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Article

Viral Targets in the Human Interactome with Comprehensive Centrality Analysis: SARS-CoV-2, a Case Study

by
Nilesh Kumar
1,2 and
M. Shahid Mukhtar
1,3,*
1
Department of Biology, University of Alabama at Birmingham, 3100 East Science Hall, 902 14th Street South, Birmingham, AL 35294, USA
2
IRCP—Biological Data Sciences, University of Alabama at Birmingham, Birmingham, AL 35233, USA
3
Department of Genetics & Biochemistry, Clemson University, 105 Collings St. Biosystems Research Complex, Clemson, SC 29634, USA
*
Author to whom correspondence should be addressed.
Data 2024, 9(8), 101; https://doi.org/10.3390/data9080101
Submission received: 29 April 2024 / Revised: 5 August 2024 / Accepted: 17 August 2024 / Published: 20 August 2024

Abstract

Network centrality analyses have proven to be successful in identifying important nodes in diverse host–pathogen interactomes. The current study presents a comprehensive investigation of the human interactome and SARS-CoV-2 host targets. We first constructed a comprehensive human interactome by compiling experimentally validated protein–protein interactions (PPIs) from eight distinct sources. Additionally, we compiled a comprehensive list of 1449 SARS-CoV-2 host proteins and analyzed their interactions within the human interactome, which identified enriched biological processes and pathways. Seven diverse topological features were employed to reveal the enrichment of the SARS-CoV-2 targets in the human interactome, with closeness centrality emerging as the most effective metric. Furthermore, a novel approach called CentralityCosDist was employed to predict SARS-CoV-2 targets, which proved to be effective in expanding the pool of predicted targets. Pathway enrichment analyses further elucidated the functional roles and potential mechanisms associated with predicted targets. Overall, this study provides valuable insights into the complex interplay between SARS-CoV-2 and the host’s cellular machinery, contributing to a deeper understanding of viral infection and immune response modulation.

1. Introduction

Networks consist of nodes, which represent the system components within the network, and edges, which represent the connections or relationships between these entities [1,2,3]. In biological systems, especially in the contexts of molecular biology and systems biology, networks often focus on direct and indirect interactions among genes and their products. For instance, in protein–protein interaction (PPI) networks, every node symbolizes a protein, while edges denote the physical interactions occurring between them. Cataloging PPIs on a proteome-wide scale is commonly referred to as interactomes [1,2,3]. Interactomes exemplify the intricate web of static and dynamic interactions within living organisms, occurring both under normal steady-state conditions and in response to internal cues or external stressors. Such protein complexes play crucial functions in diverse signaling cascades, distinct cellular pathways, and a wide array of biological processes [4,5]. Specialized pathogens, including viruses, bacteria, and eukaryotic parasites, deploy a range of pathogenic molecules, including virulent proteins. These proteins have evolved to interact with crucial targets within the host’s interactomes, leading to significant rewiring of the information flow [2,3,6,7,8,9,10,11,12,13,14,15,16,17,18]. This manipulation of host interactomes plays a pivotal role in causing disease. Therefore, analyzing the network architecture and structural properties of host–pathogen interactomes may unveil novel components in microbial pathogenicity.
In cellular networks, this network pattern emerges from universal principles that govern network organization, indicating consistency across various network characteristics. For instance, scale-free topology describes a structure where connections among nodes exhibit a power law distribution, characterized by a few highly connected nodes and many with few connections. This “low number of nodes possessing increased connectivity” suggests that in these networks, a handful of nodes serves as hubs, connecting a significant portion of the network together. These hubs play a crucial role in maintaining efficient communication within the network, as they facilitate the flow of information between different regions or clusters of nodes. Besides network connectivity, node position is another crucial concept. Node betweenness centrality is a measure of how often a node serves as a bridge along the shortest paths between pairs of other nodes in the network. Nodes with high betweenness centralities (bottlenecks) are positioned strategically in the network, acting as critical players in a biological network. Indeed, hubs and bottlenecks are the key targets for various pathogens, including viruses, bacteria, and eukaryotic pathogens, across plant and animal interactomes. For instance, following the construction of a comprehensive human interactome, the analysis of the network centrality unveiled that pan-viral targets predominantly consist of hubs positioned at the network’s core and are enriched in fundamental biological processes [19]. A meta-analysis of host–virus interactions across 17 different viruses indicated that viruses tend to target bottlenecks and hubs. This demonstrates that viruses have evolved to disrupt the scale-free human interactome by targeting hubs and proteins that serve as crucial communication nodes [20]. Therefore, the connectivity and position of specific nodes potentially allow for us to understand how viral pathogens exploit vulnerable hosts’ cellular networks, facilitating infection, replication, or immune evasion. In addition, deciphering viral pathogenesis can better prepare us for future pandemics, similar to the recent global pandemic of COVID-19, caused by the highly contagious and pathogenic SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2).
In this study, we have constructed a comprehensive human interactome by integrating PPIs from eight large-scale experimental studies and databases. Additionally, we have compiled a comprehensive set of host targets of SARS-CoV-2. We conducted network topology analysis that involved computing seven diverse centrality measures, which were then applied to the human interactome. Subsequently, we overlaid those centrality-based prioritized nodes with SARS-CoV-2 targets. Our findings indicate that the load centrality is the most effective in predicting viral targets, followed by PageRank, hubs, and bottlenecks. We also explored how combining diverse topological features can enhance the predictive power. Furthermore, we utilized our recently developed algorithm, CentralityCosDist [21], to predict viral targets, even in the presence of a low fraction of known viral targets. Overall, this integrative systems biology framework can significantly contribute to our understanding of viral pathogenesis, the identification of crucial host biological pathways, and the discovery of potential therapeutic targets.

2. Materials and Methods

2.1. Construction of a Comprehensive Human Protein Interaction Network

In this study, we constructed a comprehensive human protein–protein interaction (PPI) network by integrating data from several large-scale experimental studies and public databases. Our network included PPIs from BioPlex (“BioPlex_HCT116_55K_Dec_2019” and “BioPlex_293T_10K_Dec_2019”) [18,22,23], CoFrac [24], CUBIC [25], HuRI (“HIR2_EXP”, “HIR2_PRED”, and “HI_union”) [26], and STRING v12 [27] (Figure 1B and Table S1). We also compiled a set of 1449 SARS-CoV-2 host proteins from three recent studies [28,29,30].
To ensure consistency, we mapped all the protein identifiers to UniProt accession numbers using the UniProt API when necessary. Merging the PPI datasets resulted in a large network, with 26,028 nodes (proteins) and 825,682 edges (interactions) (Figure 1B and Table S1). Interestingly, 1445 of the 1449 SARS-CoV-2 host’s interactor proteins were present in this merged network (Figure 1E).

2.2. Building a SARS-CoV-2 Host-Protein-Specific Human Protein Interaction Network

From this comprehensive network, we extracted a sub-network we call the “open network” that focuses specifically on the 1445 SARS-CoV-2 host proteins and their 19,255 direct interactions. This sub-network contains 20,700 nodes and 260,231 edges (Figure 1E).
Our integrative approach allowed for us to construct a high-quality human PPI network that can serve as a valuable resource for studying viral pathogenesis and identifying potential therapeutic targets. By combining multiple large-scale datasets, we were able to expand the coverage of known human PPIs and pinpoint those most relevant to SARS-CoV-2 infection.

2.3. Network Centrality Analysis

In our investigation of the “open network”, we employed a range of seven distinct centrality measures to pinpoint the most crucial nodes within the network. These include degree centrality, betweenness centrality, eigenvector centrality, closeness centrality, load centrality, and PageRank; each offers a unique perspective on a node’s importance (Figure S1). By calculating each centrality measure for every node, we were able to generate a ranked list, with the most central nodes appearing at the top (Figure 2A). Interestingly, upon examining this ranked list, we discovered that several of the human proteins known to interact with SARS-CoV-2 were positioned prominently near the top. This finding suggests that these highly central proteins may play a vital role in the virus’s interaction with human cells.

2.4. Ranking Nodes with Respect to the SARS-CoV-2 Host Proteins

To identify novel human proteins that SARS-CoV-2 might potentially hijack, we employed a recently developed method called CentralityCosDist in the “open network” (Figure S3). This method goes beyond standard network analysis by incorporating multiple centrality measures. In essence, centrality measures evaluate a node’s importance within the network. In this study, we utilized seven such measures: degree centrality, betweenness centrality, eigenvector centrality, closeness centrality, clustering coefficient, load centrality, and PageRank. Furthermore, to enhance the effectiveness of the CentralityCosDist, we strategically selected “seed nodes”. These seed nodes act as reference points within the network, guiding the algorithm’s search for new potential viral targets. We implemented a two-pronged approach for seed node selection:
i.
SARS-CoV-2 Host-Protein-Based Seeds: We utilized two variations. In the first, we selected 10% of the 1445 known SARS-CoV-2 host proteins. In the second variation, we included all 1445 known host proteins. This approach prioritizes nodes with established connections to the virus;
ii.
Centrality-Based Seeds: We identified the top 10% of the nodes based on three centrality measures: degree centrality, betweenness centrality, and closeness centrality. These nodes are inherently very interconnected within the network, making them prime candidates for further investigation.
By combining these seed node strategies with CentralityCosDist, we aimed to cast a wide net and uncover a diverse range of potential SARS-CoV-2 targets within the human protein network.
To preprocess our protein–protein interaction (PPI) data and perform network analyses, we utilized the Python programming language (version 3.11.4) and the NetworkX package (version 3.1). NetworkX is a widely used, open-source Python library for creating, manipulating, and studying the structures and dynamics of complex networks. To visualize our networks and generate publication-quality figures, we used Cytoscape [31] software (version 3.10.2). To gain biological insights from our network analysis, we performed functional enrichment analysis using the Metascape web application [32].

3. Results and Discussion

3.1. A Comprehensive Human Protein–Protein Interactome and SARS-CoV-2 Host Targets

To comprehend the intricate network properties of the SARS-CoV-2 host targets, our initial step involved the assembly of a comprehensive human interactome. This encompassed the compilation of experimentally validated PPIs from eight distinct sources. Among these varied resources, the STRING database [33] emerged as being pivotal, containing a vast repository of 561,769 experimentally validated interactions involving approximately 18,000 proteins (Figure 1A,B and Table S1). Additionally, we curated PPI data from additional proteome-scale interactome studies, namely, Human Interactomes I and II, BioPlex, QUBIC, and CoFrac (as reviewed in [34]), alongside contributions from the Human Interactome Resource (HIR) [35] (Figure 1A,B and Table S1). Following the integration of these diverse PPI datasets, our resultant integrated human interactome featured 825,682 interactions and 26,000 nodes. Our previous study resulted in an experimentally validated high-quality interactome comprising 18,906 nodes and 444,633 edges [36]. In the current study, we have nearly doubled the number of interactions, creating a significantly expanded dataset. This enhanced interactome serves as a valuable resource for future unrelated studies, providing a more comprehensive understanding of network dynamics and facilitating further exploration into complex biological processes. Subsequently, we compiled an exhaustive compendium of SARS-CoV-2 host targets, which comprises 1449 host proteins (see the methods section for details; Table S1). Upon querying these host targets within the human interactome framework, we discovered the presence of 1445 host targets (Table S1). Intriguingly, among these, 20 host targets were found as singletons, while the remaining 1429 were found to interact amongst themselves, forming a “closed network” encompassing 31,000 interactions (Figure 1C, Table S1). Pathway enrichment analyses conducted using Metascape revealed a plethora of enriched biological processes. These included “intracellular protein transport”, “membrane organization”, “viral infection pathways”, “RNA metabolism”, “ER protein processing and Golgi vesicle trafficking”, “RhoGTPase signaling”, “influenza infection”, “cell cycle regulation”, and “neutrophil degradation”, among several other pathways (Figure 1D). Akin to this study, another proteome-scale mapping of SARS-CoV-2 targets identified 739 high-confidence binary and co-complex interactions. These interactions were found to be enriched in pathways including protein translation, mRNA splicing, Golgi transportation, neutrophil-mediated immunity, and glucose metabolism [29]. In contrast to another recent study that relied solely on the STRING database and focused on 1432 distinct proteins targeted by SARS-CoV-2 [37], our approach presents a significantly more comprehensive analysis. Their study constructed a SARS-CoV-2-relevant human interactome comprising 1111 nodes and 7043 edges, identifying enriched biological processes and functional categories, such as neutrophil-mediated immunity (GO:0002446), neutrophil-activation-involved immune responses (GO:0002283), and viral processes (GO:0016032), from the biological process category. Additionally, functional categories, including dolichyl-diphosphooligosaccharide–protein glycotransferase activity (GO:0004579), GDP binding (GO:0019003), cadherin binding (GO:0045296), ATPase activity (GO:0016887), and focal adhesion (GO:0005925), were highlighted [37]. By contrast, our methodology involved integrating data from diverse sources, resulting in a more extensive human interactome. This broader dataset facilitated a deeper exploration of network properties and pathway analyses, allowing for us to uncover a wider range of biological insights. These findings offer valuable insights into the complex interplay between SARS-CoV-2 and the host’s cellular machinery. By shedding light on the molecular pathways involved in viral infection and immune response modulation, our study contributes to a deeper understanding of the pathogenesis of SARS-CoV-2 infection and may inform the development of novel therapeutic strategies. To comprehensively understand SARS-CoV-2 targets and their interactions within a broader context, we extended our analysis to encompass the first neighbors of these interactions within the human interactome (Figure 1E). This resulted in the establishment of a comprehensive human–SARS-CoV-2 interactome, characterized by 20,700 nodes and 260,231 interactions (Figure 1E, Table S1). By doing so, we aimed to understand the connectivity and positioning of SARS-CoV-2 targets within the broader network, providing insights into their relationships and functional roles within the entire human interactome.

3.2. Diverse Centrality Measures to Reveal the Enrichment of SARS-CoV-2 Targets in the Human Interactome

Centrality measures are crucial in network analysis, evaluating nodes’ significance by examining their connections [21]. Leveraging centrality measures enriches our understanding of network behavior, facilitating targeted interventions and revealing underlying dynamics across various biological systems. In network topology, structural centralities can generally be classified into three groups [38,39,40]: (i) neighborhood-based centralities, such as degree centrality, coreness, and LocalRank, which assess the influences of nodes by considering their relationships with neighboring nodes; (ii) path-based centralities, including the shortest path length, betweenness centrality, information centrality, closeness centrality, and Katz centrality, which gauge nodes’ influences based on the distances between them within the network; and (iii) iterative refinement centralities, such as the eigenvector centrality, PageRank, and LeaderRank, which evaluate nodes’ influences by taking into account both the mutual interactions of node neighbors and their overall impact within the network [38,39]. We selected seven diverse centrality measures, representing the three distinct groups discussed above (Supplementary Figure S1), to discern the enrichment patterns of the SARS-CoV-2 host targets. We employed a top 10% threshold (2602 out of 26,028 total nodes) to categorize nodes with high centrality across the seven topology measures. The closeness centrality emerged as the most effective metric for identifying 694 SARS-CoV-2 host targets, exhibiting a prediction power exceeding 26%. Following closely behind was high-eigenvector information centrality nodes as well as hubs and bottlenecks, with prediction powers of 24.6%, 24.1%, and 23%, respectively (Figure 2A, Table S2). Although our network analysis methods provided a ranking of potential protein targets, it is important to note that these rankings were derived from and supported by underlying experimentally validated interactions. We acknowledge that experimental validation of all the predicted interactions is beyond the scope of this computational study. However, our approach leverages a wealth of existing experimental data to make informed predictions about potential SARS-CoV-2 targets. These predictions can serve as valuable hypotheses for future experimental studies focused on validating specific virus–host protein interactions.
Previous studies have made significant contributions to our understanding of SARS-CoV-2–host protein interactions [41,42]. A tissue-specific network analysis approach identified potential SARS-CoV-2 targets across different human tissues. This study highlighted the importance for considering tissue specificity in virus–host interactions and identified several key proteins and pathways involved in SARS-CoV-2 infection [42]. Using multiple experimental approaches, including affinity purification mass spectrometry and proximity labeling, a comprehensive map of the SARS-CoV-2–human ‘contactome’ was provided. This study identified both previously known and novel virus–host protein interactions, offering insights into the molecular mechanisms of SARS-CoV-2 infection [41]. Our study builds upon previous works by integrating a broader range of experimental datasets and employing different algorithms and prioritization methods, potentially leading to the identification of both overlapping and novel targets. Additionally, our analysis incorporates the latest-available data, capturing interactions that may not have been known or considered in earlier studies. We observed both overlaps and differences in the protein targets identified by our analysis compared to previous studies. The overlapping targets validate our approach and highlight the significance of these proteins in SARS-CoV-2 infection. Specifically, 168 out of the 170 HuSCI proteins identified by Kim et al., and all the organ-specific proteins identified by Feng et al., are present in our merged network, with a significant overlap of 1445 SARS-CoV-2 host interactor proteins from both studies (Figure S2). The differences in the identified targets can be attributed to dataset differences, methodological variations, and the inherent biological complexity of virus–host interactions.
In a previous study, four different centrality measures (degree, betweenness, closeness, and eigenvector) were employed on 332 SARS-CoV-2 host targets [43]. That study concluded that all the centrality measures play a significant role in identifying crucial nodes within a network. As a result, median ranking scores were calculated, and the top 20 candidates were selected for functional similarity analysis. This analysis informed the construction of a drug–protein interaction network [43]. Similarly, in our previous study, network centrality analyses utilizing seven different centrality metrics identified 28 high-value SARS-CoV-2 targets. These targets are likely involved in crucial processes, such as viral entry, proliferation, and survival, contributing to the establishment of the infection and the progression of the disease [36]. In the present study, we identified 406 nodes that are common across seven different centrality measures. Additionally, we found 66 and 113 nodes that are common across six and five centrality measures, respectively. This indicates a robust convergence of nodes across multiple centrality metrics, highlighting their potential significance in the network and potential therapeutic targets. Organelle assembly, protein localization, adaptive immune response, cytokine signaling, diseases of signal transduction, ribosome biogenesis, and neurodegeneration pathways emerge as prominent biological pathways enriched across centrality measures (Figure 3). Taken together, we discovered that centrality measures, particularly the closeness centrality, high-eigenvector centrality, and information centrality nodes, as well as hubs and bottlenecks, are effective metrics to identify SARS-CoV-2 host targets.

3.3. CentralityCosDist to Predict SARS-CoV-2 Targets

In our study, we addressed another question of how to expand the limited number of available SARS-CoV-2 targets, aiming to predict additional targets by leveraging network-based gene prioritization methods. The existing methods can generally be categorized into four groups: guilt by association, centrality measures, network propagation/random walk, and network clustering/communality analysis [21]. Although each method has its strengths, it also comes with its own limitations. To address these limitations, we introduced a novel approach called CentralityCosDist [21]. Briefly, this method starts with a set of seed nodes. In a given network, each node is represented as a seven-dimensional vector using seven diverse centrality metrices. The cosine distances between the seed nodes are computed using these centrality vectors. By averaging the distances between the seed nodes and each of the other nodes, all the nodes are ranked (Figure S3). This approach allows for prioritizing and ranking the nodes based on their similarity to the seed nodes in the seven-dimensional vector space (Figure S3). We applied CentralityCosDist to a randomly selected a set of 144 SARS-CoV-2 targets (with target seed 10 representing 10% of all the known host targets). This was compared with target seed 100, representing all the known host targets in our study. Remarkably, this led us to predict a total of 243 targets spanning 2070 nodes, with a prediction power exceeding 11.73% (Figure 4A). A similar number of targets were predicted if seed nodes were selected among the closeness centrality or hubs (Figure 4A). This highlights the effectiveness of our approach in expanding the pool of predicted targets when a limited number of host targets are known. This offers valuable insights for further research into SARS-CoV-2 biology and potential therapeutic interventions. Pathway enrichment analyses for “target seed 10” revealed enrichment in several biological pathways. These pathways include the protein catabolic process, protein localizations, vesicle-mediated transport, membrane organizations, protein processing, and VEGFAR2 signaling, among others (Figure 4B). This comprehensive analysis provides valuable insights into the functional roles and potential mechanisms associated with “target seed 10”, shedding light on its involvement in various cellular processes.

4. Conclusions

The current study led to the construction of a comprehensive dataset of the human protein–protein interactome and SARS-CoV-2 host targets. This expanded dataset serves as a valuable resource for future studies on other viruses and other biological questions. Additionally, we curated a comprehensive list of 1449 SARS-CoV-2 host proteins and analyzed their interactions within the human interactome. Moreover, we employed seven diverse centrality measures in identifying crucial nodes within the network, revealing the load centrality as being the most effective metric for predicting SARS-CoV-2 host targets. Our analysis highlighted the convergence of nodes across multiple centrality metrics, indicating their potential significance in the pathobiology of SARS-CoV-2. Additionally, we introduced a novel approach called CentralityCosDist to predict SARS-CoV-2 targets, which proved to be effective in expanding the pool of predicted targets. Pathway enrichment analyses further elucidated the functional roles and potential mechanisms associated with these predicted targets, providing valuable insights into their involvements in various cellular processes. Overall, our study offers a comprehensive understanding of SARS-CoV-2 host targets and their interactions within the human interactome. This may pave the way for future research into the pathogenesis of SARS-CoV-2 infection and the development of novel therapeutic strategies.

Supplementary Materials

The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/data9080101/s1: Figure S1: Centrality metrics in network analysis; Figure S2: Target overlaps with previous studies; Figure S3: Schematics of CentralityCosDist pipeline; Table S1: Human PPIs and seed sets; Table S2: Human PPIs (open) and seed sets; Table S3: CentralityCosDist analysis results.

Author Contributions

N.K. and M.S.M. originally devised and refined the protocol. N.K. performed the initial analysis and data curation. M.S.M. and N.K. conceived the project and wrote the manuscript. M.S.M. supervised the analysis. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by NSF award IOS-2038872 to M.S.M.

Institutional Review Board Statement

Not applicable for this study.

Informed Consent Statement

Not applicable for this study.

Data Availability Statement

The PPIs and SARS-CoV-2 host targets referenced in this study are available in the Supplementary tables. The custom code used in this study is available from the corresponding author upon reasonable request.

Acknowledgments

The authors gratefully acknowledge the resources provided by the University of Alabama at Birmingham’s IT-Research Computing group for high-performance computing (HPC) support and CPU time on the Cheaha compute cluster.

Conflicts of Interest

The authors declare no conflicts of interest.

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Figure 1. (A) Integration of multiple protein interaction networks to identify a consensus target cluster. This figure illustrates a funnel-like integration strategy, where distinct protein interaction networks from various databases are combined. Each colored cluster represents a unique network. These networks converge to identify a consensus ‘target’ set, denoted in red. (B) Details of the numbers of nodes and interactions for a particular network. BioPlex 55k, HuRI, STRING, HIR2 Ex, CUBIC, CoFrac, BioPlex 10k, and HIR2 P. (C) Schematic to display SARS-CoV-2 open-reading frames (ORFs) targeting the human interactome. (D) Analysis of the functional enrichment of CentralityCosDist prioritized genes using the Metascape web application. (E) Integrated or merged human PPI (blue) network and target proteins (red).
Figure 1. (A) Integration of multiple protein interaction networks to identify a consensus target cluster. This figure illustrates a funnel-like integration strategy, where distinct protein interaction networks from various databases are combined. Each colored cluster represents a unique network. These networks converge to identify a consensus ‘target’ set, denoted in red. (B) Details of the numbers of nodes and interactions for a particular network. BioPlex 55k, HuRI, STRING, HIR2 Ex, CUBIC, CoFrac, BioPlex 10k, and HIR2 P. (C) Schematic to display SARS-CoV-2 open-reading frames (ORFs) targeting the human interactome. (D) Analysis of the functional enrichment of CentralityCosDist prioritized genes using the Metascape web application. (E) Integrated or merged human PPI (blue) network and target proteins (red).
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Figure 2. (A) Centrality measure and target protein overlap in the human–SARS-CoV-2 interactome. This heatmap visualizes the overlap of the target protein nodes based on different centrality measures within an open network. The x-axis lists various centrality metrics, such as degree, betweenness, eigenvector, closeness, local, and PageRank centralities. The y-axis represents the percentage of the nodes within the network, ranging from the top 1% to 100%. Each cell contains a numerical value indicating the number of nodes that are target proteins across the different centrality measures, with the corresponding color gradient reflecting the degree of the overlap—ranging from low (blue) to high (yellow). The high degree of the overlap is indicated by the consistent appearance of higher values (indicated by yellower shades) at the top percentage, particularly in the top 10%. (B) UpSet plot of 10% open network’s target centrality overlap. Overlap of top 10% (centrality-ranked nodes) target proteins’ overlapping nodes.
Figure 2. (A) Centrality measure and target protein overlap in the human–SARS-CoV-2 interactome. This heatmap visualizes the overlap of the target protein nodes based on different centrality measures within an open network. The x-axis lists various centrality metrics, such as degree, betweenness, eigenvector, closeness, local, and PageRank centralities. The y-axis represents the percentage of the nodes within the network, ranging from the top 1% to 100%. Each cell contains a numerical value indicating the number of nodes that are target proteins across the different centrality measures, with the corresponding color gradient reflecting the degree of the overlap—ranging from low (blue) to high (yellow). The high degree of the overlap is indicated by the consistent appearance of higher values (indicated by yellower shades) at the top percentage, particularly in the top 10%. (B) UpSet plot of 10% open network’s target centrality overlap. Overlap of top 10% (centrality-ranked nodes) target proteins’ overlapping nodes.
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Figure 3. Pathway analyses for host targets for diverse centralities.
Figure 3. Pathway analyses for host targets for diverse centralities.
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Figure 4. (A) Comparative analysis of CentralityCosDist outputs with various seed sets. This figure presents an UpSet plot, illustrating the intersections of the top nodes identified by the CentralityCosDist tool across different sets of seed networks. The seeds for the CentralityCosDist analysis vary, including ‘open network betweenness’, ‘open network closeness’, and ‘open network degree’, along with ‘target seed 10’, which represent the top 10% of the seeds chosen at random, and ‘target seed 100’, denoting the full set of seeds, amounting to 1449 nodes. The horizontal bars across the top of the plot indicate the number of top nodes (2070) identified in each CentralityCosDist run, consistent across all the seed sets. The filled circles and connecting lines in the matrix reveal the overlapping results between different seed sets and the ‘target’, with red circles emphasizing where the ‘target’ overlaps with other sets. The vertical bars on the right side illustrate the intersection sizes. The largest intersection is observed with the full set of seeds (‘target seed 100’), while the intersections with ‘target seed 10’ and centrality-based seed sets show a graded distribution of smaller sizes. This analysis demonstrates the consistency of the CentralityCosDist tool in identifying the top nodes across various seed sets and particularly highlights the influences of different seed selection strategies on the outcome of the centrality distribution analysis. It offers a nuanced perspective on how the choice of seeds can affect the identification of the key targets within the network. (B) Pathway analyses for host targets that are identified through CentralityCosDist.
Figure 4. (A) Comparative analysis of CentralityCosDist outputs with various seed sets. This figure presents an UpSet plot, illustrating the intersections of the top nodes identified by the CentralityCosDist tool across different sets of seed networks. The seeds for the CentralityCosDist analysis vary, including ‘open network betweenness’, ‘open network closeness’, and ‘open network degree’, along with ‘target seed 10’, which represent the top 10% of the seeds chosen at random, and ‘target seed 100’, denoting the full set of seeds, amounting to 1449 nodes. The horizontal bars across the top of the plot indicate the number of top nodes (2070) identified in each CentralityCosDist run, consistent across all the seed sets. The filled circles and connecting lines in the matrix reveal the overlapping results between different seed sets and the ‘target’, with red circles emphasizing where the ‘target’ overlaps with other sets. The vertical bars on the right side illustrate the intersection sizes. The largest intersection is observed with the full set of seeds (‘target seed 100’), while the intersections with ‘target seed 10’ and centrality-based seed sets show a graded distribution of smaller sizes. This analysis demonstrates the consistency of the CentralityCosDist tool in identifying the top nodes across various seed sets and particularly highlights the influences of different seed selection strategies on the outcome of the centrality distribution analysis. It offers a nuanced perspective on how the choice of seeds can affect the identification of the key targets within the network. (B) Pathway analyses for host targets that are identified through CentralityCosDist.
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Kumar, N.; Mukhtar, M.S. Viral Targets in the Human Interactome with Comprehensive Centrality Analysis: SARS-CoV-2, a Case Study. Data 2024, 9, 101. https://doi.org/10.3390/data9080101

AMA Style

Kumar N, Mukhtar MS. Viral Targets in the Human Interactome with Comprehensive Centrality Analysis: SARS-CoV-2, a Case Study. Data. 2024; 9(8):101. https://doi.org/10.3390/data9080101

Chicago/Turabian Style

Kumar, Nilesh, and M. Shahid Mukhtar. 2024. "Viral Targets in the Human Interactome with Comprehensive Centrality Analysis: SARS-CoV-2, a Case Study" Data 9, no. 8: 101. https://doi.org/10.3390/data9080101

APA Style

Kumar, N., & Mukhtar, M. S. (2024). Viral Targets in the Human Interactome with Comprehensive Centrality Analysis: SARS-CoV-2, a Case Study. Data, 9(8), 101. https://doi.org/10.3390/data9080101

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