Evaluation of a Manual Cytocentrifuge versus the Standard Automated Cytocentrifuge in the Analysis of Canine Cerebrospinal Fluid: A Case Series of 55 Dogs
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection and Case Selection
2.2. Cell Counts
2.3. Cytocentrifugation
2.4. Microscopic Evaluation
2.5. Pleocytosis Classification
2.6. Presence of Artifacts
2.7. Statistical Analysis
3. Results
3.1. Study Case Series
3.2. Cytocentrifugation Technique
3.3. Comparison of Cell Counts and WBCs Differential
3.4. Presence of Artifacts
3.5. Presence of RBCs
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Prospective Cases | Retrospective Cases | TOTAL | |
---|---|---|---|
No pleocytosis | 33 | 5 | 38 |
Pleocytosis | 15 | 2 | 17 |
Mild | 6 | 2 | 8 |
Moderate | 5 | 0 | 5 |
Severe | 4 | 0 | 4 |
TOTAL | 48 | 7 | 55 |
Pleocytosis | Automatic | Manual |
---|---|---|
Mild | ||
Case n. 08 | Mixed-cell | Mixed-cell, predominantly lymphocytic |
Case n. 10 | Monocytoid | Monocytoid |
Case n. 12 | Mixed-cell, predominantly lymphocytic | Mixed-cell, predominantly lymphocytic |
Case n. 13 | Mixed-cell, predominantly neutrophilic | Mixed-cell, predominantly neutrophilic |
Case n. 21 | Neutrophilic | Neutrophilic |
Case n. 27 | Lymphocytic | Lymphocytic |
Case n. 30 | Mixed-cell | Mixed cell |
Case n. 44 | Lymphocytic | Lymphocytic |
Moderate | ||
Case n. 05 | Neutrophilic | Neutrophilic |
Case n. 15 | Lymphocytic | Lymphocytic |
Case n. 32 | Mixed-cell, predominantly monocytoid | Mixed-cell |
Case n. 41 | Mixed-cell, predominantly lymphocytic | Mixed-cell, predominantly lymphocytic |
Case n. 55 | Lymphocytic | Lymphocytic |
Severe | ||
Case n. 03 | Lymphocytic | Lymphocytic |
Case n. 24 | Neutrophilic | Neutrophilic |
Case n. 47 | Lymphocytic | Lymphocytic |
Case n. 54 | Mixed-cell, predominantly monocytoid | Mixed-cell, predominantly monocytoid |
MANUAL | AUTOMATIC | |||
Presence | Total | |||
Presence | 31 | Presence | 36 | |
Absence | 10 | Absence | 11 | |
Total | 41 | Total | 47 |
MANUAL | AUTOMATIC | |||
Presence | Absence | Total | ||
Presence | 17 | 4 | 21 | |
Absence | 6 | 28 | 34 | |
Total | 23 | 32 | 55 |
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Fonte-Oliveira, L.; Pereira, A.; Gregório, H.; Ribeiro, J.; Correia-Gomes, C.; Marcos, R.; Santos, M. Evaluation of a Manual Cytocentrifuge versus the Standard Automated Cytocentrifuge in the Analysis of Canine Cerebrospinal Fluid: A Case Series of 55 Dogs. Vet. Sci. 2023, 10, 631. https://doi.org/10.3390/vetsci10110631
Fonte-Oliveira L, Pereira A, Gregório H, Ribeiro J, Correia-Gomes C, Marcos R, Santos M. Evaluation of a Manual Cytocentrifuge versus the Standard Automated Cytocentrifuge in the Analysis of Canine Cerebrospinal Fluid: A Case Series of 55 Dogs. Veterinary Sciences. 2023; 10(11):631. https://doi.org/10.3390/vetsci10110631
Chicago/Turabian StyleFonte-Oliveira, Luísa, André Pereira, Hugo Gregório, João Ribeiro, Carla Correia-Gomes, Ricardo Marcos, and Marta Santos. 2023. "Evaluation of a Manual Cytocentrifuge versus the Standard Automated Cytocentrifuge in the Analysis of Canine Cerebrospinal Fluid: A Case Series of 55 Dogs" Veterinary Sciences 10, no. 11: 631. https://doi.org/10.3390/vetsci10110631