16S rRNA Gene Sequencing Revealed Changes in Gut Microbiota Composition during Pregnancy and Lactation in Mice Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals, Experimental Design, and Sample Collection
2.2. Identification of Mouse Estrus Cycle Stage
2.3. Sex Hormones Measurements
2.4. Analysis of Cecum Short-Chain Fatty Acids (SCFAs)
2.5. DNA Extraction, PCR Amplification, and 16S rRNA Gene Sequencing
2.6. 16S rRNA Gene Sequencing Data Analysis
2.7. Statistical Analysis
3. Results
3.1. Weight and SERUM Sex Hormones Changes during Pregnancy and Lactation
3.2. Microbial Community Profiles during Pregnancy and Lactation in Mice
3.3. Comparison of the Gut Microbiota during Pregnancy and Lactation
3.4. Fecal SCFAs Concentrations during Pregnancy and Lactation in Mice
3.5. Correlation Analysis of Caecal Microbiota Composition and SCFAs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Guo, M.; Cao, X.; Zhang, K.; Pan, M.; Wu, Y.; Langda, S.; Yang, Y.; Chen, Y.; Gui, B.; Ma, B. 16S rRNA Gene Sequencing Revealed Changes in Gut Microbiota Composition during Pregnancy and Lactation in Mice Model. Vet. Sci. 2022, 9, 169. https://doi.org/10.3390/vetsci9040169
Guo M, Cao X, Zhang K, Pan M, Wu Y, Langda S, Yang Y, Chen Y, Gui B, Ma B. 16S rRNA Gene Sequencing Revealed Changes in Gut Microbiota Composition during Pregnancy and Lactation in Mice Model. Veterinary Sciences. 2022; 9(4):169. https://doi.org/10.3390/vetsci9040169
Chicago/Turabian StyleGuo, Mengmeng, Xi Cao, Ke Zhang, Menghao Pan, Yujiang Wu, Suo Langda, Yuxin Yang, Yulin Chen, Ba Gui, and Baohua Ma. 2022. "16S rRNA Gene Sequencing Revealed Changes in Gut Microbiota Composition during Pregnancy and Lactation in Mice Model" Veterinary Sciences 9, no. 4: 169. https://doi.org/10.3390/vetsci9040169