First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. PCR Assay
2.3. Sequencing
2.4. Phylogenetic Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Crt.No | County | Hunting Ground | Total Samples | Positive Samples (%) | Negative Samples (%) |
---|---|---|---|---|---|
1. | Timiș | Bethausen | 9 | 2 (22.22%) | 7 (77.78%) |
Valea Lungă | 4 | - | 4 | ||
Făget | 1 | - | 1 | ||
Ohaba | 7 | 1 (14.28%) | 6 (85.72%) | ||
Surduc | 4 | - | 4 | ||
Traian Vuia | 5 | - | 5 | ||
Nevrincea | 3 | - | 3 | ||
2. | Arad | Săvârșin | 5 | 1 (20%) | 4 (80%) |
Dumbrava | 3 | - | 3 | ||
Moneasa | 1 | - | 1 | ||
Lipova | 3 | - | 3 | ||
Petriș | 2 | - | 2 | ||
Șimand | 4 | - | 4 | ||
Fiac | 1 | - | 1 | ||
TOTAL | 52 | 4 | 48 |
Sample No. | Sample Identification | Codon | Similar Sequence | Species | |
---|---|---|---|---|---|
Start | End | ||||
1. | 254 | 10 | 1310 | KJ995531.1 | Paramphistomum leydeni |
KJ995529.1 | |||||
AB973398.1 | |||||
HM209064.1 | |||||
2. | 255 | 10 | 1040 | KP201674.1 | Calicophoron daubneyi |
AY790883.1 | |||||
3. | 256 | 10 | 920 | AB973394.1 | Calicophoron daubneyi |
4. | 259 | 10 | 850 | KT182100.1 | Calicophoron daubneyi |
KT182096.1 |
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Morariu, S.; Sîrbu, C.B.; Tóth, A.G.; Dărăbuș, G.; Oprescu, I.; Mederle, N.; Ilie, M.S.; Imre, M.; Sîrbu, B.A.-M.; Solymosi, N.; et al. First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania. Vet. Sci. 2023, 10, 603. https://doi.org/10.3390/vetsci10100603
Morariu S, Sîrbu CB, Tóth AG, Dărăbuș G, Oprescu I, Mederle N, Ilie MS, Imre M, Sîrbu BA-M, Solymosi N, et al. First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania. Veterinary Sciences. 2023; 10(10):603. https://doi.org/10.3390/vetsci10100603
Chicago/Turabian StyleMorariu, Sorin, Cătălin Bogdan Sîrbu, Adrienn Gréta Tóth, Gheorghe Dărăbuș, Ion Oprescu, Narcisa Mederle, Marius Stelian Ilie, Mirela Imre, Beatrice Ana-Maria Sîrbu, Norbert Solymosi, and et al. 2023. "First Molecular Identification of Calicophoron daubneyi (Dinnik, 1962) and Paramphistomum leydeni (Nasmark, 1937) in Wild Ruminants from Romania" Veterinary Sciences 10, no. 10: 603. https://doi.org/10.3390/vetsci10100603