Deciphering the Role of Bovine Viral Diarrhea Virus Non-Structural NS4B Protein in Viral Pathogenesis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal and Ethical Statement
2.2. Samples
2.3. Cells and Viruses
2.4. NS4B Expression in Mammalian Cells
2.5. Virus Neutralization Assay (VNA)
2.6. SDS-PAGE and Western Blot
2.7. Indirect ELISA
2.8. Statistical Analysis
3. Results
3.1. BVDV Vaccination Elicits a Potent Post-Immunization Antibody Response
3.2. Antigenic Evaluation of NS4B Using Western Blot and ELISA
4. Discussion
5. Conclusions and Perspectives
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Cow Number | Pre-Immune Neutralization Titre Dilutions * | Post-Immune Neutralization Titre Dilutions * |
---|---|---|
1 | - | 1:10,240 |
2 | - | 1:10,240 |
3 | 1:10,240 | 1:10,240 |
4 | - | 1:2560 |
5 | 1:5120 | 1:5120 |
Animal | Status | VNA | W.B | ELISA |
---|---|---|---|---|
Cow 1 | Pre-Immunization | − | − | − |
Post-immunization | + | − | − | |
Cow 2 | Pre-Immunization | − | − | − |
Post-immunization | + | − | − | |
Cow3 | Pre-Immunization | + | + | + |
Post-immunization | + | + | + | |
Cow4 | Pre-Immunization | − | − | − |
Post-immunization | + | + | − | |
Cow5 | Pre-Immunization | + | + | + |
Post-immunization | + | + | + |
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Bashir, S.; Kossarev, A.; Martin, V.C.; Paeshuyse, J. Deciphering the Role of Bovine Viral Diarrhea Virus Non-Structural NS4B Protein in Viral Pathogenesis. Vet. Sci. 2020, 7, 169. https://doi.org/10.3390/vetsci7040169
Bashir S, Kossarev A, Martin VC, Paeshuyse J. Deciphering the Role of Bovine Viral Diarrhea Virus Non-Structural NS4B Protein in Viral Pathogenesis. Veterinary Sciences. 2020; 7(4):169. https://doi.org/10.3390/vetsci7040169
Chicago/Turabian StyleBashir, Shahbaz, Andrey Kossarev, Violeta Cascon Martin, and Jan Paeshuyse. 2020. "Deciphering the Role of Bovine Viral Diarrhea Virus Non-Structural NS4B Protein in Viral Pathogenesis" Veterinary Sciences 7, no. 4: 169. https://doi.org/10.3390/vetsci7040169