Feline Hemotropic Mycoplasma Species of Apparently Healthy Domestic Cats in Konya Province of Türkiye
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sampling Location and Collection of Blood Samples from Cats
2.2. Preparation of Thin Blood Smear
2.3. Separation of Plasma and Blood
2.4. Genomic DNA Isolation
2.5. Molecular Detection of Mycoplasma Spp.
2.6. Sequence Analysis of Mycoplasma spp. 16S rRNA Gene
2.7. Statistical Analysis
3. Results
3.1. Microscopy and PCR
3.2. Phylogenetic Analysis
3.3. Risk Factor Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Districts | n | Breed (n) | Gender | Age | Status |
---|---|---|---|---|---|
Ahırlı | 3 | Crossbreed (1), Tabby (1), Scottish Fold (1) | 1 ♀ 2 ♂ | <1 y: 2 >1 y: 1 | 3 owned |
Akören | 2 | Tabby (2) | 1 ♀ 1 ♂ | <1 y: 0 >1 y: 2 | 2 owned |
Akşehir | 2 | Crossbreed (1), Exotic Shorthair (1) | 1 ♀ 1 ♂ | <1 y: 0 >1 y: 2 | 2 owned |
Altınekin | 2 | Crossbreed (1), Tabby (1) | 1 ♀ 1 ♂ | <1 y: 0 >1 y: 2 | 2 owned |
Beyşehir | 6 | British Shorthair (1), Crossbreed (1), Orange Tabby (1), Scottish Fold (1), Tabby (2) | 1 ♀ 5 ♂ | <1 y: 1 >1 y: 5 | 6 owned |
Bozkır | 6 | British Shorthair (2), Crossbreed (1), Tabby (3) | 2 ♀ 4 ♂ | <1 y: 0 >1 y: 6 | 6 owned |
Cihanbeyli | 4 | British Shorthair (2), Crossbreed (2) | 1 ♀ 3 ♂ | <1 y: 0 >1 y: 4 | 4 owned |
Çumra | 13 | British Shorthair (1), Crossbreed (6), Orange Tabby (1), Persian (1), Scottish Fold (1), Scottish Straight (1), Tabby (1), Van (1) | 5 ♀ 8 ♂ | <1 y: 5 >1 y: 8 | 13 owned |
Doğanhisar | 4 | British Shorthair (1), Crossbreed (2), Persian (1) | 4 ♀ | <1 y: 1 >1 y: 3 | 4 owned |
Ereğli | 9 | British Shorthair (2), Crossbreed (1), Orange Tabby (1), Tabby (5), | 3 ♀ 6 ♂ | <1 y: 2 >1 y: 7 | 9 owned |
Güneysınır | 1 | Tabby (1) | 1 ♂ | <1 y: 0 >1 y: 1 | 1 owned |
Hadim | 4 | Ankara (1), Crossbreed (2), Tabby (1) | 3 ♀ 1 ♂ | <1 y: 1 >1 y: 3 | 4 owned |
Halkapınar | 1 | Crossbreed (1) | 1 ♀ | <1 y: 0 >1 y: 1 | 1 owned |
Ilgın | 13 | Crossbreed (3), Norwegian Forest Cat (1), Tabby (9) | 5 ♀ 8 ♂ | <1 y: 1 >1 y: 12 | 13 owned |
Kadınhanı | 10 | Ankara (2), British Shorthair (1), Crossbreed (3), Persian (1), Tabby (2), Van (1) | 3 ♀ 7 ♂ | <1 y: 2 >1 y: 8 | 10 owned |
Karapınar | 4 | British Shorthair (1), Crossbreed (1), Tabby (2) | 2 ♀ 2 ♂ | <1 y: 1 >1 y: 3 | 4 owned |
Karatay | 64 | Ankara (2), British Longhair (3), British Shorthair (4), Crossbreed (25), Maine Coon (1), Orange Tabby (2), Persian (1), Scottish Fold (4), Smokin (1), Tabby (19), Van (2) | 31 ♀ 33 ♂ | <1 y: 11 >1 y: 53 | 64 owned |
Kulu | 2 | Scottish Fold (2) | 2 ♂ | <1 y: 0 >1 y: 2 | 2 owned |
Meram | 65 | Ankara (1), Bombay (1), British Shorthair (5), Crossbreed (17), Orange Tabby (1), Russian Blue (2), Scottish Fold (5), Scottish Shorthair (1), Scottish Straight (2), Somali (1), Tabby (27), Tuxedo (1), Van (1) | 30 ♀ 35 ♂ | <1 y: 7 >1 y: 58 | 65 owned |
Sarayönü | 6 | American Bobtail (1), Crossbreed (1), Scottish Fold (1), Tabby (3) | 3 ♀ 3 ♂ | <1 y: 1 >1 y: 5 | 6 owned |
Selçuklu | 149 | Ankara (6), Bombay (1), British Shorthair (6), Crossbreed (102), Exotic Shorthair (1), Persian (2), Scottish Fold (3), Siamese (1), Tabby (26), Van (1) | 81 ♀ 68 ♂ | <1 y: 20 >1 y: 129 | 65 owned 84 stray |
Seydişehir | 9 | British Shorthair (1), Crossbreed (4), Scottish Fold (1), Tabby (3) | 6 ♀ 3 ♂ | <1 y: 1 >1 y: 8 | 9 owned |
Taşkent | 5 | Crossbreed (1), Tabby (4) | 5 ♀ | <1 y: 1 >1 y: 4 | 5 owned |
Total | 384 | American Bobtail (1), Ankara (12), Bombay (2), British Longhair (3), British Shorthair (27), Exotic Shorthair (2), Persian (6), Maine Coon (1), Crossbreed (176), Norwegian Forest Cat (1), Russian Blue (2), Orange Tabby (6), Scottish Fold (19), Scottish Shorthair (1), Scottish Straight (3), Siamese (1), Smokin (1), Somali (1), Tabby (112), Tuxedo (1), Van (6) | 190 ♀ 194 ♂ | <1 y: 57 >1 y: 327 | 300 owned 84 stray |
Mycoplasma Spesies | Primer Sequence | Target Gene | Bp | References |
---|---|---|---|---|
Mycoplasma spp. | HBT-F, ATA CGG CCC ATA TTC CTA CG HBT-R, TGC TCC ACC ACT TGT TCA | 16s rRNA | 595 bp | [24] |
Mycoplasma hemofelis | Hf-F, 5′-ACG AAA GTC TGA TGG AGC AAT A-3′ Hf-R, 5′-ACG CCC AAT AAA TCC GRA TAA T-3′ | 16s rRNA | 170 bp | [25] |
Candidatus Mycoplasma hemominutum | Hf-F, 5′-ACG AAA GTC TGA TGG AGC AAT A-3′ Hf-R, 5′-ACG CCC AAT AAA TCC GRA TAA T-3′ | 16s rRNA | 193 bp | [25] |
Candidatus Mycoplasma turicensis | Mt1-F, 5′-GTATCCTCCATCAGACAGAA-3′ Mt2-R, 5′-CGCTCCATATTTAATTCCAA-3′ | 16s rRNA | 488 bp | [26] |
Mycoplasma hemocanis | Mhcf,5′-GAAACTAAGGCCATAAATGACGC-3′ Mhcr, 5′-ACCTGTCACCTCGATAACCTCTAC-3′ | 16s rRNA | 309 bp | [27] |
Candidatus Mycoplasma hematoparvum | CMhpf, 5′-ACGAAAGTCTGATGGAGCAATAC-3′ CMhpr, 5′-TATCTACGCATTCCACCGCTAC-3′ | 16s rRNA | 328 bp | [27] |
Mycoplasma sp. Positive (n) | Mycoplasma sp. Negative (n) | |||
---|---|---|---|---|
Districts | Ahırlı (n: 3) | 0 | 3 | p = 0.322 χ2 = 25.112 |
Akören (n: 2) | 0 | 2 | ||
Akşehir (n: 2) | 0 | 2 | ||
Altınekin (n: 2) | 1 | 1 | ||
Beyşehir (n: 6) | 0 | 6 | ||
Bozkır (n: 6) | 1 | 5 | ||
Cihanbeyli (n: 4) | 0 | 4 | ||
Çumra (n: 13) | 0 | 13 | ||
Doğanhisar (n: 4) | 0 | 4 | ||
Ereğli (n: 9) | 0 | 9 | ||
Güneysınır (n: 1) | 0 | 1 | ||
Hadim (n: 4) | 1 | 3 | ||
Halkapınar (n: 1) | 0 | 1 | ||
Ilgın (n: 13) | 4 | 9 | ||
Kadınhanı (n: 10) | 0 | 10 | ||
Karapınar (n: 4) | 0 | 4 | ||
Karatay (n: 64) | 7 | 57 | ||
Kulu (n: 2) | 0 | 2 | ||
Meram (n: 65) | 2 | 63 | ||
Sarayönü (n: 6) | 0 | 6 | ||
Selçuklu (n: 149) | 19 | 130 | ||
Seydişehir (n: 9) | 1 | 8 | ||
Taşkent (n: 5) | 0 | 5 | ||
Breed | American Bobtail (n: 1) | 0 | 1 | p = 0.302 χ2 = 20.289 |
Ankara (n: 12) | 1 | 11 | ||
Bombay (n: 2) | 0 | 2 | ||
British Longhair (n: 3) | 0 | 3 | ||
British Shorthair (n: 27) | 2 | 25 | ||
Crossbreed (n: 176) | 27 | 149 | ||
Exotic Shorthair (n: 2) | 0 | 2 | ||
Maine Coon (n: 1) | 0 | 1 | ||
Norwegian Forest Cat (n: 1) | 0 | 1 | ||
Persian (n: 6) | 0 | 6 | ||
Russian Blue (n: 2) | 1 | 1 | ||
Sarman (n: 6) | 0 | 6 | ||
Scottish Fold (n: 20) | 0 | 20 | ||
Scottish Shorthair (n: 3) | 0 | 3 | ||
Scottish Straight (n: 1) | 0 | 1 | ||
Siamese (n: 1) | 0 | 1 | ||
Smokin (n: 1) | 0 | 1 | ||
Somali (n: 1) | 0 | 1 | ||
Tuxedo (n: 1) | 0 | 1 | ||
Tabby (n: 111) | 5 | 106 | ||
Van (n: 7) | 0 | 7 | ||
Gender | Female (n: 190) | 16 | 174 | p = 0.601 χ2 = 0.403 |
Male (n: 194) | 20 | 174 | ||
Age | >1 year (n: 57) | 0 | 57 | p = 0.005 χ2 = 6.924 |
<1 year (n: 327) | 36 | 291 | ||
Status | Owned (n: 300) Stray (n: 84) | 19 17 | 281 67 | p = 0.000 χ2 = 14.934 |
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Ceylan, C.; Culha, M.H.; Sonmez, G.; Selcuk, M.A.; Ider, M.; Evci, A.; Yılmaz, S.; Sevinc, F.; Ceylan, O. Feline Hemotropic Mycoplasma Species of Apparently Healthy Domestic Cats in Konya Province of Türkiye. Vet. Sci. 2024, 11, 530. https://doi.org/10.3390/vetsci11110530
Ceylan C, Culha MH, Sonmez G, Selcuk MA, Ider M, Evci A, Yılmaz S, Sevinc F, Ceylan O. Feline Hemotropic Mycoplasma Species of Apparently Healthy Domestic Cats in Konya Province of Türkiye. Veterinary Sciences. 2024; 11(11):530. https://doi.org/10.3390/vetsci11110530
Chicago/Turabian StyleCeylan, Ceylan, Muhammed Hudai Culha, Gonca Sonmez, Muhammed Ahmed Selcuk, Merve Ider, Ayşe Evci, Sule Yılmaz, Ferda Sevinc, and Onur Ceylan. 2024. "Feline Hemotropic Mycoplasma Species of Apparently Healthy Domestic Cats in Konya Province of Türkiye" Veterinary Sciences 11, no. 11: 530. https://doi.org/10.3390/vetsci11110530
APA StyleCeylan, C., Culha, M. H., Sonmez, G., Selcuk, M. A., Ider, M., Evci, A., Yılmaz, S., Sevinc, F., & Ceylan, O. (2024). Feline Hemotropic Mycoplasma Species of Apparently Healthy Domestic Cats in Konya Province of Türkiye. Veterinary Sciences, 11(11), 530. https://doi.org/10.3390/vetsci11110530