Detection and Characterization of a Novel Picornavirus in European Badger (Meles meles)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sampling
2.2. Molecular Investigation for PVs
2.3. Oxford Nanopore Technologies (ONT) Sequencing
2.4. Virus Isolation
2.5. RT-PCR for Sakobuviruses
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primers | Target Genes | Assay | Sequence (5′ to 3′) | Position | References |
---|---|---|---|---|---|
3D-for | 3DRdRp | Screening RT-PCR | GTGGGCTGCAAYCCNGA | 7438–7454 | [3] |
3D-rev | 3DRdRp | Screening RT-PCR | TTNAGNGCATCAAACCARA | 7544–7563 | [3] |
FR26RV-N | -- | cDNA synthesis | GCCGGAGCTCTGCAGATATC-N6 | -- | [17] |
FR40RV-T | Poly-(A) tail | cDNA synthesis | GCCGGAGCTCTGCAGATATC-T20 | -- | [18] |
FR20RV | --- | SISPA | GCCGGAGCTCTGCAGATATC | -- | [17] |
SaKoV-for | 3DRdRp | pan-SaKoV RT-PCR | GGTAGCGCGGTCGGTTGCGACCC | 6862–6884 | This study |
SaKoV-rev | 3DRdRp | pan-SaKoV RT-PCR | CCCAGGACTGGTAGTTGTTAG | 7529–7550 | This study |
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Palombieri, A.; Fruci, P.; Sarchese, V.; Robetto, S.; Orusa, R.; Arbuatti, A.; Martella, V.; Di Martino, B.; Di Profio, F. Detection and Characterization of a Novel Picornavirus in European Badger (Meles meles). Vet. Sci. 2022, 9, 645. https://doi.org/10.3390/vetsci9110645
Palombieri A, Fruci P, Sarchese V, Robetto S, Orusa R, Arbuatti A, Martella V, Di Martino B, Di Profio F. Detection and Characterization of a Novel Picornavirus in European Badger (Meles meles). Veterinary Sciences. 2022; 9(11):645. https://doi.org/10.3390/vetsci9110645
Chicago/Turabian StylePalombieri, Andrea, Paola Fruci, Vittorio Sarchese, Serena Robetto, Riccardo Orusa, Alessio Arbuatti, Vito Martella, Barbara Di Martino, and Federica Di Profio. 2022. "Detection and Characterization of a Novel Picornavirus in European Badger (Meles meles)" Veterinary Sciences 9, no. 11: 645. https://doi.org/10.3390/vetsci9110645