LncRNA Expression Profiles in Canine Mammary Tumors Identify lnc34977 as a Promoter of Proliferation, Migration and Invasion of Canine Mammary Tumor Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Treatment
2.2. RNA Extraction and High-Throughput Sequencing
2.3. Bioinformatics Analysis of Differentially Expressed lncRNAs
2.4. Quantitative Real-Time Polymerase Chain Reaction (RT–qPCR) Analysis
2.5. Cells and Culture
2.6. Cell Counting kit-8 Assay
2.7. Colony Formation Assay
2.8. Wound Healing Assay
2.9. Cell Invasion Assays
2.10. Mammary Tumor-Bearing Nude Mice Model
2.11. Statistical Analysis
3. Results
3.1. Sample Collection and Identification
3.2. lncRNA and mRNA Profiles
3.3. Differentially Expressed Gene Analysis from a Public Database
3.4. GO and KEGG Pathway Analysis of Common Differentially Expressed Genes
3.5. lncRNA–miRNA–mRNA Network
3.6. Expression of Differential lncRNAs Verified by RT-qPCR
3.7. Overexpression of lnc40589 and Knockdown of lnc34977 Inhibited the Proliferation of CMT Cells
3.8. Overexpression of lnc40589 and Knockdown of lnc34977 Inhibited the Migration of CMT Cells
3.9. Overexpression of lnc40589 and Knockdown of lnc34977 Inhibited Invasion of CMT Cells
3.10. Knockdown of lnc34977 in a Mammary Tumor-Bearing Nude Mouse Model
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lu, B.; Wu, J.; Chen, H.; Li, S.; Jia, K. LncRNA Expression Profiles in Canine Mammary Tumors Identify lnc34977 as a Promoter of Proliferation, Migration and Invasion of Canine Mammary Tumor Cells. Vet. Sci. 2022, 9, 82. https://doi.org/10.3390/vetsci9020082
Lu B, Wu J, Chen H, Li S, Jia K. LncRNA Expression Profiles in Canine Mammary Tumors Identify lnc34977 as a Promoter of Proliferation, Migration and Invasion of Canine Mammary Tumor Cells. Veterinary Sciences. 2022; 9(2):82. https://doi.org/10.3390/vetsci9020082
Chicago/Turabian StyleLu, Baochun, Juye Wu, Hebi Chen, Shoujun Li, and Kun Jia. 2022. "LncRNA Expression Profiles in Canine Mammary Tumors Identify lnc34977 as a Promoter of Proliferation, Migration and Invasion of Canine Mammary Tumor Cells" Veterinary Sciences 9, no. 2: 82. https://doi.org/10.3390/vetsci9020082