Engineering and Monitoring 3D Cell Constructs with Time-Evolving Viscoelasticity for the Study of Liver Fibrosis In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Re-Engineering the MCTR Bioreactor
2.2. Gel Fabrication and Cell Encapsulation
2.3. Gel Water Content and Degradation
2.4. Mechanical Testing in the Low-Force MCTR Bioreactor
2.5. Cell Viability and Staining
2.6. Statistical Analysis
3. Results
4. Discussions
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Low-Force Version | Commercial Version | |
---|---|---|
Pressure regulator | Range: 0–7 kPa Resolution: 0.35 mPa | Range: 5–500 kPa Resolution: 0.1 kPa |
Membrane Hardness | 10A | 60A |
Magnet dimensions (diameter × height) | 2.5 × 1 mm | 10 × 2.5 mm |
Shuttle material | PLA | Stainless steel |
Low-Force Version | Commercial Version | |
---|---|---|
Force range | 0.02–1.4 N | 2–100 N |
Force resolution | 0.7 mN | 0.02 N |
Shuttle weight | 15 mN | 133 mN |
Viscoelastic testing | yes | yes [46] |
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Cacopardo, L.; Ahluwalia, A. Engineering and Monitoring 3D Cell Constructs with Time-Evolving Viscoelasticity for the Study of Liver Fibrosis In Vitro. Bioengineering 2021, 8, 106. https://doi.org/10.3390/bioengineering8080106
Cacopardo L, Ahluwalia A. Engineering and Monitoring 3D Cell Constructs with Time-Evolving Viscoelasticity for the Study of Liver Fibrosis In Vitro. Bioengineering. 2021; 8(8):106. https://doi.org/10.3390/bioengineering8080106
Chicago/Turabian StyleCacopardo, Ludovica, and Arti Ahluwalia. 2021. "Engineering and Monitoring 3D Cell Constructs with Time-Evolving Viscoelasticity for the Study of Liver Fibrosis In Vitro" Bioengineering 8, no. 8: 106. https://doi.org/10.3390/bioengineering8080106