Comparison of Adhesive Tape Impression Cytology, Hair Plucks, and Fungal Culture for the Diagnosis of Dermatophytosis in Dogs and Cats
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Population and Diagnostic Tests
2.2. Statistical Analysis
3. Results
3.1. Dogs
3.2. Cats
3.3. Statistical Analysis Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Data | Percentage |
---|---|
Breed | |
Mixed breed | 12/30 (40%) |
Boxer | 3/30 (10%) |
Pitbull | 3/30 (10%) |
Yorkshire terrier | 2/30 (6.7%) |
Others | 10/30 (33.3%) |
Gender | |
Male | 15/30 (50%) |
Female | 15/30 (50%) |
Age (median:2, range:1.5–14 y) | |
≤1 year | 13/30 (43.3%) |
1–2 Years | 3/30 (10%) |
>2 years | 14/30 (46.7%) |
Case | Clinical Form | Hair Plucks | Adhesive Tape Impression Cytology | Fungal Culture (DTM) |
---|---|---|---|---|
1 | A | Positive | Positive | M. canis |
2 | A | Positive | Negative | Negative |
3 | A | Positive | Positive | Negative |
4 | A | Positive | Positive | M. canis |
5 | A | Negative | Positive | Negative |
6 | A | Positive | Negative | M. canis |
7 | A | Positive | Positive | M. canis |
8 | A | Negative | Positive | M. canis |
9 | A | Negative | Positive | M. canis T. mentagrophytes |
10 | A | Positive | Negative | M. canis |
11 | A | Positive | Negative | Negative |
12 | A | Positive | Negative | M. canis |
13 | A | Positive | Positive | M. canis |
14 | A | Positive | Positive | Negative |
15 | A | Positive | Positive | Negative |
16 | A | Positive | Positive | M. canis |
17 | A | Negative | Positive | M. canis |
18 | A | Negative | Negative | M. canis |
19 | A | Positive | Positive | M. canis |
20 | K | Positive | Negative | M. canis |
21 | K | Positive | Positive | M. canis |
22 | K | Negative | Positive | M. canis |
23 | K | Negative | Positive | M. canis |
24 | K | Negative | Positive | M. canis |
25 | K | Negative | Positive | M. canis |
26 | K | Negative | Positive | M. canis |
27 | K | Positive | Positive | M. canis |
28 | K | Negative | Positive | Negative |
29 | K | Negative | Positive | M. canis |
30 | K | Positive | Positive | M. canis |
Diagnostic Test | Dogs (n = 30) | Dogs with Alopecia (n = 19) | Dogs with Kerion (n = 11) | Cats (n = 15) | Dogs and Cats (n = 45) | Dogs and Cats with Alopecia (n = 34) |
---|---|---|---|---|---|---|
Hair plucks | 18/30, 60.0% (40.6, 77.3) | 14/19, 73.7% (48.8, 90.9) | 4/11, 36.4% (10.9, 69.2) | 12/15, 80.0% (51.9, 95.7) | 30/45, 66.7% (51.1, 80.0) | 26/34, 76.5% (58.8, 89.3) |
Adhesive tape impression cytology | 23/30, 76.7% (57.7, 90.1) | 13/19, 68.4% (43.5, 87.4) | 10/11, 90.9% (58.7, 99.8) | 14/15, 93.3% (68.1, 99.8) | 37/45, 82.2% (67.9, 92.0) | 27/34, 79.4% (62.1, 91.3) |
Fungal culture (DTM) | 23/30, 76.7% (57.7, 90.1) | 13/19, 68.4% (43.5, 87.4) | 10/11, 90.9% (58.7, 99.8) | 13/15, 86.7% (59.5, 98.3) | 36/45, 80.0% (65.4, 90.4) | 26/34, 76.5% (58.8, 89.3) |
Data | Percentage |
---|---|
Breed | |
DSH | 14/15 (93.3%) |
Persian | 1/15 (6.7%) |
Gender | |
Male | 9/15 (60%) |
Female | 6/15 (40%) |
Age (median: 2.5 months, range: 1.5 month–11 y) | |
≤6 months | 13/15 (86.7%) |
>6 months | 2/15 (13.3%) |
Case | Hair Plucks | Adhesive Tape Impression Cytology | Fungal Culture (DTM) |
---|---|---|---|
1 | Negative | Positive | M. canis |
2 | Positive | Positive | M. canis |
3 | Positive | Positive | Negative |
4 | Positive | Positive | Negative |
5 | Positive | Positive | M. canis |
6 | Negative | Negative | M. canis |
7 | Positive | Positive | M. canis |
8 | Positive | Positive | M. canis |
9 | Positive | Positive | T. mentagrophytes |
10 | Positive | Positive | M. canis |
11 | Positive | Positive | M. canis |
12 | Positive | Positive | M. canis |
13 | Positive | Positive | M. canis |
14 | Negative | Positive | M. canis |
15 | Positive | Positive | M. canis |
Comparison of Diagnostic Tests | Measures of Agreement | Cats (n = 15) | Dogs (n = 30) | Cats and Dogs (n = 45) | Cats and Dogs with Alopecia (n = 34) | Dogs with Alopecia (n = 19) | Dogs with Kerion (n = 11) |
---|---|---|---|---|---|---|---|
ATI cytology vs. Hair plucks | % Agreement | 86.67 | 43.33 | 57.78 | 67.65 | 52.63 | 23.08 |
Cohen’s k | 0.444 | −0.269 | −0.075 | 0.06 | −0.148 | NA | |
P-McNemar Test | 0.475 | 0.332 | 0.169 | 1 | 1 | 0.078 | |
ATI cytology vs. fungal culture (DTM) | % Agreement | 80 | 66.67 | 71.11 | 67.65 | 52.38 | 81.82 |
Cohen’s k | NA | 0.068 | 0.058 | 0.06 | −0.061 | NA | |
P-McNemar Test | 1 | 0.752 | 1 | 1 | 0.724 | 0.48 | |
Hair plucks vs. Fungal culture (DTM) | % Agreement | 66.67 | 50 | 55.56 | 58.82 | 52.63 | 45.45 |
Cohen’s k | NA | −0.119 | −0.111 | −0.144 | −0.148 | 0.108 | |
P-McNemar Test | 1 | 0.302 | 0.359 | 0.789 | 1 | 0.041 * |
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Bouza-Rapti, P.; Karafylia, A.; Tamvakis, A.; Farmaki, R. Comparison of Adhesive Tape Impression Cytology, Hair Plucks, and Fungal Culture for the Diagnosis of Dermatophytosis in Dogs and Cats. Vet. Sci. 2023, 10, 183. https://doi.org/10.3390/vetsci10030183
Bouza-Rapti P, Karafylia A, Tamvakis A, Farmaki R. Comparison of Adhesive Tape Impression Cytology, Hair Plucks, and Fungal Culture for the Diagnosis of Dermatophytosis in Dogs and Cats. Veterinary Sciences. 2023; 10(3):183. https://doi.org/10.3390/vetsci10030183
Chicago/Turabian StyleBouza-Rapti, Pavlina, Anastasia Karafylia, Androniki Tamvakis, and Rania Farmaki. 2023. "Comparison of Adhesive Tape Impression Cytology, Hair Plucks, and Fungal Culture for the Diagnosis of Dermatophytosis in Dogs and Cats" Veterinary Sciences 10, no. 3: 183. https://doi.org/10.3390/vetsci10030183