestinal | Lymph node, Pancreas, Intestine * | Ab (2) | N (3) | Ab (2) | |
4 | Miniature Poodle | F/14 | ≤10 | Antrum | Tubular | Intestinal | - | N (3) | - | - | |
5 | Basset Hound | F/12 | 26–45 | Antrum | Tubular | Intestinal | Lymph node * | Ab (2) | Ab (2) | Ab (2) | |
6 | Siberian Husky | F/12 | 26–45 | Antrum | Tubular | Intestinal | NA | Ab (2) | Ab (2) | - | |
7 | Siberian Husky | M/13 | 26–45 | Antrum | Tubular | Intestinal | - | Ab (2) | - | - | |
8 | Crossbreed (X poodle) | F/9 | 11–25 | Antrum | Papillary | Intestinal | - | N (3) | - | - | |
9 | Crossbreed | M/10 | NR | Body | Mucinous | Diffuse | NA | Ab (2) | N (3) | - | |
10 | Chow-Chow | M/6 | 26–45 | Body | Mucinous | Diffuse | - | Ab (2) | Ab (2) | ||
11 | English Bulldog | M/6 | 11–25 | Body | Signet ring cell | Diffuse | - | Ab (2) | - | - | |
12 | Sharpei | M/5 | 11-25 | Body | Signet ring cell | Diffuse | - | Ab (2) | NA | - | |
13 | Golden Retriever | M/14 | 26–45 | Body | Signet ring cell | Diffuse | Lung *, Esophagus, Liver, Adrenal gland * | Ab (2) | Ab (0) | Ab (2) | |
14 | Pointer | M/11 | 26–45 | Body | Signet ring cell | Diffuse | Lymph node * | Ab (2) | Ab (2) | Ab (2) | |
15 | Crossbreed | F/7 | ≤10 | Body and antrum | Signet ring cell | Diffuse | - | Ab (2) | - | - | |
16 | Cocker Spaniel | M/13 | 11–25 | Antrum | Signet ring cell | Diffuse | - | Ab (2) | - | - | |
17 | Chow-Chow | M/10 | 26–45 | Antrum | Signet ring cell | Diffuse | - | Ab (0) | - | - | |
18 | Golden Retriever | M/10 | 26–45 | Antrum | Signet ring cell | Diffuse | - | Ab (2) | - | - | |
19 | Boxer | M/7 | 26–45 | Antrum | Signet ring cell | Diffuse | - | N (3) | - | - | |
20 | West Highland White Terrier | F/13 | ≤10 | Antrum | Signet ring cell | Diffuse | - | Ab (2) | Ab (2) | - | |
21 | Alaska Malamute | M/6 | 26–45 | NA | Signet ring cell | Diffuse | - | Ab (2) | - | - | |
22 | Crossbreed | F/8 | 11–25 | Body | Poorly cohesive | Diffuse | Intestine *, Peritoneum, Liver | Ab (2) | N (3) | N (3) | |
23 | Crossbreed (X German Shepherd) | F/13 | 26–45 | Body | Poorly cohesive | Diffuse | Liver | Ab (0) | - | - | |
24 | Akita | M/9 | 26–45 | Body | Poorly cohesive | Diffuse | NA | Ab (0) | Ab (2) | - | |
25 | German Shepherd | M/12 | 26–45 | Body and antrum | Poorly cohesive | Diffuse | Esophagus *, Lymph node | Ab (2) | Ab (2) | Ab (2) | |
26 | Shih Tzu | F/10 | ≤10 | Antrum | Poorly cohesive | Diffuse | - | Ab (2) | NA | - | |
27 | Chow-Chow | M/9 | 26–45 | Antrum | Poorly cohesive | Diffuse | - | Ab (2) | - | - | |
28 | Crossbreed | F/7 | NR | Antrum | Poorly cohesive | Diffuse | Intestine * | Ab (2) | - | N (3) | |
Intestinal component | Diffuse component | ||||||||||
29 | Belgian Shepherd | F/11 | 11–25 | Body | Mixed | Indeterminate | Lymph node * | Ab (2) | Ab (2) | Ab (2) | Ab (2) |
30 | Collie | M/11 | 26–45 | Body | Mixed | Indeterminate | - | Ab (2) | N (3) | N (3) | - |
31 | Chow-Chow | F/11 | 26–45 | Antrum | Mixed | Indeterminate | - | Ab (2) | Ab (2) | N (3) | - |
32 | Labrador Retriever | M/8 | 26–45 | Antrum | Mixed | Indeterminate | NA | Ab (2) | N (3) | Ab (2) | - |
33 | Standard Poodle | M/8 | 11–25 | Antrum | Mixed | Indeterminate | - | Ab (2) | Ab (2) | N (3) | - |
No. of Cases | Histological Diagnosis | p-Value | Tumor Differentiation | p-Value | ||||
---|---|---|---|---|---|---|---|---|
Intestinal | Diffuse | Indeterminate | Well- Differentiated | Poorly/ Undifferentiated | ||||
Sex | ||||||||
Male | 19 | 2 | 14 | 3 | 0.0929 | 2 | 17 | 0.0322 |
Female | 14 | 6 | 6 | 2 | 6 | 8 | ||
Age, years | ||||||||
<10 | 13 | 1 | 10 | 2 | 0.1858 | 1 | 12 | 0.0737 |
≥10 | 20 | 7 | 10 | 3 | 7 | 13 | ||
Weight, kg | ||||||||
≤10 | 5 | 2 | 3 | 0 | 0.6924 | 2 | 3 | 0.6023 |
11–25 | 7 | 1 | 4 | 2 | 1 | 6 | ||
26–45 | 19 | 5 | 11 | 3 | 5 | 14 |
Clinicopathological Parameters | No. of Cases | E-cad Immunoexpression | p-Value | |
---|---|---|---|---|
Normal (n = 4) | Abnormal (n = 29) | |||
n (%) | n (%) | |||
Sex | ||||
Male | 19 | 1 (5.3%) | 18 (94.7%) | 0.1597 |
Female | 14 | 3 (21.4%) | 11 (78.6%) | |
Age, years | ||||
<10 | 13 | 2 (15,4%) | 11 (84.6%) | 0.6433 |
≥10 | 20 | 2 (10.0%) | 18 (90.0%) | |
Weight, kg a | ||||
≤10 | 5 | 2 (40%) | 3 (60%) | 0.1185 |
11–25 | 7 | 1 (14.3%) | 6 (85.7%) | |
26–45 | 19 | 1 (5.3%) | 18 (94.7%) | |
Tumor location b | ||||
Antrum | 16 | 3 (18.8%) | 13 (81.3%) | 0.5287 |
Body | 13 | 1 (7.7%) | 12 (92.3%) | |
Body and antrum | 3 | 0 (0%) | 3 (100%) | |
Histological diagnosis | ||||
WHO classification | ||||
Tubular | 7 | 2 (28.6%) | 5 (71.4%) | 0.0503 |
Papillary | 1 | 1 (100%) | 0 (0%) | |
Mucinous | 2 | 0 (0%) | 2 (100%) | |
Signet ring cell | 11 | 1 (9.1%) | 10 (90.9%) | |
Poorly cohesive | 7 | 0 (0%) | 7 (100%) | |
Mixed | 5 | 0 (0%) | 5 (100%) | |
Lauren | ||||
Intestinal | 8 | 3 (37.5%) | 5 (62.5%) | 0.0392 |
Diffuse | 20 | 1 (5.0%) | 19 (95.0%) | |
Indeterminate | 5 | 0 (0%) | 5 (100%) | |
Tumor differentiation | ||||
Well-differentiated | 8 | 3 (37.5%) | 5 (62.5%) | 0.0115 |
Poorly/undifferentiated | 25 | 1 (4.0%) | 24 (96.0%) | |
Depth of tumor invasion c | ||||
Mucosa | 1 | 1 (100%) | 0 (0%) | |
Muscular | 10 | 2 (20.0%) | 8 (80.0%) | 0.0117 |
Serosa | 12 | 0 (0%) | 12 (100%) | |
Neoplastic emboli | ||||
Present | 18 | 0 (0%) | 18 (100%) | 0.0194 |
Absent | 15 | 4 (26.7%) | 11 (73.3%) | |
Metastatic lesions d | ||||
Present | 9 | 0 (0%) | 9 (100%) | 0.1371 |
Absent | 19 | 4 (21.1%) | 15 (78.9%) |
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Flores, A.R.; Rêma, A.; Mesquita, J.R.; Taulescu, M.; Seixas, F.; Gärtner, F.; Amorim, I. E-cadherin Expression in Canine Gastric Carcinomas: Association with Clinicopathological Parameters. Vet. Sci. 2022, 9, 172. https://doi.org/10.3390/vetsci9040172
Flores AR, Rêma A, Mesquita JR, Taulescu M, Seixas F, Gärtner F, Amorim I. E-cadherin Expression in Canine Gastric Carcinomas: Association with Clinicopathological Parameters. Veterinary Sciences. 2022; 9(4):172. https://doi.org/10.3390/vetsci9040172
Chicago/Turabian StyleFlores, Ana R., Alexandra Rêma, João R. Mesquita, Marian Taulescu, Fernanda Seixas, Fátima Gärtner, and Irina Amorim. 2022. "E-cadherin Expression in Canine Gastric Carcinomas: Association with Clinicopathological Parameters" Veterinary Sciences 9, no. 4: 172. https://doi.org/10.3390/vetsci9040172