Epidemiology and Genetic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in the Hunan and Hebei Provinces of China
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Specimen Collection
2.2. Enzyme-Linked Immunosorbent Assay (ELISA)
2.3. Sample Handling and PRRSV Detection
2.4. Sequencing and Genetic Analysis
2.5. Data Analysis
3. Results
3.1. PRRSV Seroprevalence in Pig Herds in Hunan and Hebei Provinces
3.2. Molecular Detection of PRRSV in Clinical Samples
3.3. Phylogenetic Analysis of ORF5 Gene
3.4. Sequence Analysis of ORF5 Gene
3.5. Bioinformatic Analysis of the Complete Genome Sequences of PRRSV Strains
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Regions | Development Stages | Tested Samples | Positive Samples | Positive Rate (%) | 95%CI | p-Value |
---|---|---|---|---|---|---|
Hunan | Gilts | 534 | 413 | 77.34 | 73.79–80.89 | <0.01 |
Sows | 591 | 477 | 80.71 | 77.53–83.89 | <0.01 | |
Fattening pigs | 436 | 206 | 47.25 | 42.56–51.94 | Reference | |
Nursing pigs | 511 | 314 | 61.45 | 57.23–65.67 | <0.01 | |
Subtotal | 2072 | 1410 | 68.05 | 65.34–70.76 | - | |
Hebei | Gilts | 421 | 369 | 87.64 | 84.28–91.00 | <0.01 |
Sows | 655 | 570 | 87.02 | 84.26–89.78 | <0.01 | |
Fattening pigs | 507 | 291 | 57.40 | 53.09–61.70 | Reference | |
Nursing pigs | 352 | 290 | 82.39 | 78.41–86.37 | <0.01 | |
Subtotal | 1935 | 1520 | 78.55 | 76.72–80.37 | - | |
Total | 4007 | 2930 | 73.12 | 71.74–74.49 | - |
Region | Clinical Samples | Samples | Positive Samples | Positive Rate (%) | 95%CI | p-Value |
---|---|---|---|---|---|---|
Hunan | Healthy pigs in slaughterhouses | 2818 | 217 | 7.70 | 7.54–7.86 | <0.01 |
Healthy pigs in farms | 2072 | 47 | 2.70 | 2.00–3.40 | Reference | |
Diseased pigs in non-hazardous disposal sites | 359 | 65 | 18.11 | 14.13–22.09 | <0.01 | |
Hebei | Diseased pigs with clinical symptoms of PRRS | 550 | 153 | 27.82 | 24.07–31.56 | <0.01 |
Total | 5799 | 482 | 8.31 | 7.60–9.02 | - |
Identified Strain | Reference Strain | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Sublineage 8.1 | Sublineage 8.7 | Lineage 3 | Sublineage 1.8 | Sublineage 1.5 | VR-2332 | CH-1a | JXA1 | NADC30 | NADC34 | ||
Sublineage 8.1 (n = 3) | nt | 100.0 | 90.2 | 97.2 | 93.5 | 84.3 | 86.7 | ||||
aa | 100.0 | 92.0 | 96.5 | 91.0 | 83.1 | 86.5 | |||||
Sublineage 8.7 (n = 12) | nt | 91.5–94.2 | 96.7–99.8 | 87.2–89.2 | 92.7–95.2 | 97.0–99.5 | 81.5–87.0 | 86.6–87.7 | |||
aa | 89.0–91.5 | 89.5–98.0 | 86.1–88.1 | 89.6–93.0 | 96.5–99.5 | 82.1–84.6 | 86.1–87.1 | ||||
Lineage 3 (n = 4) | nt | 83.0–84.8 | 82.4–84.7 | 87.6–91.4 | 81.6–85.9 | 83.9–85.9 | 82.9–84.6 | 79.3–83.5 | 81.6–85.2 | ||
aa | 79.5–82.5 | 78.6–83.6 | 88.1–92.5 | 81.1–84.6 | 81.5–86.6 | 80.6–84.1 | 79.6–84.1 | 81.6–85.6 | |||
Sublineage 1.8 (n = 22) | nt | 83.2–87.2 | 81.4–87.1 | 79.6–84.9 | 89.2–99.5 | 83.3–87.1 | 84.1–88.4 | 83.3–87.1 | 90.5–94.2 | 86.1–92.5 | |
aa | 81.5–85.5 | 80.6–86.1 | 77.6–87.1 | 88.1–99.5 | 81.6–86.6 | 82.1–87.1 | 82.6–87.6 | 91.5–94.5 | 86.1–92.5 | ||
Sublineage 1.5 (n = 2) | nt | 85.1–85.4 | 86.2–87.1 | 80.8–84.4 | 86.1–87.9 | 99.7 | 81.1–87.7 | 86.6–86.9 | 86.6–86.9 | 84.8–85.5 | 95.9–96.2 |
aa | 84.0–85.0 | 84.6–87.1 | 79.0–84.1 | 84.6–89.6 | 99.0 | 85.1–86.1 | 84.6–85.6 | 85.6–86.6 | 87.1–88.1 | 93.5–94.5 |
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Zhai, W.; Yu, S.; Zhang, P.; Lin, Y.; Ge, S.; Zhang, T.; Zhang, K.; He, S.; Hu, Q.; Tang, X.; et al. Epidemiology and Genetic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in the Hunan and Hebei Provinces of China. Vet. Sci. 2023, 10, 63. https://doi.org/10.3390/vetsci10010063
Zhai W, Yu S, Zhang P, Lin Y, Ge S, Zhang T, Zhang K, He S, Hu Q, Tang X, et al. Epidemiology and Genetic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in the Hunan and Hebei Provinces of China. Veterinary Sciences. 2023; 10(1):63. https://doi.org/10.3390/vetsci10010063
Chicago/Turabian StyleZhai, Wang, Siyu Yu, Pengxuan Zhang, Yuan Lin, Shenghu Ge, Taojie Zhang, Kun Zhang, Shicheng He, Qiaoyun Hu, Xiaomin Tang, and et al. 2023. "Epidemiology and Genetic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in the Hunan and Hebei Provinces of China" Veterinary Sciences 10, no. 1: 63. https://doi.org/10.3390/vetsci10010063