Humoral Responses and Ex Vivo IFN-γ Production after Canine Whole Blood Stimulation with Leishmania infantum Antigen or KMP11 Recombinant Protein
Abstract
:1. Introduction
2. Materials and Methods
2.1. Dogs
2.2. Antigen Preparation
2.3. Serological Diagnosis by ELISA
2.3.1. Leishmania infantum ELISA
2.3.2. KMP11 ELISA
2.4. Whole Blood Assay
2.5. Sandwich ELISA for the Determination of IFN-γ
2.6. Statistical Analysis
3. Results
3.1. Antibody Response
3.2. Ex Vivo IFN-γ-Release Whole Blood Assay
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Total Dogs (n = 108) | Positive Antibody Response | |
---|---|---|
L. infantum N (%) | rKMP11 N (%) | |
Group 1 (n = 26) | 0 (0) | 0 (0) |
Group 2 (n = 46) | ||
Stage I (n = 5) Stage IIa (n = 22) Stage IIb (n = 7) Stage III (n = 8) Stage IV (n = 4) | 1 (3.1) 22 (47.8) 7 (15.2) 8 (17.3) 4 (8.6) | 0 (0) 12 (26.0) 6 (13.0) 5 (10.8) 3 (6.5) |
Group 3 (n = 22) | 4 (18.1) | 0 (0) |
Group 4 (n = 14) | 0 (0) | 0 (0) |
Total | 46 (42.5) | 26 (24) |
Total Dogs (n = 69) | IFNγ Response | |
---|---|---|
LSA N (%) | rKMP11 N (%) | |
Group 1 (n = 25) | 0 (0) | 0 (0) |
Group 2 (n = 15) | ||
Stage I (n = 6) Stage IIa (n = 6) Stage IIb (n = 2) Stage III (n = 1) | 2 (13.3) 2 (13.3) 1 (6.6) 0 (0) | 2 (13.3) 0 (0) 0 (0) 0 (0) |
Group 3 (n = 16) | 12 (75) | 0 (0) |
Group 4 (n = 13) | 7 (53.8) | 0 (0) |
Total | 24 (34.7) | 2 (2.8) |
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Martínez-Orellana, P.; González, N.; Baldassarre, A.; Álvarez-Fernández, A.; Ordeix, L.; Paradies, P.; Soto, M.; Solano-Gallego, L. Humoral Responses and Ex Vivo IFN-γ Production after Canine Whole Blood Stimulation with Leishmania infantum Antigen or KMP11 Recombinant Protein. Vet. Sci. 2022, 9, 116. https://doi.org/10.3390/vetsci9030116
Martínez-Orellana P, González N, Baldassarre A, Álvarez-Fernández A, Ordeix L, Paradies P, Soto M, Solano-Gallego L. Humoral Responses and Ex Vivo IFN-γ Production after Canine Whole Blood Stimulation with Leishmania infantum Antigen or KMP11 Recombinant Protein. Veterinary Sciences. 2022; 9(3):116. https://doi.org/10.3390/vetsci9030116
Chicago/Turabian StyleMartínez-Orellana, Pamela, Noemí González, Antonella Baldassarre, Alejandra Álvarez-Fernández, Laura Ordeix, Paola Paradies, Manuel Soto, and Laia Solano-Gallego. 2022. "Humoral Responses and Ex Vivo IFN-γ Production after Canine Whole Blood Stimulation with Leishmania infantum Antigen or KMP11 Recombinant Protein" Veterinary Sciences 9, no. 3: 116. https://doi.org/10.3390/vetsci9030116