Population Genetics Data of 21 Autosomal STR Loci in the Romanian Population
Abstract
1. Introduction
2. Materials and Methods
- (a)
- DNA was extracted with QIAamp Investigator BioRobot Kit (Qiagen) on BioRobot Universal System (Qiagen) using the kit manufacturer’s protocol. The DNA extraction was followed by STR amplification with the Investigator 24plex QS kit (Qiagen) and a Veriti™ Thermal Cycler (ThermoFisher Scientific, Waltham, MA, USA) for 21 autosomal STR (A-STR) loci (Table 2), one Y chromosome STR (Y-STR) locus (DYS391), Amelogenin, and two quality controls, using the kit manufacturer’s protocol.
- (b)
- DNA was extracted with QIAsymphony DNA Investigator Kit (Qiagen) and QIAsymphony SP/AS instrument (Qiagen) using the kit manufacturer’s protocol. The DNA was quantified with Investigator Quantiplex Pro RGQ (Qiagen) and RotorGene Q Real-Time System (Qiagen) using the kit manufacturer’s protocol. PCR amplification was made with the Investigator 24plex QS kit (Qiagen) and a Veriti™ Thermal Cycler (ThermoFisher Scientific) for 21 A-STR loci (Table 2), one Y-STR locus (DYS391), Amelogenin, and two quality controls, using the kit manufacturer’s protocol.
- (c)
- DNA was extracted with Investigator STR GO! Lysis Buffer (Qiagen) using the kit manufacturer’s protocol. The DNA extraction was followed by STR amplification with the Investigator 24plex GO! Kit (Qiagen) and a Veriti™ Thermal Cycler (ThermoFisher Scientific) for 21 A-STR loci (Table 2), one Y-STR locus (DYS391), Amelogenin, and two quality controls, using the kit manufacturer’s protocol.
- (d)
- For some samples, no separate DNA extraction step was performed. Instead, the samples were subjected directly to PCR amplification using the Investigator 24plex GO! Kit (Qiagen) and a Veriti™ Thermal Cycler (ThermoFisher Scientific). This amplification targeted 21 A-STR loci (Table 2), one Y-STR locus (DYS391), the Amelogenin locus, and two quality control markers. The amplification was carried out following the manufacturer’s protocol.
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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County | Percent of the Romanian Population (According to 2021 Population Census) | Number of Analyzed Biological Samples |
---|---|---|
Alba | 1.71% | 7 * |
Arad | 2.15% | 22 |
Argeș | 2.99% | 30 |
Bacău | 3.16% | 32 |
Bihor | 2.89% | 29 |
Bistrița-Nasăud | 1.55% | 16 |
Botoșani | 2.06% | 21 |
Brașov | 2.87% | 29 |
Brăila | 1.48% | 15 |
Buzău | 2.13% | 21 |
Caraș-Severin | 1.29% | 13 |
Călărași | 1.49% | 5 * |
Cluj | 3.56% | 36 |
Constanța | 3.44% | 34 |
Covasna | 1.05% | 10 |
Dâmbovița | 2.52% | 25 |
Dolj | 3.15% | 30 * |
Galați | 2.61% | 26 |
Giurgiu | 1.38% | 8 * |
Gorj | 1.65% | 17 |
Harghita | 1.53% | 13 * |
Hunedoara | 1.90% | 19 |
Ialomița | 1.32% | 13 |
Iași | 3.99% | 40 |
Ilfov | 2.85% | 4 * |
Maramureș | 2.37% | 24 |
Mehedinți | 1.23% | 12 |
Mureș | 2.72% | 27 |
Neamț | 2.38% | 24 |
Olt | 2.01% | 20 |
Prahova | 3.65% | 36 |
Satu Mare | 1.74% | 4 * |
Sălaj | 1.11% | 11 |
Sibiu | 2.04% | 20 |
Suceava | 3.37% | 34 |
Teleorman | 1.70% | 17 |
Timiș | 3.41% | 28 * |
Tulcea | 1.01% | 10 |
Vaslui | 1.97% | 20 |
Vâlcea | 1.79% | 18 |
Vrancea | 1.76% | 18 |
Bucharest City | 9.01% | 90 |
Locus | Chromosomal Location |
---|---|
CSF1PO | 5q32 |
D1S1656 | 1q42.2 |
D2S441 | 2p14 |
D2S1338 | 2q35 |
D3S1358 | 3p21.31 |
D5S818 | 5q23.2 |
D7S820 | 7q21.11 |
D8S1179 | 8q24.13 |
D10S1248 | 10q26.3 |
D12S391 | 12p13.2 |
D13S317 | 13q31.1 |
D16S539 | 16q24.1 |
D18S51 | 18q21.33 |
D19S433 | 19q12 |
D21S11 | 21q21.1 |
D22S1045 | 22q12.3 |
FGA | 4q31.3 |
SE33 | 6q15 |
TH01 | 11p15.5 |
TPOX | 2p25.3 |
vWA | 12p13.31 |
Allele | CSF1PO | D1S1656 | D2S441 | D2S1338 | D3S1358 | D5S818 | D7S820 | D8S1179 | D10S1248 | D12S391 | D13S317 |
---|---|---|---|---|---|---|---|---|---|---|---|
7 | 0.003 | 0.023 | 0.001 | ||||||||
8 | 0.003 | 0.001 | 0.151 | 0.023 | 0.141 | ||||||
9 | 0.030 | 0.001 | 0.032 | 0.148 | 0.012 | 0.094 | |||||
10 | 0.269 | 0.001 | 0.170 | 0.079 | 0.249 | 0.074 | 0.001 | 0.061 | |||
11 | 0.315 | 0.106 | 0.321 | 0.001 | 0.310 | 0.244 | 0.072 | 0.002 | 0.309 | ||
11.3 | 0.086 | ||||||||||
12 | 0.316 | 0.126 | 0.037 | 0.001 | 0.378 | 0.156 | 0.121 | 0.021 | 0.270 | ||
12.3 | 0.001 | ||||||||||
13 | 0.057 | 0.102 | 0.033 | 0.002 | 0.184 | 0.025 | 0.307 | 0.228 | 0.098 | ||
13.3 | 0.001 | ||||||||||
14 | 0.008 | 0.074 | 0.303 | 0.092 | 0.010 | 0.004 | 0.237 | 0.287 | 0.001 | 0.026 | |
14.3 | 0.001 | ||||||||||
15 | 0.002 | 0.161 | 0.040 | 0.288 | 0.003 | 0.118 | 0.249 | 0.030 | 0.001 | ||
15.3 | 0.045 | ||||||||||
16 | 0.147 | 0.008 | 0.043 | 0.275 | 0.032 | 0.164 | 0.023 | ||||
16.3 | 0.036 | ||||||||||
17 | 0.038 | 0.195 | 0.192 | 0.003 | 0.041 | 0.095 | |||||
17.3 | 0.108 | 0.008 | |||||||||
18 | 0.004 | 0.093 | 0.138 | 0.001 | 0.006 | 0.203 | |||||
18.3 | 0.039 | 0.022 | |||||||||
19 | 0.102 | 0.010 | 0.131 | ||||||||
19.3 | 0.010 | 0.011 | |||||||||
20 | 0.159 | 0.001 | 0.125 | ||||||||
20.2 | 0.001 | ||||||||||
20.3 | 0.002 | 0.001 | |||||||||
21 | 0.030 | 0.106 | |||||||||
22 | 0.024 | 0.116 | |||||||||
23 | 0.135 | 0.082 | |||||||||
24 | 0.105 | 0.030 | |||||||||
25 | 0.099 | 0.014 | |||||||||
26 | 0.012 | 0.004 | |||||||||
27 | 0.002 | ||||||||||
28 | 0.001 | ||||||||||
HO | 0.719 | 0.886 | 0.756 | 0.869 | 0.795 | 0.710 | 0.798 | 0.819 | 0.778 | 0.880 | 0.777 |
HE | 0.724 | 0.891 | 0.765 | 0.875 | 0.776 | 0.719 | 0.808 | 0.808 | 0.774 | 0.883 | 0.789 |
P | 0.094 | 0.684 | 0.658 | 0.788 | 0.157 | 0.610 | 0.382 | 0.788 | 0.629 | 0.470 | 0.417 |
PM | 0.128 | 0.022 | 0.090 | 0.029 | 0.088 | 0.125 | 0.064 | 0.065 | 0.088 | 0.026 | 0.075 |
PD | 0.872 | 0.978 | 0.910 | 0.971 | 0.912 | 0.875 | 0.936 | 0.935 | 0.912 | 0.974 | 0.925 |
PIC | 0.673 | 0.881 | 0.730 | 0.862 | 0.741 | 0.671 | 0.780 | 0.784 | 0.738 | 0.871 | 0.759 |
PE | 0.458 | 0.766 | 0.521 | 0.732 | 0.590 | 0.444 | 0.596 | 0.635 | 0.559 | 0.756 | 0.557 |
TPI | 1.778 | 4.377 | 2.053 | 3.803 | 2.442 | 1.725 | 2.481 | 2.762 | 2.252 | 4.180 | 2.242 |
Allele | D16S539 | D18S51 | D19S433 | D21S11 | D22S1045 | FGA | SE33 | TH01 | TPOX | vWA |
---|---|---|---|---|---|---|---|---|---|---|
6 | 0.263 | |||||||||
7 | 0.138 | 0.001 | ||||||||
8 | 0.019 | 0.125 | 0.526 | |||||||
8.3 | 0.001 | |||||||||
9 | 0.115 | 0.001 | 0.199 | 0.103 | ||||||
9.3 | 0.265 | |||||||||
10 | 0.074 | 0.006 | 0.001 | 0.001 | 0.009 | 0.063 | ||||
11 | 0.307 | 0.016 | 0.002 | 0.134 | 0.001 | 0.001 | 0.281 | |||
12 | 0.292 | 0.110 | 0.095 | 0.013 | 0.006 | 0.026 | ||||
12.2 | 0.005 | |||||||||
13 | 0.165 | 0.138 | 0.246 | 0.003 | 0.005 | 0.001 | ||||
13.2 | 0.013 | 0.005 | ||||||||
14 | 0.026 | 0.175 | 0.324 | 0.063 | 0.025 | 0.124 | ||||
14.2 | 0.033 | 0.002 | ||||||||
15 | 0.002 | 0.149 | 0.152 | 0.381 | 0.030 | 0.100 | ||||
15.2 | 0.001 | 0.041 | ||||||||
15.3 | 0.001 | |||||||||
16 | 0.152 | 0.047 | 0.311 | 0.001 | 0.040 | 0.193 | ||||
16.2 | 0.032 | 0.001 | ||||||||
16.3 | 0.001 | |||||||||
17 | 0.120 | 0.002 | 0.079 | 0.001 | 0.077 | 0.296 | ||||
17.1 | 0.001 | |||||||||
17.2 | 0.006 | |||||||||
17.3 | 0.001 | |||||||||
18 | 0.054 | 0.008 | 0.017 | 0.083 | 0.193 | |||||
18.2 | 0.001 | |||||||||
18.3 | 0.001 | |||||||||
19 | 0.039 | 0.006 | 0.079 | 0.095 | 0.077 | |||||
19.2 | 0.001 | 0.002 | ||||||||
19.3 | 0.001 | |||||||||
20 | 0.022 | 0.118 | 0.072 | 0.016 | ||||||
20.2 | 0.001 | 0.006 | ||||||||
21 | 0.010 | 0.171 | 0.028 | |||||||
21.2 | 0.003 | 0.009 | ||||||||
22 | 0.005 | 0.171 | 0.007 | |||||||
22.2 | 0.015 | 0.031 | ||||||||
23 | 0.002 | 0.150 | 0.002 | |||||||
23.2 | 0.009 | 0.034 | ||||||||
24 | 0.001 | 0.148 | 0.001 | |||||||
24.2 | 0.002 | 0.030 | ||||||||
25 | 0.001 | 0.080 | ||||||||
25.2 | 0.002 | 0.030 | ||||||||
26 | 0.003 | 0.025 | ||||||||
26.2 | 0.037 | |||||||||
27 | 0.018 | 0.006 | 0.001 | |||||||
27.2 | 0.055 | |||||||||
28 | 0.158 | 0.001 | ||||||||
28.1 | 0.001 | |||||||||
28.2 | 0.096 | |||||||||
28.3 | 0.001 | |||||||||
29 | 0.237 | 0.007 | ||||||||
29.2 | 0.004 | 0.070 | ||||||||
30 | 0.185 | 0.001 | ||||||||
30.2 | 0.048 | 0.036 | ||||||||
31 | 0.060 | |||||||||
31.2 | 0.113 | 0.032 | ||||||||
32 | 0.008 | 0.001 | ||||||||
32.2 | 0.122 | 0.023 | ||||||||
33 | 0.002 | |||||||||
33.1 | 0.001 | |||||||||
33.2 | 0.039 | 0.008 | ||||||||
34 | 0.003 | |||||||||
34.2 | 0.004 | 0.002 | ||||||||
35 | 0.002 | |||||||||
35.2 | 0.001 | |||||||||
36 | 0.001 | |||||||||
38 | 0.001 | |||||||||
HO | 0.754 | 0.864 | 0.792 | 0.832 | 0.722 | 0.893 | 0.947 | 0.776 | 0.643 | 0.801 |
HE | 0.773 | 0.873 | 0.796 | 0.850 | 0.729 | 0.869 | 0.943 | 0.786 | 0.629 | 0.806 |
P | 0.453 | 0.226 | 0.654 | 0.125 | 0.766 | 0.987 | 0.002 | 0.981 | 0.483 | 0.121 |
PM | 0.085 | 0.030 | 0.070 | 0.041 | 0.116 | 0.032 | 0.007 | 0.079 | 0.190 | 0.064 |
PD | 0.915 | 0.970 | 0.930 | 0.959 | 0.884 | 0.968 | 0.993 | 0.921 | 0.810 | 0.936 |
PIC | 0.739 | 0.860 | 0.770 | 0.832 | 0.687 | 0.855 | 0.941 | 0.752 | 0.574 | 0.780 |
PE | 0.517 | 0.723 | 0.584 | 0.660 | 0.463 | 0.782 | 0.892 | 0.555 | 0.346 | 0.600 |
TPI | 2.035 | 3.683 | 2.404 | 2.974 | 1.798 | 4.687 | 9.469 | 2.231 | 1.402 | 2.508 |
Locus | Number of Alleles |
---|---|
CSF1PO | 8 |
D1S1656 | 17 |
D2S441 | 11 |
D2S1338 | 13 |
D3S1358 | 10 |
D5S818 | 9 |
D7S820 | 8 |
D8S1179 | 11 |
D10S1248 | 9 |
D12S391 | 17 |
D13S317 | 9 |
D16S539 | 8 |
D18S51 | 17 |
D19S433 | 15 |
D21S11 | 17 |
D22S1045 | 10 |
FGA | 20 |
SE33 | 49 |
TH01 | 8 |
TPOX | 6 |
vWA | 8 |
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Popoiu, G.; Stanciu, F.; Cuțăr, V.; Vladu, S.; Podgoreanu, P.; Nicola, V.; Stoian, I.M.; Procopciuc, A.; Hațegan, B.; Negoiță, B.; et al. Population Genetics Data of 21 Autosomal STR Loci in the Romanian Population. Data 2025, 10, 80. https://doi.org/10.3390/data10060080
Popoiu G, Stanciu F, Cuțăr V, Vladu S, Podgoreanu P, Nicola V, Stoian IM, Procopciuc A, Hațegan B, Negoiță B, et al. Population Genetics Data of 21 Autosomal STR Loci in the Romanian Population. Data. 2025; 10(6):80. https://doi.org/10.3390/data10060080
Chicago/Turabian StylePopoiu, George, Florin Stanciu, Veronica Cuțăr, Simona Vladu, Paulina Podgoreanu, Violeta Nicola, Ionel Marius Stoian, Anastasia Procopciuc, Bogdan Hațegan, Bogdan Negoiță, and et al. 2025. "Population Genetics Data of 21 Autosomal STR Loci in the Romanian Population" Data 10, no. 6: 80. https://doi.org/10.3390/data10060080
APA StylePopoiu, G., Stanciu, F., Cuțăr, V., Vladu, S., Podgoreanu, P., Nicola, V., Stoian, I. M., Procopciuc, A., Hațegan, B., Negoiță, B., Păunache, A. M., Cotolea, A., Rădulescu, A., Hubca, A. C., & Georgescu, S. E. (2025). Population Genetics Data of 21 Autosomal STR Loci in the Romanian Population. Data, 10(6), 80. https://doi.org/10.3390/data10060080