The Very Virulent IBDV Viral Protein VP3 Promotes the Caspase-3 Mediated Cleavage of GSDME
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Plasmid Construction and Transfection
2.3. Antibodies and Reagents
2.4. Co-Immunoprecipitation (Co-IP) and Western Blotting
2.5. Confocal Microscopy
2.6. RT-qPCR
2.7. Ethics Statement
2.8. Animal Experiment
2.9. Lactate Dehydrogenase Release Assay
2.10. Flow Cytometric Analysis of Cell Death Using PI
2.11. Statistical Analysis
3. Results
3.1. The Transcriptional Levels of GSDME and IL-1β Were Positively Correlated in the Tissues Where vvIBDV Replicated
3.2. IBDV Proteins VP2, VP3, and VP4 Interact with GSDME but Cannot Directly Cleave It
3.3. Chicken Caspase-3 Specifically Cleaves GSDME at Position D270, Thereby Inducing Pyroptosis
3.4. Viral Protein VP3 of vvIBDV Enhances Caspase-3-Mediated Cleavage of GSDME
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhang, T.; Wang, S.; Qi, X.; Tang, L.; Gao, Y. The Very Virulent IBDV Viral Protein VP3 Promotes the Caspase-3 Mediated Cleavage of GSDME. Vet. Sci. 2026, 13, 373. https://doi.org/10.3390/vetsci13040373
Zhang T, Wang S, Qi X, Tang L, Gao Y. The Very Virulent IBDV Viral Protein VP3 Promotes the Caspase-3 Mediated Cleavage of GSDME. Veterinary Sciences. 2026; 13(4):373. https://doi.org/10.3390/vetsci13040373
Chicago/Turabian StyleZhang, Tao, Suyan Wang, Xiaole Qi, Lijie Tang, and Yulong Gao. 2026. "The Very Virulent IBDV Viral Protein VP3 Promotes the Caspase-3 Mediated Cleavage of GSDME" Veterinary Sciences 13, no. 4: 373. https://doi.org/10.3390/vetsci13040373
APA StyleZhang, T., Wang, S., Qi, X., Tang, L., & Gao, Y. (2026). The Very Virulent IBDV Viral Protein VP3 Promotes the Caspase-3 Mediated Cleavage of GSDME. Veterinary Sciences, 13(4), 373. https://doi.org/10.3390/vetsci13040373

