Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation and Characterization of PIC
2.2. Cell Culture
2.3. Encapsulation and 3D Culture
2.4. Bright Field Imaging and Cell Morphology Analysis
2.5. Immunofluorescence (IF) Staining and Confocal Microscope Imaging
2.6. Detection of Apoptosis (TUNEL Assay)
2.7. Statistical Analysis
3. Results
3.1. Structure and Mechanical Properties of PIC Hydrogels
3.2. Growth and Characterization of Schwann Cell in PIC Hydrogels
3.3. Growth and Characterization of Hepatocytes in PIC Hydrogels
3.4. Growth and Characterization of ECs in PIC Hydrogel
3.5. Co-Culture of ECs and Hepatocytes in PIC-RGD Enables Stable Cell Properties and EC Network Formation
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hydrogel | Polymer | [Ni2+]:[M] Ratio a | Mvb (kg mol–1) | LCc (nm) | c (mg mL–1) | RGD content (μM) | G’ at 37 °C (Pa) |
---|---|---|---|---|---|---|---|
PIC | Azide-appended PIC polymer | 1:1000 | 363 | 144 | 1 | 0 | 30 |
PIC-RGD | GRGDS-functionalized PIC polymer | 1:1000 | 363 | 144 | 1 | 28.5 | 37 |
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Ma, C.; Liu, K.; Li, Q.; Xiong, Y.; Xu, C.; Zhang, W.; Ruan, C.; Li, X.; Lei, X. Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs. Bioengineering 2022, 9, 453. https://doi.org/10.3390/bioengineering9090453
Ma C, Liu K, Li Q, Xiong Y, Xu C, Zhang W, Ruan C, Li X, Lei X. Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs. Bioengineering. 2022; 9(9):453. https://doi.org/10.3390/bioengineering9090453
Chicago/Turabian StyleMa, Chiyuan, Kaizheng Liu, Qin Li, Yue Xiong, Cuixiang Xu, Wenya Zhang, Changshun Ruan, Xin Li, and Xiaohua Lei. 2022. "Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs" Bioengineering 9, no. 9: 453. https://doi.org/10.3390/bioengineering9090453