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Case Report

A Case of Starch Overload in Young Dairy Heifers: A Physiological and Nutritional Point of View

1
Department of Veterinary Sciences, University of Turin, Largo P. Braccin 2, 10095 Grugliasco, Torino, Italy
2
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia =, Bologna, Italy
3
Department of Veterinary Sciences, University of Teramo, SP18, 64100 Teramo, Italy
*
Author to whom correspondence should be addressed.
Vet. Sci. 2026, 13(4), 319; https://doi.org/10.3390/vetsci13040319
Submission received: 19 February 2026 / Revised: 23 March 2026 / Accepted: 25 March 2026 / Published: 26 March 2026
(This article belongs to the Section Nutritional and Metabolic Diseases in Veterinary Medicine)

Simple Summary

To become healthy and productive cows, dairy heifers need to grow at the right pace. However, excessive intake of concentrate feeds rich in starch can lead to digestive problems and excessive weight gain. This case report describes a group of young dairy heifers on a commercial farm showing loose manure and unusually rapid growth associated with high concentrate intake. On-farm monitoring and laboratory analyses suggested that excessive starch intake contributed to digestive imbalance. When concentrate allowance was restricted and forage intake increased, fecal consistency improved and growth rate stabilized. These observations highlight the importance of balancing concentrate and forage in heifer diets and of monitoring growth and fecal characteristics to detect nutritional imbalances early. Proper feeding management during this stage can help support digestive health, prevent excessive fat deposition, and promote better long-term productivity and animal welfare.

Abstract

In order to guarantee sufficient growth, digestive stability, and long-term productivity in dairy heifers, proper nutritional management is crucial both before and after weaning. This case study assesses the impact of dietary modifications on growth performance and digestive parameters in commercial settings and details a field observation of concentrate overload in young Holstein heifers. From 77 to 165 days of age, the body weight (BW), average daily gain (ADG), body condition score (BCS), feed intake, and fecal characteristics of 15 calves were monitored. Infectious and parasitic causes of diarrhea were ruled out by fecal examinations. Ad libitum concentrate feeding resulted in low fecal scores with undigested grain particles and acidic smell, starch intake exceeding requirements, and concentrate intake reaching up to 6 kg as fed head×day. The BCS gradually rose, and ADG peaked at 1.64 kg/day. Forage intake increased, fecal consistency improved, and ADG stabilized after restricting concentrate allowance to 2.5% of BW. These results underline the significance of controlling starch intake and concentrate allowance to avoid excessive growth and digestive disorders in developing dairy heifers, and they support a nutritional basis for the observed digestive imbalance.

1. Introduction

The management of dairy calves during the early stages of life represents a critical component of replacement heifer production systems, both from an economic and biological perspective. Feeding strategies implemented during the pre-weaning and immediate post-weaning phases have long-term implications for growth performance, metabolic health, mammary development, and future milk yield [1,2]. Because of this, the importance of calf nutrition in determining lifetime productivity and farm profitability is becoming more widely acknowledged.
During the first few weeks of life, newborn calves, which are functionally monogastric animals, mainly rely on milk digestion. The development of rumen structure, microbial colonization, and metabolic adaptation to solid feed intake are all part of the slow and intricate process of becoming a functional ruminant [3]. The introduction of starter feed, which promotes microbial fermentation and the production of volatile fatty acids (VFAs), especially butyrate and propionate, which support rumen epithelial development, is what causes this shift [4,5,6]. At the same time, adequate physical fiber intake plays a fundamental role in supporting rumen motility and muscular development, while helping to regulate fermentation patterns. The balance between fermentable carbohydrates and structural fiber is therefore essential to ensure proper rumen maturation and digestive stability. If this balance is disrupted, especially through excessive intake of rapidly fermentable carbohydrates such as starch, the developing digestive system may not be able to adequately regulate fermentation processes [7].
Under practical farm conditions, management of replacement calves may receive less attention compared with lactating animals, as they do not generate immediate economic returns. However, suboptimal feeding strategies during this phase can have significant long-term consequences, including altered growth trajectories, excessive fat deposition, impaired mammary gland development, and reduced milk production in later life [8,9].
To prevent unintentional metabolic and developmental imbalances, it is crucial to closely monitor growth parameters and nutritional intake.
Although there are well-established guidelines for calf feeding and rumen development, there is still little evidence of field cases where young heifers raised in commercial settings experience noticeable physiological and digestive problems as a result of poor nutritional management. Specifically, detailed descriptions of the effects of uncontrolled concentrate intake during the transition from pre-ruminant to ruminant stages are rare. The aim of this case report is to describe a field-observed condition of concentrate overload in young dairy heifers and to evaluate its effects on growth performance, fecal characteristics, and indicators of digestive imbalance under commercial farm management.

2. Materials and Methods

2.1. Animals

The case was conducted in a commercial Holstein dairy herd located in northeast Italy, within the Parmigiano Reggiano cheese production area. The herd consisted of approximately 150 lactating cows. The farm was visited during a nutritional consultancy advised by the farm’s referring veterinarian.
A group of 15 pre-weaning female Holstein calves (future dairy heifers) was selected for monitoring based on the presence of abnormal growth patterns (above expected average daily gain) and altered fecal consistency (persistently low fecal scores and presence of undigested particles). Animals were selected during a veterinary nutritional consultation, and inclusion criteria were based on clinical observation of rapid growth, increased body condition score, and signs of digestive imbalance in the absence of evident infectious disease. Calves were housed in multiple concrete pens bedded with abundant straw. Weaning was routinely performed at approximately 3 months of age, according to farm management practices.
Before and after weaning, calves were fed ad libitum concentrate and hay. The high concentrate intake observed in this group was not part of a planned feeding strategy, but reflected routine farm management practices, where concentrate was offered ad libitum without adjustment for body weight or developmental stage. Milk allowance before weaning was maintained at 3 L head×day until nutritional interventions were applied. All animals were managed according to standard commercial practices, and no experimental procedures were imposed.

2.2. Feeds and Feed Analysis

During the initial evaluation, calves were fed a commercial concentrate ad libitum (Feed A) and first-cut hay produced on-farm. Feed A was composed of corn, hulled soybean meal, wheat bran, toasted soybeans, dried beet pulp, sugarcane molasses, soybean oil, calcium carbonate, dicalcium phosphate, and sodium chloride. The declared chemical composition included 20% crude protein (CP), 5% crude fiber (CF), 5% ether extract (EE), and 7% ash. Vitamin, mineral, and additive contents were provided by the manufacturer and included live yeast (Saccharomyces cerevisiae, 1 × 109 CFU).
During the monitoring period, two feeding management interventions were implemented. At approximately 113–119 days of age, concentrate (Feed A) was temporarily restricted to three meals of approximately 1 kg each. Later, from approximately 134 days of age onward, concentrate allowance was restricted to 2.5% of estimated body weight. Feed B consisted of corn, hulled sunflower meal, wheat bran, corn gluten meal, sunflower seed by-products, wheat middlings, hulled soybean meal, calcium carbonate, sugarcane molasses, and sodium chloride. The declared composition of Feed B included 19% CP, 10% CF, 4% EE, and 6.6% ash.
The chemical compositions of Feed A and B were determined through laboratory analysis, and the results are reported in Table 1. Nutrient composition of the first-cut hay is reported in Table 2.
All feed samples were analyzed for dry matter (DM), CP, EE, neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), ash, and starch using a TANGO FT-NIR spectrometer (Bruker Optics GmbH, Ettlingen, Germany), according to previous reports [10,11].

2.3. Animal Measurement and Monitoring

Calves were monitored weekly by the same trained operator throughout the pre- and post-weaning period, from the day of inclusion (77 days old) to 165 days of life (11 monitoring time points), to ensure consistency in data collection. Body weight, wither height, and chest girth were recorded to evaluate growth performance and body development. Body weight (BW) was measured using a calibrated electronic scale, while wither height and chest girth were measured using standard measuring tools and anatomical reference points to ensure repeatability. Average daily gain (ADG) was calculated for each monitoring interval.
Body condition score (BCS) was assessed by the same evaluator to minimize inter-observer variability, using a 5-point scale [12], to evaluate fat deposition and overall body condition.
Feed intake was estimated daily by recording the amount of feed offered and weighing refusals, and values were expressed as average intake per animal. Milk intake was recorded daily until weaning. Digestive function was evaluated by fecal consistency scoring [12] and by wet sieving of fecal samples, as described by [13], to assess the presence of undigested feed particles.
To exclude infectious and parasitic causes of diarrhea, individual fecal samples were collected directly from the rectum by the attending veterinarian using disposable gloves, ensuring minimal contamination. Samples were placed in sterile containers and analysed shortly after collection.
Coprological examinations were performed by a veterinary diagnostic laboratory using standard flotation techniques for the detection of coccidia and Cryptosporidium spp. In addition, commercial antigen detection tests were used to identify rotavirus, coronavirus, Cryptosporidium spp., and Escherichia coli F5 (K99), following the manufacturer’s instructions.
All monitoring procedures were conducted under routine farm conditions without experimental manipulation, ensuring that observations reflected real-world management practices.

3. Results

3.1. Baseline Conditions and Initial Clinical Findings

At the beginning of the monitoring period (77 ± 2 days of life), the 15 pre-weaning calves had an average body weight of 101 kg, with a wither height of 92 cm and a chest girth of 110 cm (Table 3). Milk intake was 3 L head×day. The average fecal score was 1.5, indicating loose fecal consistency (Table 4).

3.2. Growth Performance and Body Condition Development

Throughout the monitoring period, calves exhibited a rapid growth rate. Average daily gain ranged from 0.92 to 1.64 kg/day depending on the interval between consecutive monitoring measurements, with progressive increases in body weight, average daily gain and BCS (Table 4).
BCS increased over time, reaching values close to or exceeding 2.8 during the later stages of the monitoring period, particularly during phases characterized by high concentrate intake.

3.3. Feed Intake Patterns and Dietary Changes

Concentrate intake progressively increased, reaching approximately 3.4–3.8 kg as fed head×day, whereas forage intake remained limited (Table 4).
At approximately 113–119 days of age, a temporary restriction of Feed A to three meals of approximately 1 kg each was applied. This intervention resulted in increased forage intake and an improvement in fecal score, whereas milk reduction and subsequent weaning did not produce appreciable changes in fecal consistency.
At approximately 123–133 days of age, Feed A was replaced by Feed B, which was initially offered ad libitum. During this phase, concentrate intake further increased, reaching values up to 6 kg as fed head×day, while forage intake markedly decreased.
From approximately 134 days of age onward, concentrate allowance was restricted to 2.5% of estimated BW. Following this intervention, concentrate intake decreased to approximately 4.5–4.9 kg as fed head×day, and forage intake increased.

3.4. Fecal Characteristics and Digestive Findings

During the initial and intermediate phases of the monitoring period (77–113 days of age), characterized by ad libitum concentrate feeding, loose fecal consistency persisted, with fecal scores ≤ 1.5. Wet sieving performed during this phase revealed the presence of undigested corn particles and mucus in the feces.
During the temporary restriction of Feed A (approximately 113–119 days of age), fecal consistency showed a partial improvement.
During the subsequent phase in which Feed B was offered ad libitum (approximately 123–133 days of age), fecal consistency deteriorated again, and an acidic odor of the feces was noted.
From approximately 134 days of age onward, following restriction of concentrate allowance to 2.5% of estimated BW, fecal consistency progressively improved, with fecal scores increasing to values ≥ 2.5 and a greater proportion of fibrous material visually detectable in the feces.
A temporal association between fecal score and estimated dietary starch concentration across the monitoring period is reported in Table 5.

3.5. Health Screening

Coprological examination and antigenic testing were negative for coccidia, Cryptosporidium spp., rotavirus, coronavirus, and Escherichia coli F5 (K99).

4. Discussion

The present case report describes a condition of concentrate overload in young dairy heifers, and its relation to increased growth rate, persistently low fecal score, and impaired digestive efficiency in the absence of infectious or parasitic diseases. The temporal association between high concentrate intake, elevated dietary starch content, and altered fecal characteristics strongly supports a nutritional origin of the observed disorder [7]. High levels of concentrate supplementation can alter feeding behavior and reduce forage intake due to substitution effects and changes in ruminal fermentation dynamics [14]. However, nutritional strategies based on concentrate restriction must also be carefully balanced to ensure adequate energy supply and maintain appropriate growth rates: maintaining an adequate forage-to-concentrate ratio represents key management strategies to support digestive stability and optimal development of replacement heifers [15].
In this case, weaning was performed at approximately three months of age, in accordance with the farm’s routine management. Although age-based weaning strategies are commonly adopted, optimal weaning management should consider additional parameters such as body weight and concentrate intake [16], adjusted based on breed and environmental factors. For large-breed calves, a minimum intake of approximately 0.9 kg of concentrate DM for at least three consecutive days has been recommended as a criterion for weaning [3]. In the present case, weaning decisions were driven primarily by age, and this may have contributed to an early exposure of the calves to high levels of fermentable carbohydrates.
When the present data were compared with available reference values, BW and concentrate intake, between approximately 11 and 20 weeks of age, were within the ranges reported for Holstein heifers. However, differences emerged when growth dynamics were evaluated over time. According to some conservative growth targets proposed [17], a BW of approximately 175 kg at six months of age is recommended to limit excessive prepubertal fat deposition. In the present case, this threshold was reached and exceeded before six months of age, with heifers weighing approximately 200 kg at around 5.5 months of age.
From a nutritional standpoint, NRC recommendations [18] provide a broader target BW of approximately 200 kg for Holstein heifers between 3 and 6 months of age, which was also reached earlier than expected in this case. In addition, ADG during the late prepubertal phase markedly exceeded commonly recommended values (1.64 vs. approximately 1.0 kg/day), and dry matter intake at around six months of age (7.4 kg/day) was higher than reference values [17,18].
Similarly, BCS progressively increased, approaching values associated with early fat deposition. Excessive growth rates and over conditioning during the prepubertal phase have been associated with impaired mammary gland development and reduced future milk production, likely mediated by alterations in endocrine signaling and mammogenic hormone secretion [19]. Therefore, the progressive increase in BCS observed in this case raised long-term concerns beyond digestive health alone. These data suggest that the issue was not the absolute BW achieved, but rather the timing and velocity of growth, with target BW being reached earlier than recommended during the prepubertal phase. From a physiological perspective, accelerated growth combined with increased BCS is associated with enhanced adipose tissue deposition within the mammary gland, which may compromise the development of functional parenchymal tissue. This process can negatively affect future milk production potential, as excessive fat accumulation during the prepubertal period has been linked to reduced mammary secretory capacity. A consistent relationship between dietary starch intake and fecal score was observed throughout the monitoring period. Low fecal scores and the presence of mucus and undigested grain particles were recorded during phases characterized by ad libitum concentrate feeding and estimated dietary starch levels exceeding 20% DM. In contrast, fecal consistency progressively normalized following concentrate restriction, when dietary starch content fell within recommended ranges. These findings are consistent with nutritional guidelines indicating optimal starch concentrations between 15 and 20% DM for growing calves and heifers, as reported in Table 6 [18].
From a physiological perspective, excessive intake of rapidly fermentable carbohydrates during the transition from pre-ruminant to functional ruminant status may overwhelm the adaptive capacity of both the rumen and the hindgut. In calves that are still developing ruminal absorptive and buffering mechanisms, high starch intake can increase the rate of fermentation and volatile fatty acid production beyond epithelial absorption capacity, leading to a decline in ruminal pH [20,21]. The source and fermentability of dietary starch can markedly influence rumen fermentation patterns, rumination activity, and overall digestive stability in growing ruminants. Differences in starch degradability among feed ingredients may also affect chewing activity and rumination behavior. Highly fermentable starch sources may increase the risk of fermentation imbalances when not adequately balanced with dietary fiber, highlighting the importance of controlling starch supply and its fermentability in heifer feeding strategies [22].
In pre-ruminant or early-transition calves, the intestinal mucosa is not fully adapted to large post-ruminal starch loads. Excess substrate reaching the hindgut undergoes rapid microbial fermentation, resulting in lactic acid accumulation, osmotic water influx, and increased luminal acidity, contributing to nutritional diarrhea through osmotic imbalance and mucosal irritation [23].
Moreover, both ruminal and hindgut acidosis have been associated with epithelial damage, inflammation, and translocation of lipopolysaccharides, contributing to systemic health challenges [24]. Although the precise site of acidosis could not be determined under commercial farm conditions [25], the acidic odor of feces and the marked improvement following concentrate restriction are consistent with carbohydrate-induced digestive acidosis [26].
In this context, fecal evaluation proved to be a practical and informative on-farm diagnostic tool: changes in fecal consistency, odor, and particle size composition closely mirrored dietary modifications and provided immediate feedback on digestive balance. Low fecal scores were observed exclusively during periods of high starch intake, whereas optimal fecal consistency was achieved only after concentrate allowance was limited and forage intake increased. These observations highlight the value of routine fecal assessment, where fecal particle size confirms its utility as a mirror of a healthy gastrointestinal tract and indicator of ration adequacy in growing heifers; in this context, routine fecal evaluation may represent an early warning tool, allowing practitioners to identify excessive fermentable carbohydrate intake before overt metabolic or developmental consequences become evident.
This study has several limitations, including the absence of a control group and the limited number of animals enrolled, restricting the generalizability of the findings. However, the present work was designed as a field case report conducted during a veterinary nutritional consultation under commercial farm conditions, and therefore, the inclusion of a control group was not feasible.
Overall, this case emphasizes the importance of regulating concentrate intake in young dairy heifers to prevent digestive disorders, excessive growth rates, and over conditioning during critical developmental stages. Under commercial conditions, concentrate restriction based on BW proved to be an effective and practical approach to restoring digestive balance and controlling growth rate in replacement heifers [15]. As technology continues to advance, precision livestock farming (PLF) systems are increasingly being implemented to support dairy farmers, technicians, and veterinarians in the accurate quantification and evaluation of management parameters [27,28]. From a broader herd management perspective, the condition described in this case reflects a mismatch between nutritional supply and the physiological capacity of the developing animal, which may not be immediately evident under routine farm conditions. Early detection of such imbalances relies heavily on the systematic monitoring of key indicators, including growth rate, body condition score, feed intake patterns, and fecal characteristics. In this context, the integration of objective and continuous monitoring approaches could enhance the ability to identify subtle deviations from optimal developmental trajectories. Precision Livestock Farming (PLF) technologies, which enable the collection of real-time behavioral and physiological data, may represent a valuable complementary tool to traditional on-farm assessments, particularly in supporting early warning systems for nutritional and metabolic disorders. However, their application in young heifers remains limited, and further research is needed to validate their effectiveness and practical implementation under commercial conditions [29,30].

5. Conclusions

This case report highlights how excessive concentrate intake and elevated dietary starch levels can impair digestive function and promote excessive growth in young dairy heifers under commercial conditions. Nutritional diarrhea associated with concentrate overload can be effectively prevented through a limited number of complementary strategies, including reducing total starch intake and the use of less rapidly fermentable carbohydrate sources, together with the promotion of adequate roughage intake through ration formulation or concentrate restriction.
When formulating rations for growing heifers, the primary objective should be to achieve appropriate BW targets while avoiding overconditioning during critical developmental stages. Regular monitoring of body weight, wither height, chest girth, and BCS is essential to assess growth dynamics, while routine evaluation of fecal consistency provides a practical and effective on-farm tool to detect dietary imbalances. Together, these approaches allow timely nutritional adjustments that support both digestive health and optimal long-term performance of replacement heifers.

Author Contributions

Conceptualization, T.D. and D.C.; methodology, D.C., M.L. and R.C.; software, M.L.; formal analysis, R.C., G.B. and M.L.; investigation, D.C.; data curation, D.C.; writing—original draft preparation, T.D. and M.L.; writing—review and editing, I.F., G.B., D.C. and E.V.; visualization, G.B. and R.C.; supervision, I.F. and E.V. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

The present study reports observations collected during routine veterinary clinical activity and nutritional consultancy on a commercial dairy farm. No experimental procedures were performed on the animals. According to Italian legislation (DL No. 26 of 4 March 2014), ethical committee approval is not required for observational studies or routine diagnostic procedures carried out within standard veterinary practice.

Informed Consent Statement

Not applicable.

Data Availability Statement

The original contributions presented in this study are included in the article. Further inquiries can be directed to the corresponding author(s).

Conflicts of Interest

The authors declare no conflicts of interest.

References

  1. Marliani, G.; Lamanna, M.; Buonaiuto, G.; Cavallini, D.; Accorsi, P.A. Coping styles in Holstein Heifers: Relationship between proactivity and habituation to automatic milking system. Biosyst. Eng. 2026, 263, 104394. [Google Scholar] [CrossRef]
  2. Soberon, F.; Raffrenato, E.; Everett, R.W.; Van Amburgh, M.E. Preweaning milk replacer intake and effects on long-term productivity of dairy calves. J. Dairy Sci. 2012, 95, 783–793. [Google Scholar] [CrossRef]
  3. Drackley, J.K. Calf Nutrition from Birth to Breeding. Vet. Clin. N. Am. Food Anim. Pract. 2008, 24, 55–86. [Google Scholar] [CrossRef]
  4. Palczynski, L.J.; Bleach, E.C.L.; Brennan, M.L.; Robinson, P.A. Appropriate Dairy Calf Feeding from Birth to Weaning: “It’s an Investment for the Future”. Animals 2020, 10, 116. [Google Scholar] [CrossRef]
  5. Mortazavi, M.S.; Hajmohammadi, M.; Buonaiuto, G.; Colleluori, R.; Lamanna, M.; Cavallini, D.; Valizadeh, R.; Ebrahimi, S.H.; Oliveira, C.A.F. The Effect of a Pre-Mix of Essential Organic Minerals on Growth, Antioxidant Indices, and the Diarrhea Incidence in Dairy Calves Breed in Arid Climates. Ruminants 2025, 5, 22. [Google Scholar] [CrossRef]
  6. Zanton, G.I.; Heinrichs, A.J. Meta-Analysis to Assess Effect of Prepubertal Average Daily Gain of Holstein Heifers on First-Lactation Production. J. Dairy Sci. 2005, 88, 3860–3867. [Google Scholar] [CrossRef] [PubMed]
  7. Humer, E.; Petri, R.; Aschenbach, J.; Bradford, B.; Penner, G.; Tafaj, M.; Südekum, K.-H.; Zebeli, Q. Invited review: Practical feeding management recommendations to mitigate the risk of subacute ruminal acidosis in dairy cattle. J. Dairy. Sci. 2018, 101, 872–888. [Google Scholar] [CrossRef]
  8. Rashid, H.U.; Khan, T.; Turi, A.A.; Abbas, M.; Ullah, K.; Fusaro, I.; Buonaiuto, G.; Cavallini, D. Impact of urea-molasses treated wheat straw levels in total mixed rations on growth and nutrient digestibility in Azikheli buffalo calves. Trop. Anim. Health Prod. 2025, 57, 110. [Google Scholar] [CrossRef] [PubMed]
  9. Cavallini, D.; Raspa, F.; Marliani, G.; Nannoni, E.; Martelli, G.; Sardi, L.; Valle, E.; Pollesel, M.; Tassinari, M.; Buonaiuto, G. Growth Performance and Feed Intake Assessment of Italian Holstein Calves Fed a Hay-Based Total Mixed Ration: Preliminary Steps towards a Prediction Model. Vet. Sci. 2023, 10, 554. [Google Scholar] [CrossRef]
  10. Buonaiuto, G.; Cavallini, D.; Mammi, L.M.E.; Ghiaccio, F.; Palmonari, A.; Formigoni, A.; Visentin, G. The accuracy of NIRS in predicting chemical composition and fibre digestibility of hay-based total mixed rations. Ital. J. Anim. Sci. 2021, 20, 1730–1739. [Google Scholar] [CrossRef]
  11. Cavallini, D.; Mammi, L.M.E.; Fustini, M.; Palmonari, A.; Heinrichs, A.J.; Formigoni, A. Effects of ad libitum or restricted access to total mixed ration with supplemental long hay on production, intake, and rumination. J. Dairy Sci. 2018, 101, 10922–10928. [Google Scholar] [CrossRef] [PubMed]
  12. Hulsen, J. Cow Signals: A Practical Guide for Dairy Farm Management; RoodBond Publishers: Zutphen, The Netherlands, 2011; p. 96. [Google Scholar]
  13. Kljak, K.; Heinrichs, B.S.; Heinrichs, A.J. Fecal particle dry matter and fiber distribution of heifers fed ad libitum and restricted with low and high forage quality. J. Dairy Sci. 2019, 102, 4694–4703. [Google Scholar] [CrossRef] [PubMed]
  14. Scaglia, G. Supplementation of Growing Beef Heifers With Starch or Highly Digestible Fiber Supplements. J. Agric. Sci. 2020, 12, 14. [Google Scholar] [CrossRef]
  15. Lascano, G.J.; Zanton, G.I.; Suarez-Mena, F.X.; Heinrichs, A.J. Effect of limit feeding high- and low-concentrate diets with Saccharomyces cerevisiae on digestibility and on dairy heifer growth and first-lactation performance. J. Dairy Sci. 2009, 92, 5100–5110. [Google Scholar] [CrossRef]
  16. Xiao, J.; Ma, H.; Tian, J.; Lu, G.; Wei, X.; Peng, Y.; Hu, R.; Peng, Q.; Wang, L.; Xue, B.; et al. Effects of increased milk allowance through within-study comparisons on dairy calf growth and future performance. J. Dairy Sci. 2025, 108, 10812–10836. [Google Scholar] [CrossRef]
  17. Moran, J. Calf Rearing: A Practical Guide; CSIRO Publishing: Clayton, Australia, 2002; p. 211. [Google Scholar]
  18. National Academies of Sciences, Engineering, and Medicine; Division on Earth and Life Studies; Board on Agriculture and Natural Resources; Committee on Nutrient Requirements of Dairy Cattle. Nutrient Requirements of Dairy Cattle; National Academies Press: Washington, DC, USA, 2021. [Google Scholar] [CrossRef]
  19. Meyer, M.J.; Capuco, A.V.; Ross, D.A.; Lintault, L.M.; Van Amburgh, M.E. Developmental and nutritional regulation of the prepubertal heifer mammary gland: I. Parenchyma and fat pad mass and composition. J. Dairy Sci. 2006, 89, 4289–4297. [Google Scholar] [CrossRef] [PubMed]
  20. Cavallini, D.; Mammi, L.M.E.; Biagi, G.; Fusaro, I.; Giammarco, M.; Formigoni, A.; Palmonari, A. Effects of 00-rapeseed meal inclusion in Parmigiano Reggiano hay-based ration on dairy cows’ production, reticular pH and fibre digestibility. Ital. J. Anim. Sci. 2021, 20, 295–303. [Google Scholar] [CrossRef]
  21. Laarman, A.H.; Sugino, T.; Oba, M. Effects of starch content of calf starter on growth and rumen pH in Holstein calves during the weaning transition. J. Dairy Sci. 2012, 95, 4478–4487. [Google Scholar] [CrossRef]
  22. Shipandeni, M.N.T.; Paula, E.M.; Esposito, G.; Faciola, A.P.; Raffrenato, E. Effects of starch sources varying in particle sizes on ruminal fermentation, nutrient flow, starch digestibility, and lactation performance of dairy cows. J. Anim. Sci. 2023, 101, skad147. [Google Scholar] [CrossRef]
  23. Cronin, S.; Smith, M.; Bradley, C.; Daley, V.; Gadeyne, F.; Bustos, M.; Gressley, T. Evaluation of hindgut buffers under high-starch diet conditions in lactating Holstein cows. J. Dairy Sci. 2025, 108, 4946–4962. [Google Scholar] [CrossRef]
  24. Monteiro, H.F.; Faciola, A.P. Ruminal acidosis, bacterial changes, and lipopolysaccharides. J. Anim. Sci. 2020, 98, skaa248. [Google Scholar] [CrossRef] [PubMed]
  25. Cavallini, D.; Mammi, L.M.E.; Buonaiuto, G.; Palmonari, A.; Valle, E.; Formigoni, A. Immune-metabolic-inflammatory markers in Holstein cows exposed to a nutritional and environmental stressing challenge. J. Anim. Physiol. Anim. Nutr. 2021, 105, 42–55. [Google Scholar] [CrossRef]
  26. Owens, F.N.; Secrist, D.S.; Hill, W.J.; Gill, D.R. Acidosis in cattle: A review. J. Anim. Sci. 1998, 76, 275–286. [Google Scholar] [CrossRef]
  27. Lamanna, M.; Bovo, M.; Cavallini, D. Wearable Collar Technologies for Dairy Cows: A Systematized Review of the Current Applications and Future Innovations in Precision Livestock Farming. Animals 2025, 15, 458. [Google Scholar] [CrossRef] [PubMed]
  28. Cavallini, D.; Giammarco, M.; Buonaiuto, G.; Vignola, G.; Vettori, J.D.M.; Lamanna, M.; Prasinou, P.; Colleluori, R.; Formigoni, A.; Fusaro, I. Two years of precision livestock management: Harnessing ear tag device behavioral data for pregnancy detection in free-range dairy cattle on silage/hay-mix ration. Front. Anim. Sci. 2025, 6, 1547395. [Google Scholar] [CrossRef]
  29. Giannone, C.; Sahraeibelverdy, M.; Lamanna, M.; Cavallini, D.; Formigoni, A.; Tassinari, P.; Torreggiani, D.; Bovo, M. Automated dairy cow identification and feeding behaviour analysis using a computer vision model based on YOLOv8. Smart Agric. Technol. 2025, 12, 101304. [Google Scholar] [CrossRef]
  30. Lamanna, M.; Muca, E.; Giannone, C.; Bovo, M.; Boffo, F.; Romanzin, A.; Cavallini, D. Artificial intelligence meets dairy cow research: Large language model’s application in extracting daily time-activity budget data for a meta-analytical study. J. Dairy Sci. 2025, 108, 10203–10219. [Google Scholar] [CrossRef]
Table 1. Chemical composition of Feed A and Feed B.
Table 1. Chemical composition of Feed A and Feed B.
ItemFeed AFeed B
DM 1, % as fed87.0188.88
CP, %DM23.821.38
EE, %DM4.856.48
NDF, %DM16.2728.57
ADF, %DM7.5516.91
ADL, %DM1.334.24
Ash, %DM7.308.81
Starch, %DM33.9225.10
1 DM = dry matter, CP = crude protein, EE = ether extract, NDF = neutral detergent fiber, ADF = acid detergent fiber, ADL = acid detergent lignin.
Table 2. Chemical composition of the first cut hay.
Table 2. Chemical composition of the first cut hay.
Item 1First Cut Hay
DM, % as fed90.88
CP, % DM8.56
EE, % DM1.35
aNDFom, % DM64.18
ADF, % DM42.96
ADL, % DM6.69
Ash, % DM5.24
Sugars, % DM8.36
1 DM = dry matter, CP = crude protein, EE = ether extract, aNDFom = amylase-treated ash-corrected neutral detergent fiber with addition of sodium sulphite, ADF = acid detergent fiber, ADL = acid detergent lignin.
Table 3. Baseline age (days; d), wither height (cm), and chest girth (cm) of the monitored pre-weaning calves across the monitoring period.
Table 3. Baseline age (days; d), wither height (cm), and chest girth (cm) of the monitored pre-weaning calves across the monitoring period.
Days of Life (d)Wither Height (cm)Chest Girth (cm)
7792110
8493112
8995114
9196117
9898120
113104132
114104132
119105134
123106138
126106138
133107140
134108143
140108143
141109145
147110148
148111148
154112148
155112150
161113153
162113153
165113153
Table 4. Baseline age (days; d), body weight (kg), average daily gain (ADG; kg), body condition score (BCS), fecal score, milk, forage and concentrate intakes (l and kg DM, respectively) of the monitored pre-weaning calves across the monitoring period.
Table 4. Baseline age (days; d), body weight (kg), average daily gain (ADG; kg), body condition score (BCS), fecal score, milk, forage and concentrate intakes (l and kg DM, respectively) of the monitored pre-weaning calves across the monitoring period.
Days of Life
(d)
Body Weight
(kg)
ADG
(kg)
BCSFecal ScoreMilk Intake
(L)
Forage Intake
(kg DM)
Concentrate Intake
(kg DM)
771011.072.501.53.00.361.41
841091.072.501.53.00.361.41
891141.072.361.52.00.732.82
911161.072.361.50.00.732.82
981241.052.361.00.00.823.17
1131391.052.461.00.00.733.34
1141400.922.462.00.02.272.64
1191450.922.462.00.02.272.64
1231490.922.502.00.00.364.66
1261510.922.502.00.00.364.66
1331571.142.501.50.00.365.28
1341591.142.752.50.02.273.96
1401671.142.752.50.02.273.96
1411681.572.753.00.02.274.14
1471751.572.753.00.02.274.14
1481761.572.863.00.02.364.31
1541891.572.863.00.02.364.31
1551911.642.863.00.02.454.66
1612001.642.863.00.02.454.66
1622021.642.863.00.02.454.93
1652071.642.863.00.02.454.93
Table 5. Temporal association (days; d) between fecal score and estimated dietary starch concentration (% DM) across the monitoring period.
Table 5. Temporal association (days; d) between fecal score and estimated dietary starch concentration (% DM) across the monitoring period.
Days of Life (d)Fecal ScoreStarch Intake (%DM)
771.527
841.527
891.527
911.527
98127
113128
114218
119218
123223
126223
1331.523
1342.516
1402.516
141316
147316
148316
154316
155316
161316
162317
165317
Table 6. Nutrient concentrations (DM basis) requirements for Holstein cattle at varying stages of lactation and ages of maturity (adapted from [18]).
Table 6. Nutrient concentrations (DM basis) requirements for Holstein cattle at varying stages of lactation and ages of maturity (adapted from [18]).
Growing Calves and Heifers (Age, Days)
Parameter30 d100 d225 d350 d475 d600 d
BW (kg)65120230330420530
Growth rate (kg/d)0.70.70.90.80.70.9
Dry matter intake (kg/d)1.43.96.68.59.811.0
ME (MJ/kg)3.682.262.091.951.922.12
NE (MJ/kg)------
Rumen-degradable protein (%)-10.010.010.010.010.0
Rumen-undegradable protein (%)-6.64.42.61.72.7
Crude protein (%)21.016.614.412.611.712.7
Metabolizable protein (%)16.59.58.16.86.114.0
NDF min (%)-25–3325–3325–3325–3325–33
Forage NDF min (%)-19–2519–2519–2519–2519–25
Starch max (%)-15–2015–2015–2015–2015–20
Ca (%)0.590.780.580.440.370.39
P (%)0.450.320.260.210.180.19
Mg (%)0.150.140.120.120.120.10
K (%)1.000.510.520.540.560.60
Na (%)0.350.170.160.160.150.16
Cl (%)0.280.140.140.130.130.13
S (%)-0.200.200.200.200.20
DCAD (mEq/kg min)-3942455060
Cu (mg/kg)51616151517
Co (mg/kg)-0.200.200.200.200.20
I (mg/kg)0.780.690.580.540.530.54
Fe (mg/kg)906146322428
Mn (mg/kg)504944403843
Se (mg/kg)0.30.30.30.30.30.3
Zn (mg/kg)704741363435
Vitamin A (IU/kg)521833903829426546985288
Vitamin D (IU/kg)15189241044116312811442
Vitamin E (IU/kg)864956626877
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Danese, T.; Valle, E.; Lamanna, M.; Colleluori, R.; Buonaiuto, G.; Fusaro, I.; Cavallini, D. A Case of Starch Overload in Young Dairy Heifers: A Physiological and Nutritional Point of View. Vet. Sci. 2026, 13, 319. https://doi.org/10.3390/vetsci13040319

AMA Style

Danese T, Valle E, Lamanna M, Colleluori R, Buonaiuto G, Fusaro I, Cavallini D. A Case of Starch Overload in Young Dairy Heifers: A Physiological and Nutritional Point of View. Veterinary Sciences. 2026; 13(4):319. https://doi.org/10.3390/vetsci13040319

Chicago/Turabian Style

Danese, Tommaso, Emanuela Valle, Martina Lamanna, Riccardo Colleluori, Giovanni Buonaiuto, Isa Fusaro, and Damiano Cavallini. 2026. "A Case of Starch Overload in Young Dairy Heifers: A Physiological and Nutritional Point of View" Veterinary Sciences 13, no. 4: 319. https://doi.org/10.3390/vetsci13040319

APA Style

Danese, T., Valle, E., Lamanna, M., Colleluori, R., Buonaiuto, G., Fusaro, I., & Cavallini, D. (2026). A Case of Starch Overload in Young Dairy Heifers: A Physiological and Nutritional Point of View. Veterinary Sciences, 13(4), 319. https://doi.org/10.3390/vetsci13040319

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