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Open AccessCommunication
Beverages 2015, 1(2), 45-54;

Determination of Osmolality in Beer to Validate Claims of Isotonicity

Erasmus Programme, Instituto de Educación Secundaria Andres de Vandelvira, Avenida Cronista Mateo y Sotos 3, 02006 Albacete, Spain
Chemisches und Veterinäruntersuchungsamt (CVUA) Karlsruhe, Weissenburger Strasse 3, 76187 Karlsruhe, Germany
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Edgar Chambers
Received: 13 March 2015 / Revised: 10 April 2015 / Accepted: 13 April 2015 / Published: 16 April 2015
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Alcohol-free beer is increasingly marketed with the claim “isotonic”. According to the European Food Safety Authority (EFSA), isotonic beverages should have an osmolality in a range of 270–330 mOsmol/kg. A method to determine osmolality in beer using an automatic cryoscope was applied and validated in this study. Isotonic and hypotonic beers can be measured directly, while hypertonic beers have to be diluted into the linear range of the instrument. As proven in several different beer matrices, the assay was linear with an average correlation coefficient of 0.998. The limits of detection and quantitation were 2 and 10 mOsmol/kg, so that the sensitivity of the method was judged sufficient to control the isotonic range. The measurement uncertainty expressed as coefficient of variation was less than 1% interday. The applicability of the method was proven by measurement of 86 beer samples. Our study has shown that the cryoscopic method is fit for the purpose to validate claims of isotonicity in food control. View Full-Text
Keywords: beer; nutritional claims; health claims; isotonic; osmolality; cryoscopy beer; nutritional claims; health claims; isotonic; osmolality; cryoscopy

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Tarancon, J.L.L.; Lachenmeier, D.W. Determination of Osmolality in Beer to Validate Claims of Isotonicity. Beverages 2015, 1, 45-54.

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