Mild Positive Pressure Improves the Efficacy of Benzalkonium Chloride against Staphylococcus aureus Biofilm
Abstract
:1. Introduction
2. Materials and Methods
2.1. Staphylococcus aureus Biofilm Production
2.2. Strategy for Proof of Concept
Preliminary Titration of Disinfectants
2.3. Combined Positive Pressure and Disinfectant Efficacy Testing
- (1)
- Diluted test disinfectant only without PP (1 atm).
- (2)
- PP only, at 3, 5, 7, and 10 atm in a positive pressure chamber designed and custom made by Dr. David Inglis (Figure 1).
- (3)
- Combined disinfectant and PP at 3, 5, 7, and 10 atm.
2.4. Confocal Laser Scanning Microscopy
2.5. Scanning Electron Microscopy
2.6. Statistical Analysis
3. Results
3.1. Effect of Positive Pressure on Benzalkonium Chloride Treatment against Staphylococcus aureus Biofilm
3.2. Confocal Laser Scanning Microscopy and Scanning Electron Microscopy
4. Discussion
4.1. Key Findings of the Study
4.2. How Does the Positive Pressure Improve the Disinfectant Killing of Biofilm Cells?
4.3. Practical Implications and Future Directions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tahir, S.; Emanuel, S.; Inglis, D.W.; Vickery, K.; Deva, A.K.; Hu, H. Mild Positive Pressure Improves the Efficacy of Benzalkonium Chloride against Staphylococcus aureus Biofilm. Bioengineering 2022, 9, 461. https://doi.org/10.3390/bioengineering9090461
Tahir S, Emanuel S, Inglis DW, Vickery K, Deva AK, Hu H. Mild Positive Pressure Improves the Efficacy of Benzalkonium Chloride against Staphylococcus aureus Biofilm. Bioengineering. 2022; 9(9):461. https://doi.org/10.3390/bioengineering9090461
Chicago/Turabian StyleTahir, Shamaila, Sarah Emanuel, David W. Inglis, Karen Vickery, Anand K. Deva, and Honghua Hu. 2022. "Mild Positive Pressure Improves the Efficacy of Benzalkonium Chloride against Staphylococcus aureus Biofilm" Bioengineering 9, no. 9: 461. https://doi.org/10.3390/bioengineering9090461