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Article

A Double Histochemical/Immunohistochemical Staining for the Identification of Canine Mast Cells in Light Microscopy

Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia, 40064 Bologna, Italy
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Author to whom correspondence should be addressed.
Academic Editors: Fernando Ferreira and Justina Prada
Vet. Sci. 2021, 8(10), 229; https://doi.org/10.3390/vetsci8100229
Received: 3 August 2021 / Revised: 14 September 2021 / Accepted: 10 October 2021 / Published: 12 October 2021
(This article belongs to the Special Issue Tumors in Pets: New Diagnostic and Therapeutic Tools)
Immunohistochemistry (IHC) is a widely used technique in diagnostic pathology, but the simultaneous analysis of more than one antibody at a time with different chromogens is rather complex, time-consuming, and quite expensive. In order to facilitate the identification of mast cells (MCs) during immunohistochemical analysis of membrane and/or nuclear markers, we propose a new staining method that includes the association of IHC and toluidine blue as a counterstain. To achieve this goal, we tested c-kit, Ki67, and cannabinoid receptor 2 on several cases of cutaneous canine mast cell tumors (MCTs), cutaneous mastocytosis, and atopic dermatitis. The results obtained show how this double staining technique, although limited to non-cytoplasmic markers and of little use in poorly differentiated MCTs in which MC metachromasia is hard to see, can be used during the evaluation of nuclear and/or membranous immunohistochemical markers in all canine cutaneous disorders, especially if characterized by the presence of a low number of MCs. It can help to evaluate those MCTs in which neoplastic MCs must be clearly distinguished from inflammatory cells that can infiltrate the tumor itself, in facilitating the calculation of the Ki67 index. Moreover, it can be used to study the expression of new markers in both animal and human tissues containing MCs and in MC disorders. View Full-Text
Keywords: canine mast cell tumors; co-localization; immunohistochemistry; toluidine blue stain canine mast cell tumors; co-localization; immunohistochemistry; toluidine blue stain
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MDPI and ACS Style

Gobbo, F.; Sarli, G.; De Silva, M.; Galiazzo, G.; Chiocchetti, R.; Morini, M. A Double Histochemical/Immunohistochemical Staining for the Identification of Canine Mast Cells in Light Microscopy. Vet. Sci. 2021, 8, 229. https://doi.org/10.3390/vetsci8100229

AMA Style

Gobbo F, Sarli G, De Silva M, Galiazzo G, Chiocchetti R, Morini M. A Double Histochemical/Immunohistochemical Staining for the Identification of Canine Mast Cells in Light Microscopy. Veterinary Sciences. 2021; 8(10):229. https://doi.org/10.3390/vetsci8100229

Chicago/Turabian Style

Gobbo, Francesca, Giuseppe Sarli, Margherita De Silva, Giorgia Galiazzo, Roberto Chiocchetti, and Maria Morini. 2021. "A Double Histochemical/Immunohistochemical Staining for the Identification of Canine Mast Cells in Light Microscopy" Veterinary Sciences 8, no. 10: 229. https://doi.org/10.3390/vetsci8100229

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