Assessment of the Macrophage Scavenger Receptor CD163 in Mediating Glaesserella parasuis Infection of Host Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Vector Construction, Transient Transfection and Stable Overexpression
2.3. Immunofluorescence
2.4. Bacterial Strain Culture
2.5. Adhesion Assays
2.6. Adhesion Study by Scanning Electron Microscope (SEM)
2.7. Real-Time Quantitative PCR (Q-PCR)
2.8. Solid-Phase Adhesion Assay
2.9. Agglutination Assay
2.10. Statistical Analysis
3. Results
3.1. Overexpression of Porcine CD163 in CHO-K1 Cells
3.2. The Role of CD163 in Mediating the Adhesion of G. parasuis to CHO-K1 Cells
3.3. Effect of CD163 on G. parasuis Adhesion to 3D4/21 Cells
3.4. Bindings of G. parasuis to the Extracellular SRCR Domains of CD163
3.5. Recognition of G. parasuis by CD163 in Triggering Inflammatory Factors
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Deng, X.; Li, S.; Zhu, Y.; Yu, B.; Zhang, J.; Fang, Q.; Li, Z.; Chen, H.; Zhou, H. Assessment of the Macrophage Scavenger Receptor CD163 in Mediating Glaesserella parasuis Infection of Host Cells. Vet. Sci. 2023, 10, 235. https://doi.org/10.3390/vetsci10030235
Deng X, Li S, Zhu Y, Yu B, Zhang J, Fang Q, Li Z, Chen H, Zhou H. Assessment of the Macrophage Scavenger Receptor CD163 in Mediating Glaesserella parasuis Infection of Host Cells. Veterinary Sciences. 2023; 10(3):235. https://doi.org/10.3390/vetsci10030235
Chicago/Turabian StyleDeng, Xiangwei, Shuilian Li, Ying Zhu, Bo Yu, Jing Zhang, Qianhai Fang, Zhimin Li, Hongbo Chen, and Huanhuan Zhou. 2023. "Assessment of the Macrophage Scavenger Receptor CD163 in Mediating Glaesserella parasuis Infection of Host Cells" Veterinary Sciences 10, no. 3: 235. https://doi.org/10.3390/vetsci10030235