Next Article in Journal
Urinary Fractional Excretion of Phosphorus in Dogs with Spontaneous Chronic Kidney Disease
Previous Article in Journal
Risk Factors Associated with Brucella Seropositivity in Sheep and Goats in Duhok Province, Iraq
Article Menu
Issue 4 (December) cover image

Export Article

Open AccessArticle
Vet. Sci. 2017, 4(4), 66;

Histology of the Ovary of the Laying Hen (Gallus domesticus)

College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA
Department of Animal and Rangeland Sciences, Oregon State University, Corvallis, OR 97331, USA
Author to whom correspondence should be addressed.
Academic Editor: Patrick Butaye
Received: 5 October 2017 / Revised: 29 November 2017 / Accepted: 5 December 2017 / Published: 11 December 2017
Full-Text   |   PDF [14032 KB, uploaded 11 December 2017]   |  


The laying hen (Gallus domesticus) is a robust animal model for epithelial ovarian cancer. The use of animal models is critical in identifying early disease markers and developing and testing chemotherapies. We describe the microscopic characteristics of the normally functioning laying hen ovary and proximal oviduct to establish baselines from which lesions associated with ovarian cancer can be more readily identified. Ovaries and oviducts were collected from 18-month-old laying hens (n = 18) and fixed in 10% neutral buffered formalin. Hematoxylin- and eosin-stained sections were examined by light microscopy. Both post-ovulatory follicular regression and atresia of small follicles produce remnant clusters of vacuolated cells with no histological evidence that scar tissue persists. Infiltrates of heterophils are associated with atresia of small follicles, a relationship not previously documented in laying hen ovaries. Because these tissues can be mistaken for cancerous lesions, we present a detailed histological description of remnant Wolffian tissues in the laying hen ovary. Immunohistochemical staining for pancytokeratin produced a positive response in ovarian surface epithelium and staining for vimentin produced a positive response in granulosa cells of follicles. Epithelial cells lining glands of the remnant epoöphoron had a positive response to both pancytokeratin and vimentin, a result also observed in women. View Full-Text
Keywords: laying hen; epithelial ovarian cancer; histology; rete ovarii; epoöphoron; ovarian surface epithelium laying hen; epithelial ovarian cancer; histology; rete ovarii; epoöphoron; ovarian surface epithelium

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Apperson, K.D.; Bird, K.E.; Cherian, G.; Löhr, C.V. Histology of the Ovary of the Laying Hen (Gallus domesticus). Vet. Sci. 2017, 4, 66.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Vet. Sci. EISSN 2306-7381 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top