Stimulation with Concanavalin-A Induces IL-17 Production by Canine Peripheral T Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Mitogen-Stimulation Promotes IL-17 Production by Canine CD4 and CD8 T Cells
%IL-17-Producing Cells in ConA Blasts (6 h) | ||
---|---|---|
Dog # | CD4 | CD8 |
1 | 12.4 | 17.6 |
2 | 4.5 | 4.8 |
3 | 11 | 5.2 |
4 | 1.0 | 8.8 |
2.2. Pro-Inflammatory Cytokines Promote Polarization of Canine T Helper Cells towards the Th17 Phenotype
Condition | Dog-1 | Dog-2 |
IL-17 (±S. D.; ng/mL) | ||
Unstimulated | 0 (0) | 4.5 (0.8) |
ConA | 8.3 (0.3) | 8.4 (1.7) |
ConA+TGF-ß+IL-2 | 8.4 (1.0) | 9.8 (0.4) |
ConA+TGF-ß+IL-1ß+IL-6 | 9.5 (2.7) | 9.4 (0.3) |
3. Materials and Methods
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ritt, M.G.; Lindborg, B.A.; O'Brien, T.D.; Bisignano, J.; Modiano, J.F. Stimulation with Concanavalin-A Induces IL-17 Production by Canine Peripheral T Cells. Vet. Sci. 2015, 2, 43-51. https://doi.org/10.3390/vetsci2020043
Ritt MG, Lindborg BA, O'Brien TD, Bisignano J, Modiano JF. Stimulation with Concanavalin-A Induces IL-17 Production by Canine Peripheral T Cells. Veterinary Sciences. 2015; 2(2):43-51. https://doi.org/10.3390/vetsci2020043
Chicago/Turabian StyleRitt, Michelle G., Beth A. Lindborg, Timothy D. O'Brien, Joseph Bisignano, and Jaime F. Modiano. 2015. "Stimulation with Concanavalin-A Induces IL-17 Production by Canine Peripheral T Cells" Veterinary Sciences 2, no. 2: 43-51. https://doi.org/10.3390/vetsci2020043