Generation of Transgenic Sperm Expressing GFP by Lentivirus Transduction of Spermatogonial Stem Cells In Vivo in Cynomolgus Monkeys
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Ethics
2.2. EGFP Lentivirus
2.3. Injection of EGFP Lentivirus into the Rete of Cynomolgus Macaque Testicles
2.4. Pathology of Testicular Tissue
2.5. Immunofluorescence Examination
2.6. Semen Collection, Evaluation and Cryopreservation
2.7. Sperm DNA Isolation, q-PCR and Sequencing
2.8. Statistical Analysis
2.9. Data Availability
3. Results
3.1. Validation of Rete Testis Injection Method and Introduction of EGFP Lentiviruses
3.2. The Injection of EGFP Lentivirus Did Not Affect Spermatogenesis
3.3. EGFP Expressed in SSCs
3.4. Confirmation of the Generation of Transgenic Sperm and Sperm Cryopreservation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Wang, S.; Duan, Y.; Chen, B.; Qiu, S.; Huang, T.; Si, W. Generation of Transgenic Sperm Expressing GFP by Lentivirus Transduction of Spermatogonial Stem Cells In Vivo in Cynomolgus Monkeys. Vet. Sci. 2023, 10, 104. https://doi.org/10.3390/vetsci10020104
Wang S, Duan Y, Chen B, Qiu S, Huang T, Si W. Generation of Transgenic Sperm Expressing GFP by Lentivirus Transduction of Spermatogonial Stem Cells In Vivo in Cynomolgus Monkeys. Veterinary Sciences. 2023; 10(2):104. https://doi.org/10.3390/vetsci10020104
Chicago/Turabian StyleWang, Shengnan, Yanchao Duan, Bingbing Chen, Shuai Qiu, Tianzhuang Huang, and Wei Si. 2023. "Generation of Transgenic Sperm Expressing GFP by Lentivirus Transduction of Spermatogonial Stem Cells In Vivo in Cynomolgus Monkeys" Veterinary Sciences 10, no. 2: 104. https://doi.org/10.3390/vetsci10020104