Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents and Solutions
2.2. Rams and Sperm Collection
2.3. Sperm Samples Analyses
2.4. Sperm Freezing
2.5. Semen Thawing
2.6. Sperm Kinematic Analysis
2.7. Flow Cytometric Assay
2.8. Internal Thermal Gradient and Thermal Energy Absorption of Straws During Thawing
2.9. Statistical Analysis
3. Results
3.1. Sperm Kinematic Analysis
3.2. Flow Cytometry Assay
3.3. Internal Thermal Gradient and Thermal Energy Absorption of Insemination Doses
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ptáček, M.; Savvulidi, F.G.; LeBrun, C.; Janošíková, M.; Kenzhebaev, T.; Omashev, K.; Kulataev, B.; Malmakov, N. Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa. Vet. Sci. 2024, 11, 602. https://doi.org/10.3390/vetsci11120602
Ptáček M, Savvulidi FG, LeBrun C, Janošíková M, Kenzhebaev T, Omashev K, Kulataev B, Malmakov N. Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa. Veterinary Sciences. 2024; 11(12):602. https://doi.org/10.3390/vetsci11120602
Chicago/Turabian StylePtáček, Martin, Filipp Georgijevič Savvulidi, Christopher LeBrun, Martina Janošíková, Temirkhan Kenzhebaev, Kairly Omashev, Beybit Kulataev, and Nurlan Malmakov. 2024. "Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa" Veterinary Sciences 11, no. 12: 602. https://doi.org/10.3390/vetsci11120602
APA StylePtáček, M., Savvulidi, F. G., LeBrun, C., Janošíková, M., Kenzhebaev, T., Omashev, K., Kulataev, B., & Malmakov, N. (2024). Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa. Veterinary Sciences, 11(12), 602. https://doi.org/10.3390/vetsci11120602