Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Canine Sera and Ethical Considerations
2.2. Protein Expression and Purification
2.3. Diagnostic Tests and ELISA
2.4. Data Collection
2.5. Statistical Analyses
3. Results
3.1. Environmental and Clinical Characteristics of the Study Population
3.2. Large-Scale Evaluation of the Recombinant Q5 for the CVL Diagnosis
3.3. Comparison between EIE-LVC and Q5
3.4. Comparative Evaluation of Different Q5-Based ELISA Assays
3.5. Evaluation of the Lci13 Recombinant Protein
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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N = 406 | Characteristics | N (%) |
---|---|---|
Origin of the animal | Domiciled | 244 (60.1) |
Semi-domiciled | 4 (1.0) | |
Communitarian | 63 (15.5) | |
Errant | 6 (1.5) | |
NI | 89 (21.9) | |
Sex | Female | 176 (43.3) |
Male | 214 (52.7) | |
NI | 16 (3.9) | |
Age | Up to 12 months | 21 (5.2) |
One year old or older | 274 (67.5) | |
NI | 111 (27.3) | |
Human contact | Yes | 280 (69.0) |
No | 2 (0.5) | |
NI | 124 (30.5) | |
Type of shelter * | Intra-residence | 100 (24.6) |
Extra-residence | 51 (12.6) | |
Peri-residence | 70 (17.2) | |
NI | 185 (45.6) | |
Symptoms | Asymptomatic | 117 (28.8) |
Symptomatic | 48 (11.8) | |
NI | 241 (59.4) | |
Clinical characteristics | Generalized dermatitis | 35 (72.91) |
Onychogryphosis | 23 (47.91) | |
Weight loss | 22 (45.83) | |
Hair loss | 13 (27.08) | |
Keratoconjunctivitis | 7 (14.58) |
ELISA | Sensitivity % (n = 406) | Symptomatic (n = 48) | Asymptomatic (n = 117) | Specificity % (n = 48) | Accuracy % | Kappa |
---|---|---|---|---|---|---|
Q5-8M | 68 (63–72) | 92 (80–98) | 62 (53–71) | 94 (83–99) | 71 (66–75) | 0.48 (0.38–0.60) |
EIE-LVC | 67 (61–72) | 92 (80–98) | 58 (50–68) | Not assayed | Not assayed | 0.48 (0.40–0.60) |
Q5 (8 M) Positive | Q5 (8 M) Negative | Q5 (8 M) Positive | Q5 (8 M) Negative | |
---|---|---|---|---|
Q5 (2 M) Positive | Q5 (2 M) Positive | Q5 (2 M) Negative | Q5 (2 M) Negative | |
EIE-LVC positive | 4 | 7 | 4 | 21 |
EIE-LVC negative | 19 | 13 | 13 | 88 |
Total | 23 | 20 | 17 | 109 |
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de Araújo Paz, L.F.; da Silva, A.; da Silva, H.R.F.; Cavalcanti, M.P.; de Lima, V.M.F.; da Cunha Beltrão, M.R.O.; Silva, M.B.A.; de Melo Neto, O.P.; Medeiros, Z.M.; Santos, W.J.T.d. Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test. Vet. Sci. 2023, 10, 608. https://doi.org/10.3390/vetsci10100608
de Araújo Paz LF, da Silva A, da Silva HRF, Cavalcanti MP, de Lima VMF, da Cunha Beltrão MRO, Silva MBA, de Melo Neto OP, Medeiros ZM, Santos WJTd. Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test. Veterinary Sciences. 2023; 10(10):608. https://doi.org/10.3390/vetsci10100608
Chicago/Turabian Stylede Araújo Paz, Larissa Ferreira, Adalúcia da Silva, Hemilly Rayanne Ferreira da Silva, Milena Paiva Cavalcanti, Valeria Marçal Felix de Lima, Maria Rosário Oliveira da Cunha Beltrão, Maria Beatriz Araújo Silva, Osvaldo Pompílio de Melo Neto, Zulma Maria Medeiros, and Wagner José Tenório dos Santos. 2023. "Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test" Veterinary Sciences 10, no. 10: 608. https://doi.org/10.3390/vetsci10100608
APA Stylede Araújo Paz, L. F., da Silva, A., da Silva, H. R. F., Cavalcanti, M. P., de Lima, V. M. F., da Cunha Beltrão, M. R. O., Silva, M. B. A., de Melo Neto, O. P., Medeiros, Z. M., & Santos, W. J. T. d. (2023). Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test. Veterinary Sciences, 10(10), 608. https://doi.org/10.3390/vetsci10100608